The midgut epithelium of aquatic arthropods: a critical target organ in environmental toxicology.

Beaty, Barry J.; Mackie, Ryan S.; Mattingly, K.S.; Carlson, Jonathan O.; Rayms-Keller, Alfredo

doi:10.1289/ehp.02110s6911

Cited by 20 publications

(12 citation statements)

References 46 publications

(85 reference statements)

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“…Copper inflicted high mortality rates (94.6%) at 10 ppm after 96 hours among all three species. The high mortality rate may have been due to the large number of copper molecules that bound with receptors in the larval midgut, because copper was previously shown to damage the perithropic matrix in the midgut (Beaty et al, 2002). A copper concentration of 3.3 ppm killed half of the larvae, but did not achieve an adequate mortality rate after 96 hours.…”

Section: Discussionmentioning

confidence: 99%

Larvicidal and ovipositional preference test of copper solution for mosquitoes

Reza

Yamamoto

Matsuoka

2014

Jap. J. Sanit. Zool.

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In the previous studies, we demonstrated that larvae of Anopheles stephensi died within two weeks after the exposure of the larvae to 2.4 ppm of copper solution. The ability of copper to kill mosquito larvae prompted us to examine its applicability to ovitraps. We demonstrated in this study that 10 ppm of copper killed more than 90% of larvae (Anopheles stephensi, Aedes albopictus and Culex pipiens pallens) in 96 hours. At 3.3 ppm, Cx. pipiens was more sensitive than An. stephensi and Ae. albopictus. Fifty percent of Cx. pipiens larvae died within 24 hours but An. stephensi and Ae. albopictus survived more than 50% at 48 hours. We also observed that gravid mosquitoes of An. stephensi, Ae. albopictus, and field-collected An. sinensis did not detect nor avoid 10 ppm copper solution for laying eggs under laboratory conditions. These results suggest that copper could be used in ovitraps as a low cost alternative and the eradication of mosquito-borne diseases.

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Section: Discussionmentioning

confidence: 99%

Larvicidal and ovipositional preference test of copper solution for mosquitoes

Reza

Yamamoto

Matsuoka

2014

Jap. J. Sanit. Zool.

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“…Some studies have revealed that copper has larvicidal capabilities. The peritrophic matrix of aquatic arthropods was perturbed by copper (Beaty et al, 2002). Among eggs of Aedes aegypti (Linnaeus, 1762), 100 failed to hatch when exposed to 32 ppm of copper, and an LD 50 of larvae in 24 hours was achieved with 33 ppm (Keller et al, 1998).…”

Section: Introductionmentioning

confidence: 99%

Low-concentration copper solution jeopardizes larval movement and ability to survive predation: New insight into malaria eradication via vector control

Reza

Yamamoto

Matsuoka

2012

Jap. J. Sanit. Zool.

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A new approach to eradicating malaria is urgently required. Emphasizing vector eradication via larval control, we tried to find a safe concentration of copper, which can be utilized to reduce larval survival. It has been observed that mosquito larvae died when exposed to solid copper in water. We confirmed this phenomenon in our own experiments. We next studied the minimum copper concentration for effectively killing or disturbing Anopheles stephensi larvae. A concentration of 0.6 ppm had a significant impact on larval movement, making them vulnerable to predatory fish. Feeding time tests showed that guppies (Poecilia reticulata) ate the weakened larvae significantly faster than control larvae. Utilizing low concentrations of copper together with predatory fish in the field may shed new light on malaria eradication via vector control.

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“…Notably, some MRGs found in previous studies with Diptera such as AEIMUCI (Ae. aegypti intestinal mucin) and CTTUBI (Chiromomus tentans ␣-tubulin) were not identiÞed in this study (Beaty et al 2002). Sublethal concentrations of toxic metals, such as copper and calmium, induced expression of these two proteins, which could enhance survivorship of aquatic arthropods in polluted environments.…”

Section: Discussionmentioning

confidence: 78%

“…Metal-induced gene expression in the mosquito midgut has been previously documented: exposing Aedes aegypti (L.) larvae to heavy metals showed AEIMUC1 (Ae. aegypti intestinal mucin) to be a metal-responsive gene, MRG (Rayms-Keller et al 2000), and molecular biological studies have identiÞed midgut genes induced by heavy metals in the midgut epithelium of aquatic arthropods (Beaty et al 2002).…”

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confidence: 99%

Identification and Expression Profile of Multiple Genes in Response to Magnesium Exposure in Culex quinquefasciatus Larvae

Zhao¹,

Becnel²,

Clark³

et al. 2010

jnl. med. entom.

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Magnesium is crucial for baculovirus transmission in Culex nigripalpus (Theobald) and Culex quinquefasciatus (Say) larvae. However, the mechanistic role of magnesium in baculovirus transmission is unknown. To investigate the possible role of host response factors in baculovirus transmission, suppression subtractive hybridization was used to identify genes differentially transcribed after magnesium exposure in Cx. quinquefasciatus larvae. Suppression subtractive hybridization was performed in both directions enriching for cDNAs differentially transcribed between a nonmagnesium larval control and magnesium (15 mM MgSO4) treatment of Cx. quinquefasciatus larvae held for 1 h at 27 degrees C. Clones from differentially transcribed genes were evaluated by sequencing, and relative gene transcription levels were analyzed using quantitative real-time polymerase chain reaction quantitative real-time polymerase chain reaction. Target transcripts up/downregulated by magnesium included Cx. quinquefasciatus troponin C, isocitrate dehydrogenase, allergen, cytochrome b5, chymotrypsinogen, apolipophorins, tryptase gamma, carboxylesterase, prolylcarboxypeptidase, imaginal disc growth factor, aldehyde dehydrogenase, tropomyosin-1, chitotriosidase, heat shock protein 70 B2, inorganic phosphate cotransporter, and many other hypothetical protein genes. Magnesium can alter gene transcription in a vector mosquito population, and understanding this process can provide insight into the mechanistic role of magnesium in baculovirus transmission.

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The midgut epithelium of aquatic arthropods: a critical target organ in environmental toxicology.

Cited by 20 publications

References 46 publications

Larvicidal and ovipositional preference test of copper solution for mosquitoes

Larvicidal and ovipositional preference test of copper solution for mosquitoes

Low-concentration copper solution jeopardizes larval movement and ability to survive predation: New insight into malaria eradication via vector control

Identification and Expression Profile of Multiple Genes in Response to Magnesium Exposure in Culex quinquefasciatus Larvae

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