The Measure of Erythropoiesis in Anemias I. The Mixing Time and the Immediate Post-Transfusion Disappearance of T-1824 Dye and of Cr51-Tagged Erythrocytes in Relation to Blood Volume Determination

Strumia, Max M.; Colwell, Louise S.; Dugan, Ann

doi:10.1182/blood.v13.2.128.128

Cited by 38 publications

(6 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…measured PV 10-15 min after injection of the indicator material without extrapolating to zero time. This would lead to higher measured values in these publications by about 3% (Strumia et al, 1958). It has now been recommended (ICSH, 1980b) that PV should be calculated by extrapolating measured albumin label values to zero time.…”

Section: Discussionmentioning

confidence: 98%

“…A reduction of 0.006 (mean of 10 observations) was made to the PCV where the buffy coat had been included in the original PCV measurement. Where the Evan's Blue measurements had been made on samples taken at 10-15 or 15-30min without extrapolation to zero time, to calculate the corrected PV a reduction of 3% and 6% respectively was made (Strumia et al 1958).…”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Interpretation of measured red cell mass and plasma volume in adults: Expert Panel on Radionuclides of the International Council for Standardization in Haematology

et al. 1995

View full text Add to dashboard Cite

Summary. Four European centres provided height and weight data on 202 males and 204 females undergoing red cell mass (RCM) and plasma volume (PV) measurements. For these populations, the RCM and PV predictions by the various published methods were compared. It was shown clearly that predictions based solely on body weight were inappropriate, particularly because approximately half of the male and female populations could be regarded as overweight or obese. Although there was reasonable agreement in the prediction values given by the formulae based on both height and weight, it was not possible to establish which formulae could be recommended. For that reason. the published literature containing normal RCM and PV measurements were re-examined. RCM data for 283 males and 171 females and PV data for 100 males and 67 females were included. Measurements were standardized for variables such as trapped plasma in the PCV, exclusion of buffy coat in the PCV and calculation of PV at zero time. As a result of this analysis, prediction formulae based on surface area for RCM and PV with 98/99% reference ranges have been established. Keywords: red cell mass, plasma volume, normal values, interpretation.Recommended methods for the measurement of red cell mass (RCM) and plasma volume (PV) have been drawn up by the Radionuclide Panel of the International Committee for Standardization in Haematology (1 980b). These methods are now widely followed and provide within-method accuracy in the order of 2-3%.The remaining problem with RCM and PV measurements is the interpretation of the results. Traditionally, results and normal values have been expressed in terms of ml per kg total body weight. Indeed, the widely quoted Polycythaemia

show abstract

Section: Discussionmentioning

confidence: 98%

Section: Methodsmentioning

confidence: 99%

Interpretation of measured red cell mass and plasma volume in adults: Expert Panel on Radionuclides of the International Council for Standardization in Haematology

et al. 1995

View full text Add to dashboard Cite

show abstract

“…Patients were transfused when a significant red cell mass deficit existed (at least 2 units of preserved red cells and as many as 6 units had to be transfused). The red cell mass deficit was estimated as the difference between the theoretical red cell mass and the actual slCr mass (measured as described below), For males the theoretical red cell volume was derived from the body surface area (ma) multiplied by 1,091 ml, and for females from the body surface area (ma) multiplied by 806 ml [20]. The body surface area was estimated from the weight and height of the patient at the time of transfusion [6].…”

Section: Recipientsmentioning

confidence: 99%

Lifespan of Preserved Red Cells¹

Szymanski

Valeri

1971

Vox Sanguinis

View full text Add to dashboard Cite

Forty-four long-term red cell survival studies were performed in 39 patients, the majority of whom had either traumatic injuries or carcinoma. With the aid of a computer, the lifespan and the rate of random destruction of preserved red cells were estimated in each recipient.The 24-hour survival was a satisfactory measurement of the percent of viable red cells in the transfusion. The mean red cell lifespan (97 days) was not affected by either the method of preservation (liquid or freeze preservation) or the length of storage at +4 or -80°C. Accelerated linear removal of red cells was seen in severely injured patients; improved red cell survival was associated with improvement in the recipient's health. The correlation between the lifespan of transfused red cells and the recipient's general health suggests that the decreased long-term survival noted in these recipients was produced by some extracorpuscular 'toxic' factor.

