The migration of primordial germ cells in the chick embryo

Meyer, David

doi:10.1016/0012-1606(64)90009-0

Cited by 177 publications

(83 citation statements)

References 35 publications

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“…Some isolated cells in the dorsal mesentery were strongly labelled by the c-kit probe. They were found as rostrally as the cervical region (somite 8 to 15) and were, therefore, unlikely to have been migrating germ cells (Meyer, 1964). They may have been hemopoietic cells similar to those found in the ventral wall of the aorta and in the dorsal mesentery of E3 and E4 chick embryos (Dieterlen-Li&vre and Martin, 1981).…”

Section: C-kit and S Z Expression During Neural Crest Cell Migrationmentioning

confidence: 94%

Steel and c‐kit in the development of avian melanocytes: A study of normally pigmented birds and of the hyperpigmented mutant silky fowl

Lecoin

Lahav

Martin

et al. 1995

Developmental Dynamics

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We describe here the expression of c-kit and Steel (SZ) genes during the development of melanocytes in normally pigmented strains of chick and quail compared to unpigmented (White Leghorn) and hyperpigmented (Silky Fowl) strains of chickens. By using the quailkhick chimera system, we found that the neural crest cells, which migrate dorso-laterally in the subectodermal mesenchyme to give rise to the melanocytes, express c-kit as early as E4, that is about 2 days after they have left the neural primordium. The SZ gene is expressed from E4 onward in the epidermis but not at all in the dermis at any developmental stage. As feather buds develop, S Z mRNA becomes restricted to the apical region of the feather filaments. During formation of the barbs and barbules of the down feather, production of the Steel factor is restricted to the external epidermal cells of the barbules. The cell bodies of the c-kit-positive melanocytes are then located in the internal border of the epidermal ridges and extend their processes toward the source of the Steel factor. We propose that the spa-

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Section: C-kit and S Z Expression During Neural Crest Cell Migrationmentioning

confidence: 94%

Steel and c‐kit in the development of avian melanocytes: A study of normally pigmented birds and of the hyperpigmented mutant silky fowl

Lecoin

Lahav

Martin

et al. 1995

Developmental Dynamics

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“…However, the embryological origin of the mesenchymal moiety and its possible filiation with the mesonephric blastema (Witschi, 1951) remained unsolved and needed to be studied at earlier stages. As gonad formation starts as early as stage 14 in chick and quail embryos (Meyer, 1964 ;Didier and Fargeix, 1976), we re-examined the development of the early gonad by light and transmission electron microscopy, studying sequential morphogenetic events and the ultrastructural characteristics of cell populations between days 2 and 5 of incubation, when the PGC's settle into forming gonads and begin to proliferate. The observation of morphological relationships between somatic and germ cells was facilitated by specific labeling of the glycogen in the germ cells.…”

mentioning

confidence: 99%

Early sequential development in avian gonads. An ultrastructural study using selective glycogen labeling in the germ cells

Fargeix¹,

Didier²,

Didier³

1981

Reprod. Nutr. Dévelop.

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“…The PGCs were cultured for five weeks and treated for subculture on an average of three days. The PGCs cell morphology and growth characteristics are the basis identification of PGCs (Meyer 1964). The size of PGCs, large amounts of glycogen granules in the cytoplasm and large nuclei are bigger than somatic cells.…”

Section: Culture and Characterization Of Chicken Pgcsmentioning

confidence: 99%

Cultivation and Biological Characterization of Chicken Primordial Germ Cells

Meng

Guan

Gao

et al. 2016

Braz. arch. biol. technol.

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The purpose of this work was to investigate the isolation, culture process of chicken gonadal primordial germ cells (PGCs) and study their biological characterization. PGCs were harvested from 5.5-day-old chicken embryonic genital ridges and explanted onto chicken embryonic fibroblasts (CEFs). The results showed that the primary cultivation of chicken PGCs on their own gonadal stroma cells were better than CEFs at first two days for reproduction. The conditioned media supported the growth and colony formation of PGCs for a prolonged time in vitro and maintained a normal diploid karyotype, which were positively stained by alkaline phosphatase (AKP), periodic acid Schiff (PAS)and reacted with anti-SSEA-1, Oct4, showed that they expressed the stage specific genes CVH, Blimp1 and Dazl, the stem cell specific genes Sox2, Pouv and Nanog. They also formed the embryoid bodies (EBs). These results suggested that the chicken PGCs cultured in vitro not only had strong selfrenewal ability, but also had the potential capability of multi-lineage differentiation.

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The migration of primordial germ cells in the chick embryo

Cited by 177 publications

References 35 publications

Steel and c‐kit in the development of avian melanocytes: A study of normally pigmented birds and of the hyperpigmented mutant silky fowl

Steel and c‐kit in the development of avian melanocytes: A study of normally pigmented birds and of the hyperpigmented mutant silky fowl

Early sequential development in avian gonads. An ultrastructural study using selective glycogen labeling in the germ cells

Cultivation and Biological Characterization of Chicken Primordial Germ Cells

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