The mechanism of electroacupuncture for treating spinal cord injury rats by mediating Rho/Rho-associated kinase signaling pathway

Hong, En-Si; Haihua, Yao; Min, You-jiang; Sun, Jie; Zhou, Xuan; Zeng, Xue-Bo; Wan, Yu

doi:10.1080/10790268.2019.1665612

Cited by 16 publications

(18 citation statements)

References 16 publications

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“…30 All rats were anesthetized with 3% pentobarbital sodium (1.5 mL/kg, Sigma, USA) intraperitoneally and an incision made. 31 The T 10 vertebral body of each rat was located by counting the ribs. The T 10 vertebral lamina was removed by rongeur forceps to expose the spinal cord completely.…”

Section: Modelingmentioning

confidence: 99%

“…Strike parameters consisted of a strike tip with a diameter of 3 mm, velocity of 5 m/s, retention time of 0.5 s and compression of 1.5 mm. 31 The criterion for successful model creation was one of three conditions below: rapid accumulation of edema and stagnant blood at the trauma site in the dura mater of the spinal cord, accompanied by rat tail spastic swinging, contractive vibration of the hind limb, or convulsive tics of the rat body.…”

Section: Modelingmentioning

confidence: 99%

“…The hose was fixed onto the fascia by a surgical suture, with the reveal port approximately 1 cm away from the skin; the reveal port was closed with a guide wire to prevent the overflow of cerebrospinal fluid. 31 The wound was washed with saline and penicillin, and the injured rats were raised in separate cages.…”

Section: Modelingmentioning

confidence: 99%

“…All rats were restrained with the same method and all treatments were started at 7 days following SCI. 31 In the EA treatment group, the rats were restrained on a board. Stainless steel 0.18-mm-diameter needles (Hwato Disposable Acupuncture Needle; Jiangsu Medical Supplies Factory, Jiangsu Province, China) were inserted to a depth of approximately 5 mm at the following acupoints: Yaoyangguan (GV3, posterior midline and in the depression below the spinous process of the fourth lumbar vertebra), Dazhui (GV14, posterior midline and in the depression below the spinous process of the seventh cervical vertebra) and Zusanli (ST36, bilaterally on hindlimbs below the fibular head 5 mm), Ciliao (BL32, corresponding to the second sacral posterior) on both sides.…”

Section: Grouping and Treatmentmentioning

confidence: 99%

“…After EA treatment, the rats were given normal saline by subdural injection via a microinjector from a homemade PE10 tube, each rat received 0.54 mL/kg, once per day, lasting for 14 days. 31 Rats in the Y27632 treatment group received the blocking agent Y27632 (Qilu Pharmaceutical Co., Ltd., Jinan, Shandong Province, China) by subdural injection via a microinjector from a homemade PE10 tube (18 μg Y27632 lyophilized powder was dissolved in 30 μL phosphate buffered saline solution; each rat received 0.54 mL/kg), once per day, lasting for 14 days. 31 Rats in the EA+Y treatment group received both EA and Y27632 subdural injection treatment.…”

Section: Grouping and Treatmentmentioning

confidence: 99%

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<p>Electroacupuncture Promoting Axonal Regeneration in Spinal Cord Injury Rats via Suppression of Nogo/NgR and Rho/ROCK Signaling Pathway</p>

