The mechanism of regulation of ovarian maturation by red pigment concentrating hormone in the mud crab Scylla paramamosain

Zeng, Hui; Bao, Chenchang; Huang, Huiyang; Ye, Haihui; Li, Shaojing

doi:10.1016/j.anireprosci.2015.11.025

Cited by 39 publications

(22 citation statements)

References 51 publications

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“…A number of neuropeptides genes detected in the current study are very similar to those identified in other in silico studies that have investigated related crustacean or insect species. Confirmation of neuropeptide genes in a diverse range of species is important since several arthropod neuropeptides have an unknown function, while others have a function that has only been partially confirmed or appear to have ambiguous roles27282930. A similar recent in silico analysis approach in M. rosenbergii central nervous system yielded 21 neuropeptide transcripts (with some having multiple isoforms) that encode up to 102 mature peptides6.…”

Section: Discussionmentioning

confidence: 99%

“…In these studies, the authors implied that RPCH acts as a neurotransmitter that triggers the secretion of gonad-stimulating hormone, and can therefore induce ovarian maturation100. Recently, RPCH was reconfirmed to be involved in ovarian maturation in the mud crab S. paramamosain , possibly through a stimulatory effect on the nervous tissues30.…”

Section: Discussionmentioning

confidence: 99%

“…Still, it is important to note that in spite of the known function of some members in the CHH superfamily, their identity and functionality is vastly uncharacterised13. Lastly, there are numerous neuropeptides that have previously been demonstrated to have other functions (for example, cardio-activity, osmoregulation, pigmentation, stress response) but then later were assigned novel roles in reproduction and/or sexual development27282930. These findings highlight that while our understanding of the molecular mechanisms of neuropeptides involved in crustacean reproduction processes is quite advanced, it is yet still far from being complete.…”

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confidence: 99%

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Transcriptomic characterization and curation of candidate neuropeptides regulating reproduction in the eyestalk ganglia of the Australian crayfish, Cherax quadricarinatus

Nguyen

Cummins

Elizur

et al. 2016

Sci Rep

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The Australian redclaw crayfish (Cherax quadricarinatus) has recently received attention as an emerging candidate for sustainable aquaculture production in Australia and worldwide. More importantly, C. quadricarinatus serves as a good model organism for the commercially important group of decapod crustaceans as it is distributed worldwide, easy to maintain in the laboratory and its reproductive cycle has been well documented. In order to better understand the key reproduction and development regulating mechanisms in decapod crustaceans, the molecular toolkit available for model organisms such as C. quadricarinatus must be expanded. However, there has been no study undertaken to establish the C. quadricarinatus neuropeptidome. Here we report a comprehensive study of the neuropeptide genes expressed in the eyestalk in the Australian crayfish C. quadricarinatus. We characterised 53 putative neuropeptide-encoding transcripts based on key features of neuropeptides as characterised in other species. Of those, 14 neuropeptides implicated in reproduction regulation were chosen for assessment of their tissue distribution using RT-PCR. Further insights are discussed in relation to current knowledge of neuropeptides in other species and potential follow up studies. Overall, the resulting data lays the foundation for future gene-based neuroendocrinology studies in C. quadricarinatus.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

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Transcriptomic characterization and curation of candidate neuropeptides regulating reproduction in the eyestalk ganglia of the Australian crayfish, Cherax quadricarinatus

Nguyen

Cummins

Elizur

et al. 2016

Sci Rep

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“…RPCH transcripts were detected in ovaries and heart in addition to the neural tissue in L. vannamei ( 28 ), supportive of a role for this hormone in reproduction. In vitro co-incubation of ovary explant with nervous tissue caused significant oocyte growth when RPCH was added to the explant culture in several crustacean species ( 38 , 103 , 104 ). Fingerman ( 38 ) further confirmed that the combined effects of RPCH and thoracic ganglia on ovarian maturation was more significant compared to that with explants incubated with thoracic ganglia alone; this suggested that RPCH, as proposed for serotonin, acts as neurotransmitter stimulating the release of VSH, with calcium acting as a second messenger for RPCH.…”

Section: Red Pigment-concentrating Hormone (Rpch)mentioning

confidence: 99%

Insights on Molecular Mechanisms of Ovarian Development in Decapod Crustacea: Focus on Vitellogenesis-Stimulating Factors and Pathways

