The mitochondrial genome of the Mediterranean fruit fly, <i>Ceratitis capitata</i>

Spanos, Lefteris; Koutroumbas, G.; Kotsyfakis, Michail; Louis, Christos

doi:10.1046/j.1365-2583.2000.00165.x

Cited by 121 publications

(81 citation statements)

References 33 publications

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“…Given its maternal inheritance, small size, lack of recombination and presence of polymorphisms, the mtDNA proved to be a valuable tool to study the various patterns existing in many insect species such as Drosophila (Lewis et al 1995), Phlebotomus (Esseghir et al 1997) and Ceratitis capitata populations (Spanos et al 2000). Initial studies on the medfl y genetic diversity were based on a RFLP-PCR-based approach using the ND5-tRNA-ND4 mitochondrial locus to detect the haplotype variants.…”

Section: Discussionmentioning

confidence: 99%

“…In conclusion, the direct analysis of mitochondrial DNA sequences from the ND5 region has uncovered a rich array of distinct haplotypes within populations of the Mediterranean fruit fl y. Th is approach makes it possible to use sophisticated tools for the analysis of networks of intraspecifi c gene genealogies (Posada & Crandall 2001) without sacrifi cing the traditional advantages of using mitochondrial DNA (Steck et al 1996;Spanos et al 2000) to analyze populations of this and other pest species. Furthermore, we have shown that our approach allows for the detailed analysis of highly variable populations, such as those from countries like Tunisia, which had proven to be refractive to analysis using the PCR-RFLP method.…”

Section: Discussionmentioning

confidence: 99%

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Genetic Diversity of ND5 mitochondrial patterns inCeratitis capitata(Diptera: Tephritidae) populations from Tunisia

Elfékih

Makni

Haymer

2010

Annales de la Société entomologique de France (N.S.)

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Abstract. The Mediterranean fruit fl y (medfl y) Ceratitis capitata (Diptera: Tephritidae) is known to be one of the most destructive and economically important agricultural pests worldwide. Several previous research projects have investigated the genetic makeup of regional populations of this pest and the relationships of populations from different areas of the world, including countries from the Mediterranean region. However, previously, little information has been reported on populations from Tunisia, despite the fact that this pest occurs in several agriculturally sensitive areas of this country. In order to study the genetic diversity of medfl y populations within Tunisia, specimens were collected from the Coastal, Northern, Central and Southern regions of the country. Results using mitochondrial ND5 sequences show the presence of distinct haplotypes. This data used to analyze the levels of genetic variability within and between populations from Tunisia as well as from other countries in the Mediterranean region (Morocco and Israel) and in the world (Seychelles and Hawaii). This study also leads to a better understanding of the origin of new infestations and the colonization processes involving this pest. Résumé. Diversité génétique des confi gurations mitochondrial ND5 chez les populations deCeratitis capitata (Diptera : Tephritidae) de Tunisie. La mouche méditerranéenne des fruits Ceratitis capitata (Wiedemann) (Diptera : Tephritidae) est l'un des ravageurs les plus importants en agriculture dans le monde. Plusieurs travaux de recherche ont étudié les caractéristiques génétiques de la cératite ainsi que les relations entre populations de ce ravageur provenant de différentes régions du globe y compris les pays appartenant au bassin méditerranéen. Cependant, très peu d'informations sont disponibles concernant les populations de cératite en Tunisie, malgré l'importance économique de ce ravageur dans des zones clés de production fruitière tunisiennes. Dans le but d'étudier la diversité génétique des populations de cératite en Tunisie, des spécimens ont été collectés du Cap Bon, Nord, Centre et sud de la Tunisie. Les résultats basés sur l'étude des séquences du gène mitochondrial ND5 révèlent la présence d'haplotypes distincts. Ces haplotypes ont permis d'analyser les niveaux de variabilité génétique entre les populations tunisiennes et les autres populations de cératite du bassin méditerranéen (Maroc et Israël) ainsi que des populations prélevées dans d'autres régions du monde (Iles Seychelles et Hawaï). Cette étude a permis également de mieux caractériser l'origine de nouvelles infestations ainsi que les processus de colonisation de ce ravageur.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Genetic Diversity of ND5 mitochondrial patterns inCeratitis capitata(Diptera: Tephritidae) populations from Tunisia

Elfékih

Makni

Haymer

2010

Annales de la Société entomologique de France (N.S.)

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“…This marker, because of these characteristics, has been widely used in studies of population genetics and evolutionary relationships of many groups of organisms (Avise, 1994;Spanos et al, 2000). ND5 (coding for subunit 5 of NADH dehydrogenase) is one of the most variable genes of mtDNA and has been studied in several species of the Anopheles and Aedes genera (Besansky et al, 1997;Birungi and Munstermann, 2002;MolinaCruz et al, 2004).…”

Section: Introductionmentioning

confidence: 99%

Reduced levels of genetic variation in Aedes albopictus (Diptera: Culicidae) from Manaus, Amazonas State, Brazil, based on analysis of the mitochondrial DNA ND5 gene

Maia¹,

Scarpassa²,

Maciel-Litaiff³

et al. 2009

Genet. Mol. Res.

