The measurement of progesterone in serum by a non-competitive idiometric assay

Barnard, Geoff; Osher, J; Lichter, S.; Gayer, Batya; Boever, Josef De; Limor, Rona; Ayalon, D.; Kohen, Förtüne

doi:10.1016/0039-128x(95)00144-f

Cited by 29 publications

(9 citation statements)

References 6 publications

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“…Therefore, for the detection of these compounds, the competitive assay format is mandatory (Haasnoot et al, 1999). Exceptions are assays based on complex anti-idiotype antibodies (Barnard et al, 1995).…”

Section: Introductionmentioning

confidence: 99%

A Direct (Non-Competitive) Immunoassay for Gentamicin Residues with an Optical Biosensor

Haasnoot¹,

Verheijen²

2001

Food and Agricultural Immunology

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Using sensor chip immobilized anti-gentamicin monoclonal antibodies in an optical biosensor (BIACORE 3000) resulted in the first single antibody based and label free noncompetitive immunoassay for the direct detection of residues of a low molecular weight compound in a food product. Calibration curves for gentamicin (Mw 466 Da) in buffer andmilk showed 50% binding at 20 and 35 ng ml -1 , respectively, which is well below the maximum residue limit of 100 ng ml -1 .

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Section: Introductionmentioning

confidence: 99%

A Direct (Non-Competitive) Immunoassay for Gentamicin Residues with an Optical Biosensor

Haasnoot¹,

Verheijen²

2001

Food and Agricultural Immunology

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“…Since the establishment of this method, Barnard's group has applied it to the detection of estradiol (Barnard & Kohen, 1990;Barnard et al, 1991;Mares et al, 1995) and progesterone (Barnard et al, 1995a) in serum and Estrone-3 Glucuronide (Barnard et al, 1995b) in urine， Kobayashi et al have applied it to the detection of UDCA 7-NAG (a bile acid metabolite) (Kobayashi et al, 2000;Kobayashi et al, 2003 a) and 11-deoxycortisol (Kobayashi et al 2003 b). More recently, Niwa et al (2009) established a noncompetitive-type ELISA for cortisol based on idiometric assay model.…”

Section: Anti-idiotype Antibody Based Noncompetitive Immunoassay (Idimentioning

confidence: 99%

Recent Progress in Noncompetitive Hapten Immunoassays: A Review

Fan¹

2012

Trends in Immunolabelled and Related Techniques

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“…The test format is called LFIA or ICG in inhibition format [69] . Several attempts are being made to reverse the response in this layout (presence of analyte yields a positive, colored response) using anti -idiotype antibodies (antibodies against antibodies) [70] , or anti -complex antibody fragments produced in an expression system [71] . A scheme of this principle and layout is presented in Figure 5.2 .…”

Section: Lateral Flow (Immuno)assaymentioning

confidence: 99%