The Micro-RNA Expression Profiles of Autoimmune Arthritis Reveal Novel Biomarkers of the Disease and Therapeutic Response

Dudics, Steven; Venkatesha, Shivaprasad H.; Moudgil, Kamal D.

doi:10.3390/ijms19082293

Cited by 32 publications

(45 citation statements)

References 81 publications

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“…Most of the dysregulated miRNAs in CAP, such as miR-146a (Ichii et al 2012;Jang et al 2016;Bhardwaj 2017;Shi et al 2019), miR-96 (Dudics et al 2018), miR-423 (Wang et al 2017;Nakhjavani et al 2019) and miR-205 (Suojalehto et al 2013), their expression in various previously reported inflammatory diseases showed similar patterns to this study. It has been found that miR-146, miR-96, miR-423, and miR-205 are closely related to prostate cancer (Song et al 2018).…”

Section: Discussionsupporting

confidence: 86%

The effect of Jiedu Huoxue decoction on rat model of experimental nonbacterial prostatitis via regulation of miRNAs

Yan

Huang

et al. 2020

Pharmaceutical Biology

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Context: The underlying mechanisms of Jiedu Huoxue decoction (JDHXD) in treating chronic prostatitis have not been fully explored. Objective: This study investigates the miRNAs as potential biomarkers and the effect of JDHXD on the rat model of experimental nonbacterial prostatitis. Materials and methods: Fifty-four Sprague-Dawley male rats were randomly divided into normal control, model, JDHXD low dose (0.5 g/kg/day), medium dose (1 g/kg/day), high dose (2 g/kg/day) and western medicine (cernilton 0.094 g/kg/day) groups, and intragastrically administered once daily for 30 days. The control and model (upon successful establishment) groups received distilled water. Differential expression of miRNAs was analysed with high-throughput miRNA sequencing and validated with qRT-PCR and Northern blot. Prediction of specific target genes and functional enrichment analysis were performed with bioinformatics. Results: LD 50 test showed no sign of toxicity with maximum feasible dose 4 g/kg JDHXD. Compared with control, 495 miRNAs showed expression changes in CAP/CPPS rats, of which 211 were significantly different and 37 were prostatic-related. There were 181 differentially expressed miRNAs between the model and high dose JDHXD groups, of which 23 were identical with the control and model groups. Compared with control, miR-146a, miR-423 and miR-205 expression increased significantly in the model group, decreased dose-dependently in the JDHXD groups (p < 0.05), and vice-versa for miR-96 (p < 0.05). The effect of low dose JDHXD was comparable to cernilton (p > 0.05). Discussion and conclusions: Future studies may explore the contributions of the active components in JDHXD. The study design is generalisable. The effect can be repeatedly verified in clinical trials.

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Section: Discussionsupporting

confidence: 86%

The effect of Jiedu Huoxue decoction on rat model of experimental nonbacterial prostatitis via regulation of miRNAs

Yan

Huang

et al. 2020

Pharmaceutical Biology

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“…Earlier studies on animal models have also confirmed the role of mir-155 as one of the factors contributing to arthritis development. Results of serum testing in arthritic rats showed an increased level of miR-155; on the other hand, mice lacking miR-155 were resistant to collagen-induced arthritis [ 14 , 15 ]. In the present study, we decided to focus on microRNAs since they are responsible for regulating gene expression of transcriptional factors that impact Th17/Treg balance [ 2 , 12 ].…”

Section: Discussionmentioning

confidence: 99%

“…In our study, we have shown a correlation between miR-155 and STAT3 as well as between miR-26 and STAT3, SMAD3, and SOCS1 in RA Th17 cells. Che et al have shown that miR-155 shows the potential to be used as a biomarker [ 14 ]. For RA Treg cells, we found a correlation between miR-155 with SMAD3 and SMAD4, which again confirms the possibility to use miR-155 as a biomarker [ 16 , 17 , 18 , 19 ].…”