2020

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Vitellogenesis in crustaceans is an energy-consuming process. Though the underlying mechanisms of ovarian maturation in decapod Crustacea are still unclear, evidence indicates the process to be regulated by antagonistically-acting inhibitory and stimulating factors specifically originating from X-organ/sinus gland (XO/SG) complex. Among the reported neuromediators, neuropeptides belonging to the crustacean hyperglycemic hormone (CHH)-family have been studied extensively. The structure and dynamics of inhibitory action of vitellogenesis-inhibiting hormone (VIH) on vitellogenesis have been demonstrated in several species. Similarly, the stimulatory effects of other neuropeptides of the CHH-family on crustacean vitellogenesis have also been validated. Advancement in transcriptomic sequencing and comparative genome analysis has led to the discovery of a large number of neuromediators, peptides, and putative peptide receptors having pleiotropic and novel functions in decapod reproduction. Furthermore, differing research strategies have indicated that neurotransmitters and steroid hormones play an integrative role by stimulating neuropeptide secretion, thus demonstrating the complex intertwining of regulatory factors in reproduction. However, the molecular mechanisms by which the combinatorial effect of eyestalk hormones, neuromediators and other factors coordinate to regulate ovarian maturation remain elusive. These multifunctional substances are speculated to control ovarian maturation possibly via the autocrine/paracrine pathway by acting directly on the gonads or by indirectly exerting their stimulatory effects by triggering the release of a putative gonad stimulating factor from the thoracic ganglion. Acting through receptors, they possibly affect levels of cyclic nucleotides (cAMP and cGMP) and Ca 2+ in target tissues leading to the regulation of vitellogenesis. The “stimulatory paradox” effect of eyestalk ablation on ovarian maturation continues to be exploited in commercial aquaculture operations, and is outweighed by the detrimental physiological effects of this procedure. In this regard, the development of efficient alternatives to eyestalk ablation based on scientific knowledge is a necessity. In this article, we focus principally on the signaling pathways of positive neuromediators and other factors regulating crustacean reproduction, providing an overview of their proposed receptor-mediated stimulatory mechanisms, intracellular signaling, and probable interaction with other hormonal signals. Finally, we provide insight into future research directions on crustacean reproduction as well as potential applications of such research to aquaculture technology development.

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“…The members of the CHH family, including CHH, MIH, and VIH, have been shown to be involved in carbohydrate and lipid metabolism, molting, reproduction, and osmotic regulation in decapod crustaceans (Böcking, Dircksen, & Keller, ). The RPCH is not only involved in the regulation of pigmentation but also in the regulation of ovarian maturation in crustaceans (Fingerman, ), such as in mud crab (Zeng, Bao, Huang, Ye, & Li, ). Ecdysone receptor (EcR) and farnesoic acid O‐methyltransferase (FAMeT) are important genes and have a high expression in the eyestalk of mud crab (Gong et al, ; Y. Yang, Ye, Huang, Jin, & Li, ).…”

Section: Introductionmentioning

confidence: 99%

miR‐34 regulates reproduction by inhibiting the expression of MIH, CHH, EcR, and FAMeT genes in mud crab Scylla paramamosain

Zhou

Jia

Wan

et al. 2018

Molecular Reproduction Devel

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Mud crab Scylla paramamosain is a commercially important species widely cultured in China. It is well known that the eyestalk regulates reproductive activities in crustaceans. In our previous research, we found that the miR‐34 expression level in male eyestalk was significantly higher than that in females. Thus, we assumed that it may play an important role in regulating reproduction. In this study, we used bioinformatic tools to identify the target genes of miR‐34 in eyestalk. Six reproduction‐related genes with an intact 3′‐untranslated region (UTR), including molt‐inhibiting hormone (MIH), crustacean hyperglycemic hormone (CHH), vitellogenesis‐inhibiting hormone, red pigment concentrating hormone, ecdysone receptor (EcR), and farnesoic acid methyltransferase (FAMeT) were identified. When the 3′‐UTR plasmid vectors of the six genes were cotransfected with miR‐34 mimics into 293FT cells, respectively, the luciferase activities of four genes (MIH, CHH, EcR, and FAMeT) were significantly decreased compared with that in the control group; on the contrary, when the six plasmid vectors were cotransfected with the miR‐34 inhibitor respectively, the luciferase activities of four genes (MIH, CHH, EcR, and FAMeT) were significantly higher than that in the control group. When agomiR‐34 and antagomiR‐34 were injected into the eyestalk respectively in vivo, the expression levels of the MIH, CHH, EcR, and FAMeT genes were detected by a quantitative real‐time polymerase chain reaction. The results showed that agomiR‐34 suppressed the expression of the four genes, whereas antagomiR‐34 enhanced their expression. These experimental results confirmed our hypothesis that miR‐34 may indirectly regulate reproduction via binding to the 3′‐UTRs of MIH, CHH, EcR, and FAMeT genes and suppressing their expression.

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The mechanism of regulation of ovarian maturation by red pigment concentrating hormone in the mud crab Scylla paramamosain

Cited by 39 publications

References 51 publications

Transcriptomic characterization and curation of candidate neuropeptides regulating reproduction in the eyestalk ganglia of the Australian crayfish, Cherax quadricarinatus

Transcriptomic characterization and curation of candidate neuropeptides regulating reproduction in the eyestalk ganglia of the Australian crayfish, Cherax quadricarinatus

Insights on Molecular Mechanisms of Ovarian Development in Decapod Crustacea: Focus on Vitellogenesis-Stimulating Factors and Pathways

miR‐34 regulates reproduction by inhibiting the expression of MIH, CHH, EcR, and FAMeT genes in mud crab Scylla paramamosain

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