show abstract

“…informative than that derived at 6 min [30,33] a time period sufficient to allow for full circulatory distribution [41].…”

Section: Survival Of Compatible Kodecytes In Micementioning

confidence: 92%

Modeling transfusion reactions with kodecytes and enabling ABO‐incompatible transfusion with function‐spacer‐lipid constructs

Henry

Barr

Oliver

2012

ISBT Science Series

View full text Add to dashboard Cite

Background KODE technology uses Function‐Spacer‐Lipid (FSL) constructs for artificial attachment of blood group antigens onto live cells (kodecytes) and visualisation, thus making it potentially suitable to model both transfusion reactions and determine in vivo cell survival. Additionally, as some FSL constructs are glycolipid‐like, the possibility exists that they may also neutralise circulating antibodies, in the same way Lewis glycolipids were historically used to allow Lewis incompatible transfusions. Aims To determine if kodecytes can be used to both mimic and monitor transfusion reactions in an animal model, and also to determine if FSL construct infusions can neutralise circulating antibodies and allow for an incompatible transfusion. Methods Naïve, anti‐A positive (immunised), and anti‐A positive/FSL‐A neutralised mice were given transfusions of murine kodecytes created with FSL‐biotin and FSL‐A. Levels of surviving kodecytes were determined at multiple time‐points via their biotin label. Analysis for anti‐A was performed with inkjet printed FSL‐A constructs in a micro immunoassay. Results Normal kodecyte survival was observed in control animals. Mice with anti‐A predominantly cleared their incompatible A‐kodecyte transfusions within 6 min. Mice with anti‐A that had been neutralised with an FSL‐A infusion had normal survival of their incompatible A‐kodecyte transfusions. Re‐challenge transfusions of A‐kodecytes into mice previously given FSL‐A (once the FSL‐A had cleared from their circulation) gave two different results. One group cleared the incompatible A‐kodecytes within minutes while the other had normal survival, suggesting potential induction of tolerance. Conclusions Kodecytes can be used to model blood group A incompatible transfusions in animals. FSL‐A can neutralise anti‐A and allow for incompatible A‐kodecyte transfusion. The potential exists to extend these research observations into clinical use to allow for transfusion and transplantation across the ABO barrier, or at least mitigate the consequences of accidental ABO incompatibilities.

show abstract

The Measure of Erythropoiesis in Anemias I. The Mixing Time and the Immediate Post-Transfusion Disappearance of T-1824 Dye and of Cr51-Tagged Erythrocytes in Relation to Blood Volume Determination

Cited by 38 publications

References 20 publications

Interpretation of measured red cell mass and plasma volume in adults: Expert Panel on Radionuclides of the International Council for Standardization in Haematology

Interpretation of measured red cell mass and plasma volume in adults: Expert Panel on Radionuclides of the International Council for Standardization in Haematology

Lifespan of Preserved Red Cells¹

Modeling transfusion reactions with kodecytes and enabling ABO‐incompatible transfusion with function‐spacer‐lipid constructs

Contact Info

Product

Resources

About

The Measure of Erythropoiesis in Anemias I. The Mixing Time and the Immediate Post-Transfusion Disappearance of T-1824 Dye and of Cr51-Tagged Erythrocytes in Relation to Blood Volume Determination

Cited by 38 publications

References 20 publications

Interpretation of measured red cell mass and plasma volume in adults: Expert Panel on Radionuclides of the International Council for Standardization in Haematology

Interpretation of measured red cell mass and plasma volume in adults: Expert Panel on Radionuclides of the International Council for Standardization in Haematology

Lifespan of Preserved Red Cells1

Modeling transfusion reactions with kodecytes and enabling ABO‐incompatible transfusion with function‐spacer‐lipid constructs

Contact Info

Product

Resources

About

Lifespan of Preserved Red Cells¹