The micronucleus test in piscine erythrocytes

Udroiu, Ion

doi:10.1016/j.aquatox.2006.06.013

Cited by 212 publications

(132 citation statements)

References 24 publications

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“…At the end of 24-and 96-h exposure periods, fish blood samples were collected and MNi and NAs were scored/determined using the MN test. The piscine MN test has been successfully used to evaluate the genotoxic potential of a wide variety of chemical and physical agents; it is even indicated for conditions of chronic exposure in situ (7).…”

Section: Resultsmentioning

confidence: 99%

“…The in vitro and in vivo (tested in several bioindicator species, such as molluscs, plants, amphibians, reptiles, birds, and mammals) micronucleus assays (MN) are relatively simple, validated, reliable, and sensitive tests (7,8) standardised by the guidelines of international organisations such as the OECD (9). The MN test has been used to evaluate the effects of mutagenic compounds and detect clastogenic (agents which break chromosomes) and aneugenic agents (that induce aneuploidy or abnormal chromosomal segregation) in the cytoplasm of interphase cells in different environmental and ecotoxicological studies (7,10). An increase in the frequency of micronucleated (MNed) immature erythrocytes is an indication of induced structural or numerical chromosomal and/or the mitotic apparatus aberrations/damage in treated animals.…”

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confidence: 99%

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Genotoxicity of sub-lethal di-n-butyl phthalate (DBP) in Nile tilapia (Oreochromis niloticus) / Genotoksičnost subletalne koncentracije di-n-butil ftalata (DBP-a) u nilskoj tilapiji (Oreochromis niloticus)

Benlı

Erkmen

Erkoç

2016

Archives of Industrial Hygiene and Toxicology

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This study aimed to assess genotoxicity in Nile tilapia (Oreochromis niloticus) erythrocytes after exposure to a sub-lethal concentration of 10 mg L -1 di-n-butyl phthalate (DBP) for 24 and 96 h. The results showed that mean MN frequencies in both DBP and ethyl methane-sulfonate (EMS, positive control for MN bioassay) groups were significantly different (p<0.01) with respect to control and solvent control groups, in both exposure scenarios. When analysing nuclear abnormalities, the frequency of notched nuclei was significantly different (p<0.05) but the frequencies of other subtypes did not change. The 96-h exposure led to an increase in the mean frequencies of notched nuclei, and also caused significant differences between MN frequencies in all groups (p<0.01). Our findings indicate that sub-lethal DBP concentrations when tested in controlled laboratory conditions have genotoxic potential towards Nile tilapia. Further detailed studies should be done for the determination of the environmental risk assessment for aquatic life since DBP is a high risk contaminant of freshwater and marine ecosystems.

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Section: Resultsmentioning

confidence: 99%

mentioning

confidence: 99%

Genotoxicity of sub-lethal di-n-butyl phthalate (DBP) in Nile tilapia (Oreochromis niloticus) / Genotoksičnost subletalne koncentracije di-n-butil ftalata (DBP-a) u nilskoj tilapiji (Oreochromis niloticus)

Benlı

Erkmen

Erkoç

2016

Archives of Industrial Hygiene and Toxicology

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“…To that effect, micronucleus and erythrocyte nuclear abnormalities assays are frequently used for evaluation of the water pollution (Klobučar et al, 2003;Talapatra and Banerjee, 2007;Pavlica et al, 2008;Omar et al, 2012). The formation of micronuclei is the consequence of chromosomal damage or dysfunction of mitotic apparatus and represents acentral chromosomal fragments or whole chromosomes that have been excluded from the main nucleus during cell division (Udroiu, 2006). Although the mechanism of the erythrocyte nuclear abnormalities formation has not been fully explained, they have been considered as genotoxic analogues of micronuclei.…”

Section: Fish Plasma Metabolites and Enzymes Oxidative Stress Genotmentioning

confidence: 99%

Impact of treated wastewater on organismic biosensors at various levels of biological organization

