The Micronucleus Test in Urothelial Cells and Uterine Smears of Cervix Cancer Patients: A Comparison

Gandhi, Gursatej; Sharma, Pankaj; Kaur, Avneet; Badaruddoza, A.J.S.

doi:10.1080/09723757.2003.11885837

Cited by 11 publications

(20 citation statements)

References 13 publications

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“…Appli cation of molecular cytogenetic technique (FISH) supports this observation in 143 women [11]. The results obtained in Armenia (71 studied patients in total) and India (55 studied patients) are in agreement with the mentioned data -sub stantial increase in MN level in exfoliated cervix cells of cervix cancer patients [12][13][14][15][16]. Gandhi and Kaur found a correlation between the stage of cancer and MN number in exfoliated cervical cells.…”

supporting

confidence: 75%

“…Gandhi and Kaur found a correlation between the stage of cancer and MN number in exfoliated cervical cells. It is noteworthy, that they also found that along with increase of MN level in cervix cells urothelial cells MN increased [14][15][16]. Strong correlation was registered between the mentioned two parameters.…”

mentioning

confidence: 68%

“…And, hence, both HPV infection (HPV DNA) and increased MN level in cervix cells are «necessary causes» of displasia and cervical cancer. It is of extremely importance that in urothelial cells of women suffering from cancer of cervix significantly increased number of MN was also observed [16]. Based on the mentioned data, some investigators [9,10,[14][15][16] proposed that MN test in exfoliated cervix and urothelial cells should be applied in mass screening pro grams in developing countries based on high corre lation between the results in MN assay in exfoliat ed cervix cells and Pap test on one the hand, and a correlation between cancer and increased MN level in urothelial and cervix cells on the other.…”

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confidence: 99%

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Possible role of the micronucleus assay in diagnostics and secondary prevention of cervix cancer: A minireview

Nersesyan

2007

Cytol. Genet.

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Recent literature data are presented concerning micronu clei (MN) frequency in exfoliated cells of cervix cancer patients. These data strongly support a positive correlation between the MN level and malignization (changes from pre malignant stage to cancerCervical cancer is the second most common cancer in women worldwide and the leading cause of cancer mortality in women in developing coun tries [1]. In the United States, over $6 billion is spent annually in the evaluation and treatment of low grade cervical lesions, many of which do not deve lope into full blown cancer. In developing coun tries cervical cancer goes undetected because of the cost of testing and the lack of resources and trained personnel to screen and diagnose the dis ease [1]. The goal of one of the programs of the National Cancer Institute is to assess the emerging technologies of fluorescence and reflectance spec troscopy and quantitative cytology and histopathol ogy for the diagnosis of cervical cancer. All of these technologies should decrease mortality, morbidity, and the cost of treating cervical cancer.Recently Leyden et al.[2] examined factors associated with the diagnosis of cervical cancer among women enrolled in health plans and con cluded that to reduce the incidence of invasive cer vical cancer, the Papanicolaou (Pap) cervical cytology screening (which helps to reduce cervical cancer rates through the detection of premalignant lesions) should be improved. This test is of extremely importance in cancer prevention. Recent publication has shown that during first 18 months after the last negative screening Pap test in women with > or = 3 prior negative tests, cancer incidence increases to an estimated 4-5 per 100,000 woman years in each of the subsequent 2 years [3].It is well known that some specific human papil loma viruses (HPV) are associated with cancer and dysplasia of cervix, penis, anus, vagina, and vulva [4,5]. These viruses selectively infect the epitheli um of skin and mucous membranes and it results in numerical and structural chromosome aberra tions, chromosomal instability, increased aneu ploidy, and these events initiate cervical carcinogen esis. The micronucleus [fragment of chromosomes and/or whole chromosome lagged in the mytosis] assay (MN) can register both numerical and struc tural chromosomal aberrations, and MN can be easily detected in exfoliated human cells [6,7]. Since HPV infection induces cytogenetic instabil ity in cervix cells, it can be evaluated by means of MN assay. Indeed, it has been shown that in exfo liated cervical cells of patients with moderate and severe dysplasia a significantly higher frequency of MN level was observed compared with healthy women [8]. The same effect was shown by a group 64ISSN 0564-3783. Цитология и генетика.

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confidence: 75%

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confidence: 68%

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confidence: 99%

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Possible role of the micronucleus assay in diagnostics and secondary prevention of cervix cancer: A minireview

Nersesyan

2007

Cytol. Genet.

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“…This is because; the method utilizes a non-invasive process of sample collection of sample collection and also score effectively for cytogenetic damage. 22 Another study conducted by Gandhi and Kaur showed that MNT is particularly more effective in detecting evidences of cervix cancers. 23 In this case, MNT is conducted in the uterine smears of cervix cancer patients.…”

Section: Using Micronucleus In Gynecological Cancer Predictionmentioning

confidence: 99%

“…22 The two tests focused on assessment of cytogenetic damage on patients that are suffering from cervix cancer. This is practically feasible as the genetic end-point screening for micronuclei, enables researchers to measure both chromosome breakage and chromosome loss.…”

Section: Using Micronucleus In Gynecological Cancer Predictionmentioning

confidence: 99%