The Microtubule-Associated Protein END BINDING1b, Auxin, and Root Responses to Mechanical Cues

Squires, Shannon; Bisgrove, Sherryl R.

doi:10.1007/s00344-013-9333-9

Cited by 4 publications

(2 citation statements)

References 75 publications

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“…In accordance with the observed results above, we asked if the IR and PR phenotypes of Arabidopsis were regulated by the plant hormone auxin, which has been extensively studied in the context of plant responses to mechanical stimuli (Rashotte et al, 2000;Squires and Bisgrove, 2013;Lee et al, 2020). To address this question, we analyzed auxin-mediated root responses through the visualization of the expression patterns of the auxin-responsive marker DR5::GFP.…”

Section: Mechanical Impedance Alters Auxin Responsiveness and The Exp...mentioning

confidence: 64%

ROOT PENETRATION INDEX 3, a major quantitative trait locus associated with root system penetrability in Arabidopsis

Bello-Bello

Cambron

Ramírez

et al. 2022

Journal of Experimental Botany

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Soil mechanical impedance precludes root penetration, confining root system development to shallow soil horizons where mobile nutrients are scarce. Using a two-phase-agar system, we characterized Arabidopsis thaliana responses to low and high mechanical impedance at three root penetration stages. We found that seedlings whose roots fail to penetrate agar barriers show a significant reduction in leaf area, root length and elongation zone and an increment in root diameter, while those capable of penetrating show only minor morphological effects. Analyses using different auxin-responsive reporter lines, exogenous auxins and inhibitor treatments suggest that auxin responsiveness and PIN-mediated auxin distribution play an important role in regulating root responses to mechanical impedance. The assessment of 21 Arabidopsis accessions revealed that primary root penetrability (PRP) varies widely among accessions. To search for quantitative trait loci (QTLs) associated to root system penetrability, we evaluated a recombinant inbred population (RIL) derived from Landsberg erecta (Ler-0, with a high PRP) and Shahdara (Sha, with a low PRP) accessions. QTL analysis revealed a major-effect QTL localized in chromosome 3 (q-RPI3), which accounted for 29.98% (LOD = 8.82) of the total phenotypic variation. Employing an introgression line (IL-321), with a homozygous q-RPI3 region from Sha in the Ler-0 genetic background, we demonstrated that q-RPI3 plays a crucial role in root penetrability. This multiscale study revels new insights into root plasticity during the penetration process in hard agar layers, natural variation and genetic architecture behind primary root penetrability in Arabidopsis.

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Section: Mechanical Impedance Alters Auxin Responsiveness and The Exp...mentioning

confidence: 64%

ROOT PENETRATION INDEX 3, a major quantitative trait locus associated with root system penetrability in Arabidopsis

Bello-Bello

Cambron

Ramírez

et al. 2022

Journal of Experimental Botany

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“…And the phenotype of the triple null mutant of the three EB1 genes includes defective spindle or phragmoplast organization [11]. In addition, EB1 mutants have mild defects in gravity and touch sensing in roots [62] [63].…”

Section: Roles Of Microtubule and Eb1 In Cell Cyclementioning

confidence: 99%

The Evolution of Microtubule End-Binding Protein 1 (EB1) and Roles in Regulating Microtubule Behavior

Liu¹,

Han²

2015

AJPS

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All organisms must transmit genetic information to offspring through cell division, and mitotic spindle participates in the process. Spindle dynamics through depolymerization or polymerization of microtubules generates the driving force required for chromosome movements in mitosis. To date, studies have shown that microtubule arrays control the directions of cell division and diverse microtubule-associated proteins regulate cell division. But a clear picture of how microtubules and microtubule-associated proteins modulate cell division remains unknown. Depletion of end-binding protein 1 by RNA-mediated inhibition shows that one of the microtubule-associated proteins, end-binding protein 1, plays a crucial role in mitotic spindle formation and promotes microtubule dynamics and is needed for the proper segregation of mitotic chromosomes during anaphase in Drosophila cells. Here, we review the properties of end-binding protein 1 and the roles of end-binding protein 1 in regulating microtubule behavior and in cell cycle. 2115 namic properties of microtubules. Microtubules (MTs) are dynamic hollow tubes comprising α, β-tubulin dimers that disassemble and reassemble at two ends: the slow-growing (minus) and fast-growing (plus) ends. It is now widely known that MT behavior is modulated by a number of MT-associated proteins (MAPs), which can influence dynamic instability parameters and consequently impact on mitotic progression and fidelity. Many of these MAPs share the ability to recognize only the distal part of a polymerizing MT, known as the MT plus end. For this reason, these MAPs are currently known as MT plus-end-tracking proteins (+TIPs) [1] [2]. Recently, +TIPs have emerged as regulators of MT dynamics. The plus end explores the cell periphery and shows dynamic instability, switching rapidly between the two phases of growth and shrinkage. Thus this dynamic scaffold performs a variety of very different functions. Genetic and biochemical studies have shown that +TIPs interact with each other and form protein complexes [3].End-binding protein 1 (EB1) promotes MT polymerization and interacts directly with many other +TIPs and cytoskeletal proteins such as cytoplasmic linker protein 170 (CLIP-170) and the dynactin large subunit p150 Glued , and mitotic centromere-associated kinesin, microtubule-actin crosslinking factor and adenomatous polyposis coli (APC) [4] [5]. Thus, EB1 has been proposed to form the core of the microtubule plus-end complex and act as a hub in interactions with +TIPs [6] [7].Division of one cell into two genetically identical daughter cells occurs through two coordinated processes which are known as mitosis (division of the nucleus) and cytokinesis (division of the cytoplasm). The transition from interphase to mitosis involves a dramatic reorganization of the MT cytoskeleton. In fact, there is an increase in MT dynamics which occurs concomitantly with NEB that could be important for spindle morphogenesis [8] [9]. Studies have shown that accurate segregation of the replicated genome during cell...

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Microtubule dynamics is required for root elongation growth under osmotic stress in Arabidopsis

et al. 2014

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The Microtubule-Associated Protein END BINDING1b, Auxin, and Root Responses to Mechanical Cues

Cited by 4 publications

References 75 publications

ROOT PENETRATION INDEX 3, a major quantitative trait locus associated with root system penetrability in Arabidopsis

ROOT PENETRATION INDEX 3, a major quantitative trait locus associated with root system penetrability in Arabidopsis

The Evolution of Microtubule End-Binding Protein 1 (EB1) and Roles in Regulating Microtubule Behavior

Microtubule dynamics is required for root elongation growth under osmotic stress in Arabidopsis

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