The MERS-CoV N Protein Regulates Host Cytokinesis and Protein Translation via Interaction With EF1A

Zhu, Lin; Gao, Ting; Fu, Yangbo; Han, Xiujing; Yue, Junjie; Liu, Yaoning; Liu, Hainan; Dong, Qincai; Yang, Wei; Hu, Yong; Jin, Yu; Li, Ping; Li, Xuan; Cao, Cheng

doi:10.3389/fmicb.2021.551602

Cited by 4 publications

(3 citation statements)

References 40 publications

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“…4f, g ). Several studies have suggested that the NP of HCoVs may trigger cellular apoptosis, 32 – 34 which is in line with our results. Additionally, we further demonstrated that Seq-1022 could effectively hinder apoptosis induced by NP in 16HBE cells by interfering with its functionality (Supplementary Fig.…”

Section: Resultssupporting

confidence: 93%

Aptamers targeting SARS-CoV-2 nucleocapsid protein exhibit potential anti pan-coronavirus activity

Yang,

Li,

et al. 2024

Sig Transduct Target Ther

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Emerging and recurrent infectious diseases caused by human coronaviruses (HCoVs) continue to pose a significant threat to global public health security. In light of this ongoing threat, the development of a broad-spectrum drug to combat HCoVs is an urgently priority. Herein, we report a series of anti-pan-coronavirus ssDNA aptamers screened using Systematic Evolution of Ligands by Exponential Enrichment (SELEX). These aptamers have nanomolar affinity with the nucleocapsid protein (NP) of Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and also show excellent binding efficiency to the N proteins of both SARS, MERS, HCoV-OC43 and -NL63 with affinity KD values of 1.31 to 135.36 nM. Such aptamer-based therapeutics exhibited potent antiviral activity against both the authentic SARS-CoV-2 prototype strain and the Omicron variant (BA.5) with EC50 values at 2.00 nM and 41.08 nM, respectively. The protein docking analysis also evidenced that these aptamers exhibit strong affinities for N proteins of pan-coronavirus and other HCoVs (−229E and -HKU1). In conclusion, we have identified six aptamers with a high pan-coronavirus antiviral activity, which could potentially serve as an effective strategy for preventing infections by unknown coronaviruses and addressing the ongoing global health threat.

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Section: Resultssupporting

confidence: 93%

Aptamers targeting SARS-CoV-2 nucleocapsid protein exhibit potential anti pan-coronavirus activity

Yang,

Li,

et al. 2024

Sig Transduct Target Ther

View full text Add to dashboard Cite

show abstract

“…MERS-CoV N protein can also act as an IFN antagonist, inhibiting type I and type III interferon induction by targeting RIG-I signalling [ 44 ]. In the host cell life cycle, MERS-CoV N protein interacts with human translation elongation factor 1A(EF1A) to inhibit cytokinesis and cell proliferation [ 45 ]. These findings imply that N protein may play a role in pathogenesis.…”

Section: Discussionmentioning

confidence: 99%

Genetic diversity and molecular epidemiology of Middle East Respiratory Syndrome Coronavirus in dromedaries in Ethiopia, 2017–2020

Zhou

Ali

Walelign³

et al. 2023

Emerging Microbes & Infections

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Middle East respiratory syndrome coronavirus (MERS-CoV) is enzootic in dromedary camels and causes zoonotic infection and disease in humans. Although over 80% of the global population of infected dromedary camels are found in Africa, zoonotic disease had only been reported in the Arabia Peninsula and travel-associated disease has been reported elsewhere. In this study, genetic diversity and molecular epidemiology of MERS-CoV in dromedary camels in Ethiopia were investigated during 2017–2020. Of 1766 nasal swab samples collected, 61 (3.5%) were detected positive for MERS-CoV RNA. Of 484 turbinate swab samples collected, 10 (2.1%) were detected positive for MERS-CoV RNA. Twenty-five whole genome sequences were obtained from these MERS-CoV positive samples. Phylogenetically, these Ethiopian camel-originated MERS-CoV belonged to clade C2, clustering with other East African camel strains. Virus sequences from camel herds clustered geographically while in an abattoir, two distinct phylogenetic clusters of MERS-CoVs were observed in two sequential sampling collections, which indicates the greater genetic diversity of MERS-CoV in abattoirs. In contrast to clade A and B viruses from the Arabian Peninsula, clade C camel-originated MERS-CoV from Ethiopia had various nucleotide insertions and deletions in non-structural gene nsp3, accessory genes ORF3 and ORF5 and structural gene N. This study demonstrates the genetic instability of MERS-CoV in dromedaries in East Africa, which indicates that the virus is still actively adapting to its camel host. The impact of the observed nucleotide insertions and deletions on virus evolution, viral fitness, and zoonotic potential deserves further study.

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“…SARS-CoV-2 is a coronavirus with a single-stranded positive-sense RNA genome that can infect a wide range of vertebrates, including wild animals, domestic animals and humans ( 1 – 3 ). Coronaviruses receive their name for the surface of each virion outer membrane is decorated with characteristic “crown-like” spikes that bind to host receptors and confer specificity and infectivity ( 4 , 5 ).…”

Section: Introductionmentioning

confidence: 99%

Translational Control of COVID-19 and Its Therapeutic Implication

et al. 2022

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The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the causative agent of COVID-19, which has broken out worldwide for more than two years. However, due to limited treatment, new cases of infection are still rising. Therefore, there is an urgent need to understand the basic molecular biology of SARS-CoV-2 to control this virus. SARS-CoV-2 replication and spread depend on the recruitment of host ribosomes to translate viral messenger RNA (mRNA). To ensure the translation of their own mRNAs, the SARS-CoV-2 has developed multiple strategies to globally inhibit the translation of host mRNAs and block the cellular innate immune response. This review provides a comprehensive picture of recent advancements in our understanding of the molecular basis and complexity of SARS-CoV-2 protein translation. Specifically, we summarize how this viral infection inhibits host mRNA translation to better utilize translation elements for translation of its own mRNA. Finally, we discuss the potential of translational components as targets for therapeutic interventions.

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The MERS-CoV N Protein Regulates Host Cytokinesis and Protein Translation via Interaction With EF1A

Cited by 4 publications

References 40 publications

Aptamers targeting SARS-CoV-2 nucleocapsid protein exhibit potential anti pan-coronavirus activity

Aptamers targeting SARS-CoV-2 nucleocapsid protein exhibit potential anti pan-coronavirus activity

Genetic diversity and molecular epidemiology of Middle East Respiratory Syndrome Coronavirus in dromedaries in Ethiopia, 2017–2020

Translational Control of COVID-19 and Its Therapeutic Implication

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