The measurement of small magnetic signals from magnetic nanoparticles attached to the cell surface and surrounding living cells using a general-purpose SQUID magnetometer

Hashimoto, Shinji; Oda, Tatsuya; Yamada, Keiichi; Takagi, M.; Enomoto, Tsuyoshi; Ohkohchi, Nobuhiro; Takagi, Toshiyuki; Kanamori, Toshiyuki; Ikeda, Hiroshi; Yanagihara, Hideto; Kita, Eiji; Tasaki, Akira

doi:10.1088/0031-9155/54/8/021

Cited by 19 publications

(21 citation statements)

References 25 publications

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“…This is comparable to the one million cells and 1–20 μg of total nanoparticle mass typical for samples previously analyzed using much more expensive and time-consuming dosimetry based on either atomic absorption spectrometry (Settles et al, 2011) or electron paramagnetic resonance (Gamarra et al, 2010; Wilhelm et al, 2002a). Direct detection of particle magnetism with a super-conducting quantum interference device (SQUID) also requires similar cell and particulate amounts (Hashimoto et al, 2009; Levy et al, 2011). MPD, however, requires no expensive cryogens and is far simpler to operate.…”

Section: Discussionmentioning

confidence: 99%

Magnetic particle detection (MPD) for in-vitro dosimetry

Minard

Littke

Wang

et al. 2013

Biosensors and Bioelectronics

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In-vitro tests intended for evaluating the potential health effects of magnetic nanoparticles generally require an accurate measure of cell dose to promote the consistent use and interpretation of biological response. Here, a simple low-cost inductive sensor is developed for quickly determining the total mass of magnetic nanoparticles that is bound to the plasma membrane and internalized by cultured cells. Sensor operation exploits an oscillating magnetic field (f0 = 250 kHz) together with the nonlinear response of particle magnetization to generate a harmonic signal (f3 = 750 kHz) that varies linearly with particulate mass (R2 > 0.999) and is sufficiently sensitive for detecting ~100 ng of carboxyl-coated iron-oxide nanoparticles in under a second. When exploited for measuring receptor-mediated nanoparticle uptake in RAW 264.7 macrophages, results show that the achieved dosimetric performance is comparable with relatively expensive analytical techniques that are much more time-consuming and labor-intensive to perform. The described sensing is therefore potentially better suited for low-cost in-vitro assays that require fast and quantitative magnetic particle detection.

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Section: Discussionmentioning

confidence: 99%

Magnetic particle detection (MPD) for in-vitro dosimetry

Minard

Littke

Wang

et al. 2013

Biosensors and Bioelectronics

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“…In order to determine the actual heat doses administered to the in vitro tumour model we measured the amount of -Fe 2 O 3 by our original method [24]. Briefly, since the magnetic moment of -Fe 2 O 3 per unit is constant (0.49/mg emu), the amount of -Fe 2 O 3 surrounding intercellular space can be measured by detecting the magnetic moment by using a superconducting quantum interference device (SQUID) magnetometer.…”

Section: Heat Doses Administered To Heat a Cell Pellet In Vitro To Vamentioning

confidence: 99%

Minimally required heat doses for various tumour sizes in induction heating cancer therapy determined by computer simulation using experimental data

Yamada

Oda

Hashimoto

et al. 2010

International Journal of Hyperthermia

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Integrating the presently available technologies, including high-quality magnetic nanoparticles (1000 W/g(material)) and effective drug delivery systems (1-2 mg(material)/g(tumour)), treatment of a 10 mm tumour seems possible. Since treatment of smaller tumours less than 5 mm require substantial heat dose, researchers involved in IHCT should target cancer nodules of 10 mm or more, and develop a heat delivery system providing a minimum of 1.7 W/g(tumour).

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“…We previously reported on a method that can detect small magnetic moments of superparamagnetic particles attached to the cell surface and surrounding living cells by employing a superconducting quantum interference device, i.e., SQUID magnetometer [16].…”

Section: Introductionmentioning

confidence: 99%

Quantity Determination of Magnetic Particles Intravenously Administered to Mice Tissues Using Magnetization Measurements

et al. 2015

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This paper presents a method that has been developed to determine the quantity of accumulated magnetic particles in mice tissues using magnetization measurements. Dispersions of platelet Fe 3 O 4 particles with sizes of 30-50nm and a saturation magnetization of approximately 80 Am 2 /kg were intravenously administered to mice. Primary tissues were dried to measure the magnetization. The amounts of Fe 3 O 4 particles accumulated in the tissues were obtained by dividing the magnetization of tissues by the magnetization of Fe 3 O 4 particles under a magnetic field of 39.8 kA/m. A remarkable accumulation of particles was observed in the liver and spleen, being supported by the observation of tissues using Prussian blue staining. Total Fe 3 O 4 particles accumulated in primary tissues was approximately 38-40 wt% and 40-44 wt% against the particles in administered dispersions with 3.0 wt% and 0.4 wt% contents,respectively. The method developed in the study is considered to be effective for verifying magnetic hyperthermia and thermoablation therapies, in which the quantity of accumulated particles directly reflects the heating power required for those therapies.

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The measurement of small magnetic signals from magnetic nanoparticles attached to the cell surface and surrounding living cells using a general-purpose SQUID magnetometer

Cited by 19 publications

References 25 publications

Magnetic particle detection (MPD) for in-vitro dosimetry

Magnetic particle detection (MPD) for in-vitro dosimetry

Minimally required heat doses for various tumour sizes in induction heating cancer therapy determined by computer simulation using experimental data

Quantity Determination of Magnetic Particles Intravenously Administered to Mice Tissues Using Magnetization Measurements

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