The migration of recirculating autologous and allogeneic lymphocytes through single lymph nodes

Frost, Heiner; Cahill, Ross N. P.; Trnka, Z.

doi:10.1002/eji.1830051208

Cited by 51 publications

(62 citation statements)

References 20 publications

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“…By flow cytometry, 3 h after injection of labeled cells, 0.05 Ϯ 0.01% of the injected cells were recovered per 10 9 lymph node cells, but no labeled cells could be detected in the efferent lymph. Previous experiments have clearly demonstrated that the recirculation kinetics of labeled lymphocytes into afferent lymph parallels that seen in efferent lymph, and that virtually no labeled cells can be detected entering the lymph node via afferent lymph at this early time point (28,29). However, using flow cytometry, sufficient numbers of labeled cells could be clearly differentiated within the lymph node and phenotyped for conventional T and B cell subsets (Fig.…”

Section: Cd4 ϩ and ␥␦-Tcr ϩ T Cells Home To Sclns In Similar Numbersmentioning

confidence: 64%

Subset-Specific Regulation of the Lymphatic Exit of Recirculating Lymphocytes In Vivo

Young¹,

Marston²,

Dudler³

2000

The Journal of Immunology

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The blood-to-lymph recirculation of lymphocytes is required for the maintenance of immune surveillance and the dissemination of memory. Although the ability of lymph-borne cells to recirculate has been well documented, relatively less is known about the migration capacity of PBLs. We have found a clear preference for PBLs to recirculate through s.c. rather than intestinal lymph nodes. This preference could be directly attributed to the migratory characteristics of γδ-T cells. γδ-T cells were found to express significantly higher levels of L-selectin than other subsets, suggesting that at least some of this preferential migration could be attributed to their interaction with ligands on vascular endothelium. More detailed experiments showed that γδ-T cells migrated through lymph nodes with greater efficiency than αβ T cells or B cells, which clearly indicated an enhanced ability of γδ-T cells to exit lymph nodes in the efferent lymph independent of entry from the blood. This hypothesis was supported by histological examination, where γδ-T cells were found almost exclusively in the interfollicular traffic areas within lymph nodes. These data indicate that γδ-T cells are the most active recirculating lymphocyte subset in ruminants and suggest new mechanisms to regulate the traffic of lymphocyte subsets through normal lymph nodes.

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Section: Cd4 ϩ and ␥␦-Tcr ϩ T Cells Home To Sclns In Similar Numbersmentioning

confidence: 64%

Subset-Specific Regulation of the Lymphatic Exit of Recirculating Lymphocytes In Vivo

Young¹,

Marston²,

Dudler³

2000

The Journal of Immunology

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“…Allogeneic T cells from postnatal lambs were used in our experiments because of the unavailability of inbred sheep. We felt justified in using allogeneic T cells because, unlike allogeneic T cells in adult sheep which do not recirculate to any extent, 14 the recovery of labelled allogeneic T cells in fetal lymph and lymph nodes is similar to autologous cells. This indicates that large numbers of allogeneic T cells are able to recirculate between blood and lymph in the fetus.…”

Section: Discussionmentioning

confidence: 99%

Late‐term fetal thymectomy does not prevent the development of gut‐homing T cells after birth

et al. 1996

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SUMMARYTissue-specific circulation of T cells is a critical element in the integration of systemic immune responses. Current models of T-cell migration suggest that homing specificities of T cells for tissues such as gut and skin are generated outside the thymus as a result of activation of virgin T cells by antigen in lymph nodes. We have used the sheep fetus (which is immunologically virgin and contains no memory or effector T-cell subsets) to examine the migration of 51 Cr-labelled T cells in vivo. We report that gut-homing T cells are not present in the fetus and that gut-homing T cells from postnatal lambs home normally to fetal gut. Fetal thymectomy performed immediately prior to birth failed to prevent the development of gut-homing T cells in postnatal life. Gut-homing specificities on T cells are thus acquired extrathymically.

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“…It has been demonstrated that the majority of cells in the afferent lymph vessels do not transit across into efferent lymph but remained in the lymph node 18 . In contrast, only a small fraction of the cells entering the efferent vessel are derived from the afferent lymphatic vessels or produced by multiplication within the node [19][20][21] . If this small fraction is required, the assumption that an LTI-SISO system is appropriate for studying peripheral lymphocyte migration becomes more reasonable.…”

Section: Introductionmentioning

confidence: 99%

An application of linear output error modelling for studying lymphocyte migration in peripheral lymphoid tissues

Srikusalanukul

Bruyne

McCullagh³

2002

Australas. Phys. Eng. Sci. Med.

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Lymphocyte recirculation between lymphatic and blood vessels and migration through tissues are essential mechanisms underlying immunological surveillance. However, the kinetics of lymphocyte migration through lymphoid tissues remains poorly understood. The present study of lymphocyte migration, based on a sheep model and entailing the long term cannulation of blood vessels and lymphatic vessels efferent from lymph nodes, represents the first attempt to apply control engineering based models to overcome some of the experimental impediments to understanding the complex phenomena involved in lymphocyte migration. An output error model order (1,2,nk) was systematically selected under given criteria from four classes of Linear Time-Invariant Single-Input Single-Output, (LTI-SISO) systems to represent the peripheral lymph node system. The unit impulse responses were simulated under noise free conditions and their features were extracted to describe the dynamics of the system. The findings from this study revealed novel information about several aspects of the dynamics of lymphocyte migration.

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The migration of recirculating autologous and allogeneic lymphocytes through single lymph nodes

Cited by 51 publications

References 20 publications

Subset-Specific Regulation of the Lymphatic Exit of Recirculating Lymphocytes In Vivo

Subset-Specific Regulation of the Lymphatic Exit of Recirculating Lymphocytes In Vivo

Late‐term fetal thymectomy does not prevent the development of gut‐homing T cells after birth

An application of linear output error modelling for studying lymphocyte migration in peripheral lymphoid tissues

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