The mechanism of protective effect of crocin against liver mitochondrial toxicity caused by arsenic III

Abstract

In this study, we want to understand whether crocin could prevent mitochondrial damage caused by As III. For this purpose, we determined different mitochondrial toxicity endpoints caused by As III. We evaluated mitochondrial ROS formation, lipid peroxidation, mitochondrial membrane potential (MMP) collapse, mitochondrial outer membrane integrity and cytochrome c release. Our results showed that pretreatment with crocin at a concentration of 25 µg/ml significantly (p < 0.001) reduced As III-induced mitochondrial ROS formation, lipid peroxidation, MMP collapse and mitochondrial swelling. Crocin also protected the mitochondria by decreasing the mitochondrial outer membrane damage that leads to reduce the amount of cytochrome c release. These results demonstrated that crocin is a promising antidotal candidate by ameliorating As III-induced oxidative stress through mitochondrial targeting.

Crocus sativus L. Extract Containing Polyphenols Modulates Oxidative Stress and Inflammatory Response against Anti-Tuberculosis Drugs-Induced Liver Injury

“…CS contains various secondary metabolites that are responsible for different pharmacological activities. Traditionally CS has been used for various ailments like antihypertensive [10], neuroprotective [11], antitussive [12], aphrodisiac [13], antioxidant [14], antinociceptive [15], anti-inflammatory [16] activities.…”

Contrasting Role of Dose Increase in Modulating Sofosbuvir-Induced Hepatocyte Toxicity

“…Pourahmad J et, al. revealed that the hepatotoxicity caused by diclofenac is associated with oxidative stress [13]. On the other hand, liver toxicity decreases with antioxidant compounds, which is another indication of the effect of oxidative stress and free radicals on liver toxicity [14][15][16].…”

Effects of crocin in reducing DNA damage, inflammation, and oxidative stress in multiple sclerosis patients: A double‐blind, randomized, and placebo‐controlled trial

“…Interestingly, crocin is among the major active components of saffron and is a pharmacologically active and water‐soluble carotenoid isolated from saffron crocus ( Crocus sativus L .). There are plenty of studies that have exhibited that crocin possesses therapeutic and pharmaceutical properties, including high antioxidant, anti‐inflammatory, and neuroprotective activities either in vivo or in vitro . Therefore, crocin, by acting as an antioxidant or radical scavenger, could protect cells and tissues against oxidative stress damages .…”

“…There are plenty of studies that have exhibited that crocin possesses therapeutic and pharmaceutical properties, including high antioxidant, anti-inflammatory, and neuroprotective activities either in vivo or in vitro. [21][22][23][24][25] Therefore, crocin, by acting as an antioxidant or radical scavenger, could protect cells and tissues against oxidative stress damages. [1] Given the…”