Section: Discussionmentioning

confidence: 99%

The Interplay between Transcriptional Factors and MicroRNAs as an Important Factor for Th17/Treg Balance in RA Patients

Kmiołek

Rzeszotarska

Wajda

et al. 2020

IJMS

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MicroRNAs regulate gene expression of transcriptional factors, which influence Th17/Treg (regulatory T cells) balance, establishing the molecular mechanism of genetic and epigenetic regulation of Treg and Th17 cells is crucial for understanding rheumatoid arthritis (RA) pathogenesis. The study goal was to understand the potential impact of the selected microRNAs expression profiles on Treg/Th17 cells frequency, RA phenotype, the expression profile of selected microRNAs, and their correlation with the expression profiles of selected transcriptional factors: SOCS1, SMAD3, SMAD4, STAT3, STAT5 in RA; we used osteoarthritis (OA) and healthy controls (HCs) as controls. The study was conducted on 14 RA and 11 OA patients, and 15 HCs. Treg/Th17 frequency was established by flow cytometry. Gene expression analysis was estimated by qPCR. We noticed correlations in RA Th17 cells between miR-26 and SMAD3, STAT3, SOCS1; and miR-155 and STAT3—and in RA Treg cells between miR-26 and SOCS1; miR-31, -155 and SMAD3; and miR-155 and SMAD4. In RA Tregs, we found a negative correlation between miR-26, -126 and STAT5a. The expression level of miR-31 in Th17 cells from RA patients with DAS28 ≤ 5.1 is higher and that for miR-24 is greater in Tregs from patients with DAS28 > 5.1. MiR-146a in Tregs is higher in rheumatoid factor (RF) positive RA patients.

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“…This initiates both a B and T cell response to CII, leading to arthritis induction [ 32 , 33 ]. Another commonly used model is the adjuvant-induced arthritis (AA) model [ 34 , 35 ]. In AA, Lewis rats are immunized with heat-killed Mycobacterium tuberculosis H 37 R a (Mtb) emulsified in mineral oil.…”

Section: Animal Models Of Ra For Studying Disease Pathogenesis Andmentioning

confidence: 99%

Natural Products for the Treatment of Autoimmune Arthritis: Their Mechanisms of Action, Targeted Delivery, and Interplay with the Host Microbiome

Dudics

Langan

Meka

et al. 2018

IJMS

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129

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Rheumatoid arthritis (RA) is a chronic, debilitating illness characterized by painful swelling of the joints, inflammation of the synovial lining of the joints, and damage to cartilage and bone. Several anti-inflammatory and disease-modifying drugs are available for RA therapy. However, the prolonged use of these drugs is associated with severe side effects. Furthermore, these drugs are effective only in a proportion of RA patients. Hence, there is a need to search for new therapeutic agents that are effective yet safe. Interestingly, a variety of herbs and other natural products offer a vast resource for such anti-arthritic agents. We discuss here the basic features of RA pathogenesis; the commonly used animal models of RA; the mainstream drugs used for RA; the use of well-characterized natural products possessing anti-arthritic activity; the application of nanoparticles for efficient delivery of such products; and the interplay between dietary products and the host microbiome for maintenance of health and disease induction. We believe that with several advances in the past decade in the characterization and functional studies of natural products, the stage is set for widespread clinical testing and/or use of these products for the treatment of RA and other diseases.

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The Micro-RNA Expression Profiles of Autoimmune Arthritis Reveal Novel Biomarkers of the Disease and Therapeutic Response

Cited by 32 publications

References 81 publications

The effect of Jiedu Huoxue decoction on rat model of experimental nonbacterial prostatitis via regulation of miRNAs

The effect of Jiedu Huoxue decoction on rat model of experimental nonbacterial prostatitis via regulation of miRNAs

The Interplay between Transcriptional Factors and MicroRNAs as an Important Factor for Th17/Treg Balance in RA Patients

Natural Products for the Treatment of Autoimmune Arthritis: Their Mechanisms of Action, Targeted Delivery, and Interplay with the Host Microbiome

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