Xiao¹,

Ding²,

Min³

et al. 2019

NDT

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To observe the changes of Nogo/NgR and Rho/ROCK signaling pathway-related gene and protein expression in rats with spinal cord injury (SCI) treated with electroacupuncture (EA) and to further investigate the possible mechanism of EA for treating SCI. Methods: Allen's method was used to create the SCI rat model. Sixty-four model rats were further subdivided into four subgroups, namely, the SCI model group (SCI), EA treatment group (EA), blocking agent Y27632 treatment group (Y27632) and EA+blocking agent Y27632 treatment group (EA+Y), according to the treatment received. The rats were subjected to EA and/or blocking agent Y27632 treatment. After 14 days, injured spinal cord tissue was extracted for analysis. The mRNA and protein expression levels were determined by real-time fluorescence quantitative PCR and Western blotting, respectively. Cell apoptosis changes in the spinal cord were evaluated by in situ hybridization. Hindlimb motor function in the rats was evaluated by Basso-Beattie-Bresnahan assessment methods. Results: Except for RhoA protein expression, compared with the SCI model group, EA, blocking agent Y27632 and EA+blocking agent Y27632 treatment groups had significantly reduced mRNA and protein expression of Nogo-A, NgR, LINGO-1, RhoA and ROCK II in spinal cord tissues, increased mRNA and protein expression of MLCP, decreased p-MYPT1 protein expression and p-MYPT1/MYPT1 ratio, and caspase3 expression, and improved lower limb movement function after treatment for 14 days (P<0.01 or <0.05). The combination of EA and the blocking agent Y27632 was superior to EA or blocking agent Y27632 treatment alone (P < 0.01 or <0.05). Conclusion: EA may have an obvious inhibitory effect on the Nogo/NgR and Rho/ROCK signaling pathway after SCI, thereby reducing the inhibition of axonal growth, which may be a key mechanism of EA treatment for SCI.

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Section: Modelingmentioning

confidence: 99%

Section: Modelingmentioning

confidence: 99%

Section: Modelingmentioning

confidence: 99%

Section: Grouping and Treatmentmentioning

confidence: 99%

Section: Grouping and Treatmentmentioning

confidence: 99%

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<p>Electroacupuncture Promoting Axonal Regeneration in Spinal Cord Injury Rats via Suppression of Nogo/NgR and Rho/ROCK Signaling Pathway</p>

Xiao¹,

Ding²,

Min³

et al. 2019

NDT

Self Cite

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Effect of Schwann cell transplantation combined with electroacupuncture on axonal regeneration and remyelination in rats with spinal cord injury

Tan

Yang

Liu

et al. 2021

The Anatomical Record

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Effect of electroacupuncture on inhibition of inflammatory response and oxidative stress through activating ApoE and Nrf2 in a mouse model of spinal cord injury

et al. 2021

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Introduction: Electroacupuncture protects neurons and myelinated axons after spinal cord injury by mitigating the inflammatory response and oxidative stress, but how it exerts these effects is unclear.Methods and results: Spinal cord injury was induced in C57BL/6 wild-type and apolipoprotein E (ApoE) knockout (ApoE -/-) mice, followed by electroacupuncture or ApoE mimetic peptide COG112 treatment. Mice with spinal cord injury suffered loss of myelinated axons and hindlimb motor function through the detections of Basso mouse scale, histology, and transmission electron microscopy; electroacupuncture partially reversed these effects in wild-type mice but not in ApoE -/mice. Combining exogenous ApoE administration with electroacupuncture significantly mitigated the effects of spinal cord injury in both mouse strains, and these effects were associated with up-regulation of anti-inflammatory cytokines and down-regulation of proinflammatory cytokines which were detected by quantitative reverse transcriptionpolymerase chain reaction. Combination treatment also reduced oxidative stress by up-regulating ApoE and Nrf2/HO-1 signaling pathway through the detections of immunofluorescence and western blot analysis. Conclusions:These results suggest that electroacupuncture protects neurons and myelinated axons following spinal cord injury through an ApoE-dependent mechanism.

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The mechanism of electroacupuncture for treating spinal cord injury rats by mediating Rho/Rho-associated kinase signaling pathway

Cited by 16 publications

References 16 publications

<p>Electroacupuncture Promoting Axonal Regeneration in Spinal Cord Injury Rats via Suppression of Nogo/NgR and Rho/ROCK Signaling Pathway</p>

<p>Electroacupuncture Promoting Axonal Regeneration in Spinal Cord Injury Rats via Suppression of Nogo/NgR and Rho/ROCK Signaling Pathway</p>

Effect of Schwann cell transplantation combined with electroacupuncture on axonal regeneration and remyelination in rats with spinal cord injury

Effect of electroacupuncture on inhibition of inflammatory response and oxidative stress through activating ApoE and Nrf2 in a mouse model of spinal cord injury

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