The microRNA.org resource: targets and expression

Betel, Doron; Wilson, Manda; Gabow, Aaron; Marks, Debora S.; Sander, Chris

doi:10.1093/nar/gkm995

Cited by 2,237 publications

(1,999 citation statements)

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“…The screen yielded miRNAs belonging to the miR‐125 and miR‐181 families, and their ability to modulate CBX7 was confirmed in a number of ways (O'Loghlen et al ., 2012). We subsequently took advantage of target prediction software ( targetscan and microRNA.org) (Betel et al ., 2008; Friedman et al ., 2009), which in addition to correctly predicting the target sites for miR‐125 and miR‐181 in CBX7 identified a potential target site for miR‐9 in the 3′UTR. Manual analysis identified a putative second target site for miR‐9 (Fig.…”

Section: Resultsmentioning

confidence: 99%

CBX7 and miR‐9 are part of an autoregulatory loop controlling p16^INK^4a

et al. 2015

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SummaryPolycomb repressive complexes (PRC1 and PRC2) are epigenetic regulators that act in coordination to influence multiple cellular processes including pluripotency, differentiation, cancer and senescence. The role of PRCs in senescence can be mostly explained by their ability to repress the INK4/ARF locus. CBX7 is one of five mammalian orthologues of Drosophila Polycomb that forms part of PRC1. Despite the relevance of CBX7 for regulating senescence and pluripotency, we have a limited understanding of how the expression of CBX7 is regulated. Here we report that the miR‐9 family of microRNAs (miRNAS) downregulates the expression of CBX7. In turn, CBX7 represses miR‐9‐1 and miR‐9‐2 as part of a regulatory negative feedback loop. The miR‐9/CBX7 feedback loop is a regulatory module contributing to induction of the cyclin‐dependent kinase inhibitor (CDKI) p16INK 4a during senescence. The ability of the miR‐9 family to regulate senescence could have implications for understanding the role of miR‐9 in cancer and aging.

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Section: Resultsmentioning

confidence: 99%

CBX7 and miR‐9 are part of an autoregulatory loop controlling p16^INK^4a

et al. 2015

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“…Databases for predicted target genes consisted of miRanda,26 RNA22,27 and Targetscan,28 as well as miRWalk 2.0 25. Putative target genes were identified when the miRNA‐target interactions were verified by three or more databases.…”

Section: Methodsmentioning

confidence: 99%

Maternal immune activation alters brain microRNA expression in mouse offspring

Sunwoo

Jeon²,

Moon

et al. 2018

Ann Clin Transl Neurol

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ObjectiveMaternal immune activation (MIA) is associated with an increased risk of autism spectrum disorder (ASD) in offspring. Herein, we investigate the altered expression of microRNAs (miRNA), and that of their target genes, in the brains of MIA mouse offspring.MethodsTo generate MIA model mice, pregnant mice were injected with polyriboinosinic:polyribocytidylic acid on embryonic day 12.5. We performed miRNA microarray and mRNA sequencing in order to determine the differential expression of miRNA and mRNA between MIA mice and controls, at 3 weeks of age. We further identified predicted target genes of dysregulated miRNAs, and miRNA‐target interactions, based on the inverse correlation of their expression levels.ResultsMice prenatally subjected to MIA exhibited behavioral abnormalities typical of ASD, such as a lack of preference for social novelty and reduced prepulse inhibition. We found 29 differentially expressed miRNAs (8 upregulated and 21 downregulated) and 758 differentially expressed mRNAs (542 upregulated and 216 downregulated) in MIA offspring compared to controls. Based on expression levels of the predicted target genes, 18 downregulated miRNAs (340 target genes) and three upregulated miRNAs (60 target genes) were found to be significantly enriched among the differentially expressed genes. miRNA and target gene interactions were most significant between mmu‐miR‐466i‐3p and Hfm1 (ATP‐dependent DNA helicase homolog), and between mmu‐miR‐877‐3p and Aqp6 (aquaporin 6).InterpretationOur results provide novel information regarding miRNA expression changes and their putative targets in the early postnatal period of brain development. Further studies will be needed to evaluate potential pathogenic roles of the dysregulated miRNAs.

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“…Here, bioinformatic analysis was performed on these mRNA and protein targets using microRNA.org: A resource for predicted microRNA targets and expression, based on the miRanda algorithm (http://www.microrna.org/microrna/home.do) [30,31] to determine if they could potentially be regulated by the miRNAs identified as of interest in this study. A large number of these mRNAs were found to contain putative binding sites for the target ACCEPTED MANUSCRIPT 9 miRNAs, including several mRNAs of which could potentially be targeted by more than one of the identified miRNAs (Table 3).…”

Section: Putative Mirna Targetsmentioning

confidence: 99%

Identification of microRNAs with a role in glucose stimulated insulin secretion by expression profiling of MIN6 cells

Hennessy

Clynes

Jeppesen

et al. 2010

Biochemical and Biophysical Research Communications

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Please cite this article as: E. Hennessy, M. Clynes, P. Jeppesen, L. O'Driscoll, Identification of microRNAs with a role in glucose stimulated insulin secretion by expression profiling of MIN6 cells, Biochemical and Biophysical Research Communications (2010), doi: 10.1016/j.bbrc.2010 This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. ACCEPTED MANUSCRIPT

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The microRNA.org resource: targets and expression

Cited by 2,237 publications

References 23 publications

CBX7 and miR‐9 are part of an autoregulatory loop controlling p16^INK^4a

CBX7 and miR‐9 are part of an autoregulatory loop controlling p16^INK^4a

Maternal immune activation alters brain microRNA expression in mouse offspring

Identification of microRNAs with a role in glucose stimulated insulin secretion by expression profiling of MIN6 cells

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The microRNA.org resource: targets and expression

Cited by 2,237 publications

References 23 publications

CBX7 and miR‐9 are part of an autoregulatory loop controlling p16INK4a

CBX7 and miR‐9 are part of an autoregulatory loop controlling p16INK4a

Maternal immune activation alters brain microRNA expression in mouse offspring

Identification of microRNAs with a role in glucose stimulated insulin secretion by expression profiling of MIN6 cells

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CBX7 and miR‐9 are part of an autoregulatory loop controlling p16^INK^4a

CBX7 and miR‐9 are part of an autoregulatory loop controlling p16^INK^4a