Popović

Strunjak‐Perović

Klobučar

et al. 2015

Science of The Total Environment

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Relating the treated wastewater quality and its impact on organismic biosensors (Prussian carp, Carassius gibelio and earthworm, Eisenia fetida) was the main objective of the study. The impact on health status of fish living downstream, microbiological contamination and antimicrobial resistance, fish tissue structure, blood biochemistry, oxidative stress, genotoxic effects, as well as multixenobiotic resistance mechanism (MXR) was assessed. Treated wastewater discharged from the WWTP modified the environmental parameters and xenobiotic concentrations of the receiving surface waters. Potential bacterial pathogens from fish and respective waters were found in relatively low numbers, although they comprised aeromonads with a zoonotic potential. High resistance profiles were determined towards the tested antimicrobial compounds, mostly sulfamethoxazole and erytrhomycin. Histopathology primarily revealed gill lamellar fusion and reduction of interlamellar spaces of effluent fish. A significant increase in plasma values of urea, total proteins, albumins and triglycerides and a significant decrease in the activity of plasma superoxide dismutase were noted in carp from the effluent-receiving canal. Micronucleus test did not reveal significant differences between the examined groups, but a higher frequency of erythrocyte nuclear abnormalities was found in fish sampled from the effluent-receiving canal. Earthworms indicated to the presence of MXR inhibitors in water and sludge samples, thus proving as a sensitive sentinel organism for environmental pollutants. The integrative approach of this study could serve as a guiding principle in conducting evaluations of the aquatic habitat health in complex bio-monitoring studies.Response to Reviewers: The response is attached as a separate file. We request that this manuscript be considered for publication in Science of the Total Environment as an original, full paper. This manuscript has not been published and is not under consideration for publication in any other journal. All authors concur with the submission.The manuscript is a result of a comprehensive study which integrated various disciplines and research fields. The authors decided to present it as a whole rather than splitting it in two or more papers, for a better understanding of the discussed subject. We believe we have presented a potential guiding principle for conducting evaluations of the aquatic habitat health in complex bio-monitoring studies.I will be serving as the corresponding author for the manuscript. We hope that it is found to be interesting and appropriate for publication in Science of the Total Environment. Should you have any questions, please do not hesitate to contact me.Yours truly, Natalija Topic Popovic, DVM, PhD Response: Thank you for this clarification. We have taken a whole different approach and completely rewritten all sections relating to the PCA as they were indeed confusing, and created a new biplot for plasma parameters. We hope that the new text, as inserted in the respect...

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“…In addition, erythrocyte nuclear abnormalities (ENA) have been reported in finfish exposed to crude oil (Barsiene et al 2006). Micronuclei and most ENA types contain cytoplasmic chromosome fragments or intact chromosomes that failed in proper segregation during mitotic cell division (Udroiu 2006). The increased ENA frequencies are symptomatic to genotoxicity and suggest exposure to genotoxic compounds (Çavaş and Ergene-Gözükara 2005).…”

Section: Introductionmentioning

confidence: 99%

Impaired growth and erythrocyte nuclear lesions of immature Oreochromis niloticus exposed to waterborne crude oil: Persistent responses

Gunawickrama¹,

Panawala²,

Gunawickrama³

2016

Sri Lanka J. Aquat.

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Crude oil impact studies have largely been limited to marine setting and it hampers the understanding and predictability pertaining to freshwater environments following contamination episodes. Growth and erythrocyte nuclear abnormalities (ENA) were followed in freshwater fish Oreochromis niloticus experimentally exposed to crude oil (Arabian light) dispersed in water. Study comprised of a control (no crude oil in water), and two experimental groups with 5 µL/L and 25 µL/L crude oil dispersed in water (5 ppm and 25 ppm respectively), and involved continuous exposure of fish (35 fish/tank in triplicate) with static renewal over a 90-day period. Growth was estimated serially at 18-day intervals (n=40-60). Both exposure groups reduced (p<0.05) weight-based and length-based growth rates and specific growth rates. Giemsa-stained peripheral blood and head kidney smears on day 90 (n=6) showed increased (p<0.05) micronuclei, nuclear buds, fragmented apoptotic nuclei and other types of ENA frequencies (per 1000 RBC) in the fish exposed to 25 ppm crude oil. Moreover, crude oil induced ENA levels were quantitatively different (p<0.05) between the peripheral blood and head kidney. Results show that crude oil hampers growth and sustains elevated ENA of O. niloticus juveniles in freshwater environment.

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The micronucleus test in piscine erythrocytes

Cited by 212 publications

References 24 publications

Genotoxicity of sub-lethal di-n-butyl phthalate (DBP) in Nile tilapia (Oreochromis niloticus) / Genotoksičnost subletalne koncentracije di-n-butil ftalata (DBP-a) u nilskoj tilapiji (Oreochromis niloticus)

Genotoxicity of sub-lethal di-n-butyl phthalate (DBP) in Nile tilapia (Oreochromis niloticus) / Genotoksičnost subletalne koncentracije di-n-butil ftalata (DBP-a) u nilskoj tilapiji (Oreochromis niloticus)

Impact of treated wastewater on organismic biosensors at various levels of biological organization

Impaired growth and erythrocyte nuclear lesions of immature Oreochromis niloticus exposed to waterborne crude oil: Persistent responses

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