The mechanisms and diagnostic potential of lncRNAs, miRNAs, and their related signaling pathways in cervical cancer

Xu, Yan; Sun, Yu; Song, Xin; Ren, Jian

doi:10.3389/fcell.2023.1170059

Cited by 5 publications

(2 citation statements)

References 116 publications

(113 reference statements)

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“…We identified a predominantly cytoplasmic localization for the majority of differentially expressed lncRNAs. These lncRNAs can be involved in post-transcriptional regulation through their influence on the stability of mRNAs, act as translation regulators while forming mRNA-lncRNA complexes and can release miRNAs from their target genes as miRNA "sponges" (Xu Y. et al, 2023). All these processes may be impaired in cervical cancer, so it is important to further investigate the molecular mechanisms of function of lncRNAs selected in this study.…”

Section: Discussionmentioning

confidence: 99%

mRNA-lncRNA gene expression signature in HPV-associated neoplasia and cervical cancer

Kulaeva,

Muzlaeva,

Mashkina

2024

Vestn. VOGiS

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Cervical cancer is one of the most frequent cancers in women and is associated with human papillomavirus (HPV) in 70 % of cases. Cervical cancer occurs because of progression of low-differentiated cervical intraepithelial neoplasia through grade 2 and 3 lesions. Along with the protein-coding genes, long noncoding RNAs (lncRNAs) play an important role in the development of malignant cell transformation. Although human papillomavirus is widespread, there is currently no well-characterized transcriptomic signature to predict whether this tumor will develop in the presence of HPV-associated neoplastic changes in the cervical epithelium. Changes in gene activity in tumors reflect the biological diversity of cellular phenotype and physiological functions and can be an important diagnostic marker. We performed comparative transcriptome analysis using open RNA sequencing data to assess differentially expressed genes between normal tissue, neoplastic epithelium, and cervical cancer. Raw data were preprocessed using the Galaxy platform. Batch effect correction, identification of differentially expressed genes, and gene set enrichment analysis (GSEA) were performed using R programming language packages. Subcellular localization of lncRNA was analyzed using Locate-R and iLoc-LncRNA 2.0 web services. 1,572 differentially expressed genes (DEGs) were recorded in the “cancer vs. control” comparison, and 1,260 DEGs were recorded in the “cancer vs. neoplasia” comparison. Only two genes were observed to be differentially expressed in the “neoplasia vs. control” comparison. The search for common genes among the most strongly differentially expressed genes among all comparison groups resulted in the identification of an expression signature consisting of the CCL20, CDKN2A, CTCFL, piR-55219, TRH, SLC27A6 and EPHA5 genes. The transcription level of the CCL20 and CDKN2A genes becomes increased at the stage of neoplastic epithelial changes and stays so in cervical cancer. Validation on an independent microarray dataset showed that the differential expression patterns of the CDKN2A and SLC27A6 genes were conserved in the respective gene expression comparisons between groups.

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Section: Discussionmentioning

confidence: 99%

mRNA-lncRNA gene expression signature in HPV-associated neoplasia and cervical cancer

Kulaeva,

Muzlaeva,

Mashkina

2024

Vestn. VOGiS

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“…As a result, they play a crucial role in the genesis and progression of numerous malignant tumors, including ovarian cancer 1 - 4 . Thus, lncRNAs hold promising potential as therapeutic targets and molecular markers for prognostic monitoring in various malignant tumors 5 - 9 . Our preceding research illustrated that elevated expressions of long non-coding RNA LINC01021 (officially known as p53 upregulated regulator of p53 level, abbreviated as PURPL) were present in epithelial ovarian cancer tissues.…”

Section: Introductionmentioning

confidence: 99%

Abnormal expressions of PURPL, miR-363-3p and ADAM10 predicted poor prognosis for patients with ovarian serous cystadenocarcinoma

Zhang,

Guo,

Zhao

et al. 2023

J. Cancer

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Objective: This study aimed to elucidate the prognostic implications of deviant expressions of long non-coding RNA (lncRNA) p53 upregulated regulator of p53 levels (PURPL), microRNA-363-3p (miR-363-3p), and ADAM metallopeptidase domain 10 (ADAM10) in patients diagnosed with ovarian serous cystadenocarcinoma (OSC). Methods: To predict and refine the targeted miRNAs and downstream target genes for PURPL, we utilized open medical databases. Through the employment of real-time RT-PCR, we conducted tissue analysis to discern the expressions of PURPL, miR-363-3p, and ADAM10 in both OSC and control tissues. The pathological correlations in the clinic and the prognostic implications of deviant expressions of PURPL, miR-363-3p, and ADAM10 in OSC patients were analyzed independently. Results: Database inquiries revealed that PURPL might target miR-363-3p, and in turn, miR-363-3p could target ADAM10. Differential expression of PURPL, miR-363-3p, and ADAM10 was observed between OSC and paired tissues. The premature version of miR-363-3p, miR-363, correlated with overall survival (OS), while ADAM10 corresponded with progression-free survival (PFS) in ovarian cancer patients. Tissue detection displayed significantly elevated expressions of PURPL and ADAM10, and conspicuously diminished expressions of miR-363-3p in OSC tissues compared to the control tissues (P<0.05). A negative correlation was observed between the expressions of PURPL and miR-363-3p, and miR-363-3p and ADAM10, while a positive correlation was found between PURPL and ADAM10 in different ovarian tissues (P<0.05). In OSC tissues, upregulation of PURPL was associated with an advanced clinical stage, TP53 mutation, and lymph node metastasis (P<0.05), downregulation of miR-363-3p was associated with a more advanced clinical stage and lymph node metastasis (P<0.05), and overexpression of ADAM10 correlated with a more advanced FIGO stage. High expressions of PURPL and ADAM10, and low expression of miR-363-3p, were linked with poor PFS and OS in OSC patients, respectively (P<0.05). In addition, OSC patients with elevated PURPL and reduced miR-363-3p, patients with elevated PURPL and ADAM10, and patients with reduced miR-363-3p and elevated ADAM10 also demonstrated worse PFS and OS, respectively (P<0.05). Conclusions: The anomalous expressions of PURPL, miR-363-3p, and ADAM10 might contribute to the pathogenesis of OSC via up-down stream regulation, and these abnormal expressions could serve as potential prognostic indicators for OSC patients.

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Pivotal Role of miRNA–lncRNA Interactions in Human Diseases

Pooresmaeil,

Azadi,

Hasannejad-Asl

et al. 2024

Mol Biotechnol

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The mechanisms and diagnostic potential of lncRNAs, miRNAs, and their related signaling pathways in cervical cancer

Cited by 5 publications

References 116 publications

mRNA-lncRNA gene expression signature in HPV-associated neoplasia and cervical cancer

mRNA-lncRNA gene expression signature in HPV-associated neoplasia and cervical cancer

Abnormal expressions of PURPL, miR-363-3p and ADAM10 predicted poor prognosis for patients with ovarian serous cystadenocarcinoma

Pivotal Role of miRNA–lncRNA Interactions in Human Diseases

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