The mixture of liquid foam soap, ethanol and citric acid as a new fixative–preservative solution in veterinary anatomy

Turan, Erkut; Güleş, Özay; Kilimci, Figen Sevil; Kara, Mehmet Erkut; Dilek, Ömer Gürkan; Sabanci, S. S.; Tatar, Musa

doi:10.1016/j.aanat.2016.09.002

Cited by 15 publications

(26 citation statements)

References 17 publications

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“…Other authors have reported the use of ethanol fixation in human 8,9 and animal cadavers. 19 In our experience, the ethanol-glycerin-benzalkonium chloride fixation is very suitable for the dissection work of students, which is in agreement with Hammer et al 7 and Turan et al 19 Color preservation is convenient for dissection, because it helps the students to the better identify the different structures. Qualitatively, we perceive a more reddish color in the muscles and viscera when starting the dissection.…”

Section: Discussionsupporting

confidence: 86%

“…However, future research needs to uncover why the exposure to air affects so much the color and how to preserve it better. Turan et al 19 measured the color of soft-preserved cadavers with a spectrophotometer and found that the tissues took on a whitish hue, with a posterior change to a greenish hue, but in the present case, the change of color was attributed to the use of citric acid and liquid foam soap.…”

Section: Discussionmentioning

confidence: 55%

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Preservation of Animal Cadavers with a Formaldehyde-free Solution for Gross Anatomy

Tamayo-Arango

Garzón-Alzate

2018

J Morphol Sci

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Motivated by the current health safety regulations at Universidad de Antioquia, our laboratory changed the animal cadavers preserving solution based on formaldehyde, methanol, glycerin and phenol to a formula based on 85% ethanol, 10% glycerin, and 5% benzalkonium chloride. A total of 33 donated cadavers were preserved with this formula so far: 4 goats, 16 dogs, 3 cats and 10 bovine fetuses. Red and blue latex dyes were injected into the vascular systems. Small cadavers were first injected with latex, followed by muscular and intracavitary injection with the preservation fluid and immersion in 96% ethanol. Large cadavers were vascularly injected, wrapped in plastic bags and vascularly repleted with latex during the next 8 days. Samples were taken for microbiological analysis from 3 cadavers: 1 cadaver wrapped with plastic for 2 months, 1 cadaver immersed for 4 months, and 1 cadaver after 15 days of perfusion. The first way to preserve cadavers was more time-consuming, but it rendered cadavers with a more thorough distribution of latex on small arteries and veins. An enhanced flexibility of joints and tissues promoted an easier dissection process, even of the most distal regions, allowing the movement of tendons along their sheaths. Also, a better color preservation was observed in spite of a darkening after the tissues were exposed to the air. There was no gross evidence of decay from bacterial or fungal growth, and the cultures were negative. The most important advantage of this formula is its lower toxicity and cost.

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Section: Discussionsupporting

confidence: 86%

Section: Discussionmentioning

confidence: 55%

Preservation of Animal Cadavers with a Formaldehyde-free Solution for Gross Anatomy

Tamayo-Arango

Garzón-Alzate

2018

J Morphol Sci

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“…Long-term preservation and use are also very important [18]. Moreover, researchers aimed to fi nd specimens harmless for the human health during the lectures [6,14]. There are important contributions to the usage of plastinated specimens as educational material and some studies were stated on these various positive effects on students [3,16].…”

Section: Discussionmentioning

confidence: 99%

“…Color measurements of the semitendinosus muscle, semimembranosus muscle, patellar ligament, and femur were performed using a CR 400/410 Colorimeter device (Konica, Minolta, Korea). These measurements were achieved at the International Commission on Illumination (CIE) standards the L* a* and b* values, where the L* is the brightness of the product on a 0-100 scale; a* indicates the reddish color changes from green to red and b* corresponds to the bluish color change from blue to yellow [13,14]. Colorimetric parameters were measured at 4 different stages; formalin fi xed (Group I) or fresh (Group II), after dehydration, after forced impregnation and at the end of gas curing-hardening stages.…”

Section: Methodsmentioning

confidence: 99%

Differentiation of Anatomic Entities in the Dog Stifle Joint Following S10B Plastination: Comparative Colorimetric and Radiological Investigations

et al. 2019

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The knee joint is not only the most important and complex structure to present during anatomy lectures, but also a significant region for radiologists and orthopedicians. The objectives of this paper are: 1) to evaluate the efficacy of plastinated specimens by measuring colorimetric differentiation and 2) to compare the anatomical structures of the images obtained from computed tomography (CT) and silicone plastination cross-sections and perform three-dimensional (3D) reconstructed models of the specimens. A total of 16 knee joints selected from the 10% formalin fixed/dissected group (n=6), non-fixed/dissected group (n=6) and non-dissected whole knee joint group (n=4) were scanned by CT and plastinated by using the S10B silicone technique. The color changes of the structures were quantitatively determined with the colorimeter device. Plastinated whole knee joints were cut transversally with a band saw and compared with CT images. Finally, 3D reconstruction of the knee joints was performed from CT images with the 3D reconstruction program. There were statistically significant differences between the fixed and non-fixed groups in terms of color changes (p<0.001). The anatomical structures were identified and matched in the cross-sections of plastinates and corresponding CT images. It was observed that plastinated samples were elastic, durable and non-hazardous specimens to use in the veterinary orthopedics and anatomy courses and lectures. It has been found that fixation was useful for the final product morphology. It is thought that the 3D images obtained from this study will support comprehension of the relationships between the bones, muscles, and ligaments.

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“…Formaldehyde causes coagulation, makes discoloration of the tissues different tones of grey rapidly (Brenner, 2014;Janczyk et al, 2011;Natekar and Desouza, 2012;Turan et al, 2017). Because of these problems, fixation solution was not used in the study.…”

Section: Resultsmentioning

confidence: 99%

Evaluation of the acceptability of fresh dog cadavers in anatomy education

Oto

Bakici

Insal

et al. 2018

IJAR

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The usage of cadavers as training material for students and lecturers is limited due to disadvantages of the fixation solutions and methods. These disadvantages include the toxicity of chemicals that are used in fixative solutions in terms of human health, the alteration of properties in fresh tissues and ethical concerns of the fixation processes of cadavers. After physiological death, the alterations eventuate in the cell and tissues due to decomposition from during the first instant of the post-mortem period. The exposure time of fresh cadavers are limited. In this study, it was aimed to determine the color changes of a fresh cadaver after death quantitatively and indicate the usability period of the cadaver under study as educational. material. Besides, it is planned to reduce the use of both live animal casualties and chemical containing fixation solutions. It has been revealed that a fresh cadaver can be used as a training material for a limited period as 7 days. It was also determined that it is more appropriate to use cadaver in education after regional dissection.

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The mixture of liquid foam soap, ethanol and citric acid as a new fixative–preservative solution in veterinary anatomy

Cited by 15 publications

References 17 publications

Preservation of Animal Cadavers with a Formaldehyde-free Solution for Gross Anatomy

Preservation of Animal Cadavers with a Formaldehyde-free Solution for Gross Anatomy

Differentiation of Anatomic Entities in the Dog Stifle Joint Following S10B Plastination: Comparative Colorimetric and Radiological Investigations

Evaluation of the acceptability of fresh dog cadavers in anatomy education

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