The mitochondrial genome of the Vespa mandarinia Smith (Hymenoptera: Vespidae: Vespinae) and a phylogenetic analysis of the Vespoidea

Abstract

We report the mitochondrial genome of the Vespa mandarinia Smith (GenBank accession no. KR059904) for the subfamily Vespinae (Hymenoptera: Vespidae). This is the first completely sequenced mitochodrial genome from the genus Vespa. All of the typical mitochondrial genes were sequenced with a length of 15 902 bp. In the V. mandarinia mitochondrial genome, four rearrangement events occurred compared with the pupative ancestral arrangement of insects, corresponding to the shuffling of trnQ and trnM, translocation of trnY to the region between A + T-rich region and trnI-trnM-trnQ, shuffling of trnS1 and trnE in the tRNA cluster of trnA-trnR-trnN-trnS1-trnE-trnF, and translocation of trnL1 from a location between nad1 and rrnL to the upstream of nad1 gene. Phylogenetic analysis using the Bayesian method based on all codon positions of the 13 protein-coding genes supports the monophyly of Vespidae and Formicidae. Within the Formicidae, the Myrmicinae and Dolichoderinae form a sister group, while within the Vespidae, the Eumeninae sister to lineage of Vespinae + Polistinae. Our study showed that the mitochondrial genomes between Vespinae and Polistinae shared more arrangement patterns than that between Vespinae and Eumeninae.

Complete mitochondrial genome of the black‐tailed hornet, Vespa ducalis (Hymenoptera: Vespidae): Genomic comparisons in Vespoidea

“…With regard to Vespidae, a strong sister relationship between Vespinae and Polistinae (BPP = 1; ML, 100 %) was obtained, with the placement of Eumeninae as the basal lineage. Consistent results for these relationships have previously been reported (Chen et al ; Wei et al ). Within Vespa , the sister relationship between V. ducalis and V. mandarinia —leaving V. bicolor as sister to the other two Vespa species—was strongly supported (BPP = 1; ML, 94 %).…”

Next-Generation Sequencing of Two Mitochondrial Genomes from Family Pompilidae (Hymenoptera: Vespoidea) Reveal Novel Patterns of Gene Arrangement

“…The other ten pairs of genes were directly adjacent to each other. In both species, the length of the mitochondrial genome and overlapping regions between genes was normal, while the intergenic spacer is considerably longer than other Vespoidea mitochondrial genomes [22,34,35,36]. …”

“…Currently, nearly complete mitochondrial genomes from Vespidae, Eumenidae, Formicidae and Mutillidae have been sequenced respectively within Vespoidea. In the sequenced mitochondrial genomes of Vespoidea, tRNA gene rearrangement was reported; however, rearrangement of protein-coding genes has not been found [22,34,35,36]. A locus of mitochondrial genes comprising cox1 and adjacent tRNA genes was sequenced from representatives of the major clades of Pompilidae and the rearrangement of the trnC and trnY genes was found in the sequenced segments [37].…”

“…However, no complete mitochondrial genome from Pompilidae has been reported. We presumed a moderate amount of mitochondrial gene rearrangement occurred in species of Pompilidae according to current knowledge of its related families from Vespoidea [22,34,35,36], but the contribution of the rearrangement signal to phylogenetic analysis of Hymenoptera still needs confirmation.…”

Complete mitochondrial DNA sequence of the invasive hornetVespa velutina(Insecta, Hymenoptera) found in Japan

“…Of the 13 proteincoding genes, the initiation codons ATC was found in one, ATG in four, ATT in five, and ATA in three genes, while TAA was the termination codon in all these genes. Phylogenetic analysis using the 13 mitochondrial protein-coding genes from seven closely related taxa of Vespidae (Cameron et al 2008;Song et al 2016;Wei et al 2016;Zhou et al 2016) suggested a sister relationship between V. velutina and V. bicolor (Archer 2012;Chen et al 2016).…”