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ABSTRACT. Aedes albopictus, a mosquito originally from Southeast Asia, is considered to be one of the main vectors of dengue fever, yellow fever and other arboviruses. We examined the genetic variability and population structure of 68 individuals of Ae. albopictus collected from five neighborhoods of the city of Manaus, based on the mitochondrial gene coding for NADH dehydrogenase subunit 5 (ND5). Two haplotypes were found, separated by a single mutational event (T ↔ C), with extremely low levels of genetic variability (h = 0.187 ± 0.059; π = 0.00044 ± 0.00014). Based on AMOVA, we concluded that most of the variation (99.08%) occurred within populations, though the levels of variation were not significant. Neutrality tests (Tajima's D and Fu's Fs) were non-significant, indicating that these populations are in genetic equilibrium. The most frequent haplotype (H1) is restricted to Brazilian populations of Ae. albopictus, while the rarer haplotype (H2) is shared with populations from the United States and Asia. We suggest that the reduced variability and low genetic structure identified in our study is a consequence of the recent introduction of this species in Manaus, possibly through a founder effect, followed by expansion throughout the city neighborhoods. Genetic similarity would therefore be due to insufficient time to have accumulated genetic differences between the populations of Ae. albopictus and not to extensive gene flow among them.

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“…For the P. sulpitia COI gene, no typical ATN initiator was found in its starting region or in its neighboring trnY sequences. As for the cox1 initiation codon in animals, significantly different cases have been reported, for example, tetranucleotides such as TTAG in Coreana raphaelis (Kim et al, 2006), ATAA in Drosophila yakuba (Clary & Wolstenholme, 1985) are used, while hexanucleotides such as TATTAG in Ostrinia nubilalis and Ostrinia furnicalis (Coates et al, 2005), TTTTAG in Bombyx mori (Yukuhiro et al, 2002), TATCTA in Penaeus monodon (Wilson et al, 2000), ATTTAA in Anopheles gambiae (Beard et al, 1993), Anopheles quadrimaculatus (Mitchell et al, 1993), and Ceratitis capitata (Spanos et al, 2000) are used. Generally, the trinucleotide TTG was assumed to be the cox1 start codon for some invertebrate taxa including insect species, such as Pyrocoelia rufa (Bae et al, 2004), Caligula boisdnvalii (Hong et al, 2008), and Acraea issoria .…”

Section: Protein-coding Genesmentioning

confidence: 99%

Complete mitochondrial genome of the Five-dot Sergeant <I>Parathyma sulpitia</I> (Nymphalidae: Limenitidinae) and its phylogenetic implications

Tian

Sun²,

Chen³

et al. 2013

Zoological Research

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Abstract:The complete mitochondrial genome of the Parathyma sulpitia (Lepidoptera, Nymphalidae, Limenitidinae) was determined. The entire mitochondrial DNA (mtDNA) molecule was 15 268 bp in size. Its gene content and organization were the same as those of other lepidopteran species, except for the presence of the 121 bp long intergenic spacer between trnS1(AGN) and trnE. The 13 protein-coding genes (PCGs) started with the typical ATN codon, with the exception of the cox1 gene that used CGA as its initial codon. In addition, all protein-coding genes terminated at the common stop codon TAA, except the nad4 gene which used a single T as its terminating codon. All 22 tRNA genes possessed the typical clover leaf secondary structure except for trnS1(AGN), which had a simple loop with the absence of the DHU stem. Excluding the A+T-rich region, the mtDNA genome of P. sulpitia harbored 11 intergenic spacers, the longest of which was 121 bp long with the highest A+T content (100%), located between trnS1(AGN) and trnE. As in other lepidopteran species, there was an 18-bp poly-T stretch at the 3'-end of the A+T-rich region, and there were a few short microsatellite-like repeat regions without conspicuous macro-repeats in the A+T-rich region. The phylogenetic analyses of the published complete mt genomes from nine Nymphalidae species were conducted using the concatenated sequences of 13 PCGs with maximum likelihood and Bayesian inference methods. The results indicated that Limenitidinae was a sister to the Heliconiinae among the main Nymphalidae lineages in this study, strongly supporting the results of previous molecular data, while contradicting speculations based on morphological characters.

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The mitochondrial genome of the Mediterranean fruit fly, Ceratitis capitata

Cited by 121 publications

References 33 publications

Genetic Diversity of ND5 mitochondrial patterns inCeratitis capitata(Diptera: Tephritidae) populations from Tunisia

Genetic Diversity of ND5 mitochondrial patterns inCeratitis capitata(Diptera: Tephritidae) populations from Tunisia

Reduced levels of genetic variation in Aedes albopictus (Diptera: Culicidae) from Manaus, Amazonas State, Brazil, based on analysis of the mitochondrial DNA ND5 gene

Complete mitochondrial genome of the Five-dot Sergeant <I>Parathyma sulpitia</I> (Nymphalidae: Limenitidinae) and its phylogenetic implications

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