The mixed lymphocyte reaction (MLR) stimulatory capacity of human lymphocyte subpopulations

Oers, Marinus H. J. van; Zeijlemaker, W. P.

doi:10.1016/0008-8749(77)90022-3

Cited by 14 publications

(7 citation statements)

References 28 publications

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“…Previous work (8)(9)(10)(11) appearing to demonstrate the strong stimulatory capacity of the B cell in the human MLR did not utilize B cells purified sufficiently to eliminate minor populations of active contaminating cells, such as the dendritic cells. However, the new techni(lue of isolating B cells using anti-IgM-coated erythrocytes allowed isolation of B cells free of contaminating Ia-positive non-B cells.…”

Section: Discussionmentioning

confidence: 99%

“…Thus, at the stimutlator cell concentration at which normal PBNI gave mlaximnal stimulation PBM1 from these leukemic . ;subjects gener- 10 Stimulator cell concentration X 1o-6 FIGURE 1 Contrasts in NMLR stimulation generated by normal and leukemiiie PBM. The responses of PBM from one normal subject to varying numbers of PBM from another normal (-), CLL C (A), and CLL G (0) are show.,n.…”

Section: Characterizationmentioning

confidence: 99%

“…Studies (8)(9)(10)(11). Most cases of chronic lymphocytic leukemia (CLL) represent a neoplastic proliferation of B lymphocytes (12) which, like their normal counterparts, express membrane immunoglobulin (Ig) and Ia antigens (2,3,13).…”

mentioning

confidence: 99%

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Poor Mixed Lymphocyte Reaction Stimulatory Capacity of Leukemic B Cells of Chronic Lymphocytic Leukemia Patients Despite the Presence of Ia Antigens

Halper¹,

Fu²,

Gottlieb³

et al. 1979

J. Clin. Invest.

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A B S T R A C T The humana Ial-like aintigens, selectively expressed on B lymphocytes, are noow recognized to be closely associate(l with, or identical to, the genie prodlucts of the imaljor histocompatibility complex responsil)le for stimulation in the mixed lymphocyte reaction. The leuikemie B lymphocytes of patienits wvith chronie lylmphocytic leukemnia express these antigens very well. In the presenit stuidy they were readily dletected by several techniq(uies utilizing 1)oth allo-anid heteroanitiseral. However

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Section: Discussionmentioning

confidence: 99%

Section: Characterizationmentioning

confidence: 99%

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Poor Mixed Lymphocyte Reaction Stimulatory Capacity of Leukemic B Cells of Chronic Lymphocytic Leukemia Patients Despite the Presence of Ia Antigens

Halper¹,

Fu²,

Gottlieb³

et al. 1979

J. Clin. Invest.

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“…The presence of monocytes, derived either from the stimulating or responding cell population is essential for a proliferalive response to take place [29,37,38]. Monocytes have been shown to express MLCstimulating determinants [16,18,22,26,38,40] although this was not found in a recent report [33]. In the present study various cell separation techniques were investigated to define the content and yield of B ceils, T cells and monocytes in the cell fractions obtained.…”

mentioning

confidence: 84%

Stimulating Capacity of Human Lymphoid Cell Subpopulations in Mixed Lymphocyte Cultures

Albrechtsen¹,

Lied²

1978

Scand J Immunol

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The content and yield or B cells, T cells and monocytes were investigated in cell fractions separated from human peripheral blood by various techniques. These cell fractions, which were highly enriched for either B cells, T cells or monocytes, were tested for their capacity to stimulate allogenic peripheral blood mononuclear cells in mixed lymphocyte cultures (MLC). B cells were found to be strongly stimulatory. Monocytes stimulated strongly at low stimulator/responder cell ratios, while less stimulation was observed using larger amounts of cells. T cells were demonstrated to stimulate weakly, if at all.

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“…With regard to stimula tory potential there is no doubt that B cells are strong stimulators [Han and Dadey, 1976;Potter and Moore, 1976], but there are contradictory reports concerning the T cells. For example, Lehrmann et al [1974b] and found that they pos sessed no stimulatory capacity, while Van Oers et al [1977] in man and Lonai and McDevitt [1977] in mice have demonstrated a consistent stimulator capacity.…”

Section: Discussionmentioning

confidence: 99%

Two Small Lymphocyte Subpopulations in Human Peripheral Blood

Hokland¹,

Hokland²,

Heron³

1978

Int Arch Allergy Immunol

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Two small lymphocyte subpopulations from human peripheral blood were produced by rosette sedimentation procedures, and tested for in vitro functions by stimulation with mitogens, PPD and allogeneic lymphocytes, and for effector capacities in ADCC. The non-T, non-Fc subpopulation had previously been found to be a Smlg⁺ fraction which did not aquire new surface markers during unstimulated culture, did not respond to mitogens, PPD or allogeneic cells but were potent MLC stimulators. In addition, they were poor ADCC effector cells. The non-T, non-B (‘Null’) cells, which showed varying surface marker profiles from donor to donor, were able to respond to mitogens and PPD, but were weak MLC responders. Potent ADCC effector capacity was, however, found to be exhibited by this subpopulation.

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The mixed lymphocyte reaction (MLR) stimulatory capacity of human lymphocyte subpopulations

Cited by 14 publications

References 28 publications

Poor Mixed Lymphocyte Reaction Stimulatory Capacity of Leukemic B Cells of Chronic Lymphocytic Leukemia Patients Despite the Presence of Ia Antigens

Poor Mixed Lymphocyte Reaction Stimulatory Capacity of Leukemic B Cells of Chronic Lymphocytic Leukemia Patients Despite the Presence of Ia Antigens

Stimulating Capacity of Human Lymphoid Cell Subpopulations in Mixed Lymphocyte Cultures

Two Small Lymphocyte Subpopulations in Human Peripheral Blood

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