The Microbiological Assay of Riboflavin and Nicotinic Acid in Urine

Fitzpatrick, Joseph; Tompsett, S. L.

doi:10.1136/jcp.2.2.121

Cited by 2 publications

(2 citation statements)

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“…As a nutraceutical additive, folic acid is also routinely measured in food and biological samples. There is a need to monitor its concentration in fortified foods in compliance with government regulations and product monitoring. , The standard microbiological assays commonly used for routine analysis of folic acid are based on light-scattering dispersion of the flocculent growth of the organisms Lactobacillus helveticus and Streptococcus faecalis in a folate-enriched media. − However, these are not sensitive to various chemical forms of the vitamin . Others have used a wide range of analytical techniques, including polarographic methods, which is the official method used in Russian pharmacopoeia .…”

Section: Introductionmentioning

confidence: 99%

“…58,59 The standard microbiological assays commonly used for routine analysis of folic acid are based on light-scattering dispersion of the flocculent growth of the organisms Lactobacillus helveticus and Streptococcus faecalis in a folate-enriched media. [60][61][62][63] However, these are not sensitive to various chemical forms of the vitamin. 64 Others have used a wide range of analytical techniques, including polarographic methods, which is the official method used in Russian pharmacopoeia.…”

Section: ' Introductionmentioning

confidence: 99%

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Electropolymerized Molecularly Imprinted Polymer Films of a Bis-Terthiophene Dendron: Folic Acid Quartz Crystal Microbalance Sensing

Apodaca

Pernites

Ponnapati

et al. 2010

ACS Appl. Mater. Interfaces

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A folic acid sensor was prepared via an electropolymerized molecularly imprinted polymer (E-MIP) film of a bis-terthiophene dendron on a quartz crystal microbalance (QCM). The cyclic voltammetry (CV) electrodeposition of the imprinted polymer film was monitored by electrochemical QCM or E-QCM, enabling in situ monitoring and characterization of E-MIP film formation and the viscoelastic behavior of the film. A key component of the E-MIP process is the use of a bifunctional monomer design to precomplex with the template and function as a cross-linker. The complex was electropolymerized and cross-linked by CV to form a polythiophene matrix. Stable cavities were formed that specifically fit the size and shape of the folic acid template. The same substrate surface was used for folic acid sensing. The predicted geometry of the 1:2 folic acid/terthiophene complex was obtained through semiempirical AM1 quantum calculations. The analytical performance, expressed through the figures of merit, of the sensor in aqueous solutions of the analyte was investigated. A relatively good linearity, R(2) = 0.985, was obtained within the concentration range 0-100 μM folic acid. The detection limit was found to be equal to 15.4 μM (6.8 μg). The relative cross selectivity of the folic acid imprinted polymer against the three molecules follows this trend: pteroic acid (= 50%) > caffeine (= 41%) > theophylline (= 6%). The potential and limitations of the E-MIP method were also discussed.

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