The meningococcus and mechanisms of pathogenicity.

DeVoe, I W

doi:10.1128/mmbr.46.2.162-190.1982

Cited by 115 publications

(78 citation statements)

References 153 publications

(248 reference statements)

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“…The effeel of capsules on MBP binding is further illustrated by \he difference of MBP binding capacity between serogroup A and the other meningoeoccal serogroups. The capsule of serogroup A contains repeating units of partially o-acetylated (1 6)linked 2-acetamido-2-deoxy-D-mannopyranosyl phosphate groups which is not present in other meningoeoccal serogroups [26][27][28]. This serogroup A structure potentially exposes ManNAc, and it seems likely that this is the ligand for MBP (since it is known to be a good ligand for MBP} in the capsular structure [26.27].…”

Section: Discussionmentioning

confidence: 99%

Binding of mannan-binding protein to various bacterial pathogens of meningitis

Emmerik

Kuijper

Fijen

et al. 1994

Clinical and Experimental Immunology

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SUMMARYMannan-binding protein (MBP), a calcium-dependent plasma lectin, may play a role in the innate defence against microorganisms. After binding lo carbohydrate structures at the bacterial surface, MBP activates the classical pathway of the complement system. To investigate the binding capacity of MBP to various bacteria associated with meningitis, an assay was developed to study the binding of MBP to bacteria grown in a semisynthctic lluid culture medium. Salmonella montevideo (containing a mannose-rich lipopolysaceharide (EPS)), used as a positive control strain, showed binding of radioiabclled MBP at a level of 80% compared with binding of MBP to zymosan. Binding of labelled MBP to Salm. monievideo was time-dependent, temperaturedependent and saturablc. The binding, was inhibited by unlabelled MBP., by mannose and by Nacetyl-ij-glucosamine. Among bacterial pathogens often found to cause meningitis, a wide range of MBP binding capacities could be determined. The encapsulated Neisseriu meningitidis (representatives from 11 serogroups other than group A were included: n ~ 22), N. mucosa {n = \), iUicmophilus mfiuenzae type b \n = 10) and Streptococcus agalactiae [n = 5) had a low MBP binding capacity of21-7% (95% confidence interval (Cl)3'3 4i)\%). Escheriehia coliK\ (« = 11).Strep, suis {n = 5), Strep, pneumoniae (n = 10) and N. meningitidis scrogroup A (n = 2) showed intermediate MBP binding capacity of 58-4% (95% Cl 40-0-76 8%). A third group consisting of non-encapsulated Listeria monocytogenes (rt = 11), non-encapsulated H. injluenzae (n = 2), nonencapsulated N. meningitidis (n = 2), N. cinera {n = \) and N. .subflava {n = \) strains had a high MBP binding capacity of 875% (95% C! 62'5 112 5%). The majority of encapsulated pathogens causing bacterial meningitis seem to have a rather low MBP binding capacity.

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Section: Discussionmentioning

confidence: 99%

Binding of mannan-binding protein to various bacterial pathogens of meningitis

Emmerik

Kuijper

Fijen

et al. 1994

Clinical and Experimental Immunology

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“…The mechanisms which enable these pathogens to disseminate within susceptible individuals are not fully understood. From the sequence of events noted in patients with meningococcal disease, it appears that the bacterium enters the systemic circulation from the oropharynx and disseminates by the haematogenous route to other sites throughout the body (DeVoe, 1982).…”

Section: Introductionmentioning

confidence: 99%

“…Analysis of autopsy material from human disease has shown extensive damage to the endotheiiai lining of the blood vessels (Hill and Kinney, 1947). One bacterial component responsible for toxicity to the endotheiiai cells may be the lipopolysaccharide {DeVoe, 1982). However, to date, the role of the other outer membrane components in the interactions of this pathogen with host endotheiiai cells and their contribution to pathogenesis have not been investigated.…”

Section: Introductionmentioning

confidence: 99%

The role of pili in the interactions of pathogenic Neisseria with cultured human endothelial cells

Virji

Käyhty

Ferguson

et al. 1991

Molecular Microbiology

211

196

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The influence of the two surface structures of Neisseria meningitidis, capsule and pili, in bacterial interactions with human endothelial cells was investigated. Increased association correlated with the presence of pili on bacteria while capsule type had no apparent effect. Strains expressing both Class I and Class II pili associated with endothelial cells in significantly larger numbers compared with the non-piliated variants of the same strains (greater than 10x). Variants of Neisseria gonorrhoeae strain P9 expressing antigenically distinct pili also associated with endothelial cells in larger numbers (greater than 30x) compared with the non-piliated variant. Electron microscopic studies confirmed these data and showed that gonococci were internalized more frequently compared with meningococci. One consequence of increased association was an increase in the cytopathic effect of bacteria on the target cells.

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“…Transformation-mediated exchange may allow pathogenic organisms to adapt to their host, but may also incidentally enhance their pathogenic potential. For example, only some capsular types have strong pathogenic potential and are preferentially associated with meningitis [29]. Up to 30% of the human population carry meningococci in their nasopharyngeal tract without being diseased.…”

Section: Resultsmentioning

confidence: 99%

Transformation-mediated exchange of virulence determinants by co-cultivation of pathogenic Neisseriae

Frosch¹

1992

FEMS Microbiology Letters

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The horizontal flow of genetic material between microbes utilizes three principal routes: conjugation, transduction and transformation. While the significance in nature of the first two pathways is generally accepted, the in vivo role of transformation remains uncertain, despite the early observations by Griffith in 1928 on the transformation of streptococci from an avirulent to a virulent state [1]. Recently, circumstantial evidence was collected suggesting a role for transformation-mediated horizontal exchange in the modulation of virulence determinants of pathogenic Neisseriae and the variation of surface structures. In order to further assess the significance of transformation-mediated exchange we performed simple co-cultivation experiments of different Neisseria strains. We observed an efficient intra- and interspecies transfer of essential virulence determinants; the process was sensitive to the presence of DNaseI in the culture and was blocked in transformation-deficient recipients.

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The meningococcus and mechanisms of pathogenicity.

Cited by 115 publications

References 153 publications

Binding of mannan-binding protein to various bacterial pathogens of meningitis

Binding of mannan-binding protein to various bacterial pathogens of meningitis

The role of pili in the interactions of pathogenic Neisseria with cultured human endothelial cells

Transformation-mediated exchange of virulence determinants by co-cultivation of pathogenic Neisseriae

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