The mode of dexamethasone decoration influences avidin-nucleic-acid-nano-assembly organ biodistribution and in vivo drug persistence

Ongaro, Alberto; Violatto, Martina Bruna; Casarin, Elisabetta; Pellerani, Isabella; Marchini, Gloria; Ribaudo, Giovanni; Salmona, Mario; Carbone, Marco; Passoni, Alice; Gnodi, Elisa; Schiavon, Elisa; Mattarei, Andrea; Barisani, Donatella; Invernizzi, Pietro; Bigini, Paolo; Morpurgo, Margherita

doi:10.1016/j.nano.2021.102497

Cited by 4 publications

(21 citation statements)

References 25 publications

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Section: Innovative Approaches To Target Macrophagesmentioning

confidence: 99%

“…ANANAS formulation is based on the Avidin-Nucleic-Acid-Nano-ASsemblies platform and consists in poly-avidin nanoparticles that form upon the high affinity driven nucleation of avidin units around a non-coding plasmid DNA [ 63 ]. ANANAS is made of soft biodegradable and biocompatible components and is poorly immunogenic, since the avidin in the assembly does not induce abnormal immune response in healthy NFR mice [ 63 ]. In the same mouse model, ANANAS formulation possesses some features that make it suitable as a drug carrier to treat a broad range of liver disorders, as many nanoparticle (NP)-based carriers accumulate at the liver due to the filter functions of this organ and independently of the presence of liver targeting moieties [ 63 ].…”

Section: Innovative Approaches To Target Macrophagesmentioning

confidence: 99%

“…ANANAS is made of soft biodegradable and biocompatible components and is poorly immunogenic, since the avidin in the assembly does not induce abnormal immune response in healthy NFR mice [ 63 ]. In the same mouse model, ANANAS formulation possesses some features that make it suitable as a drug carrier to treat a broad range of liver disorders, as many nanoparticle (NP)-based carriers accumulate at the liver due to the filter functions of this organ and independently of the presence of liver targeting moieties [ 63 ]. Furthermore, ANANAS nanoparticles possess a high stability in the bloodstream and a pH dependent release into the target.…”

Section: Innovative Approaches To Target Macrophagesmentioning

confidence: 99%

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The Role of Macrophages in Liver Fibrosis: New Therapeutic Opportunities

Binatti

Gerussi

Barisani

et al. 2022

IJMS

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Chronic inflammation is the hallmark of fibrotic disorders and is characterized by the activation of immune cells in the damaged tissues. Macrophages have emerged as central players in the fibrotic process since they initiate, sustain and amplify the inflammatory reaction. As regards the liver, distinct populations of phagocytic cells, like Kupffer cells and monocyte-derived macrophages, are indisputably key cells implicated in the pathogenesis of several chronic liver diseases. In this review, we summarize the current knowledge on the origin, role and functions of macrophages in fibrotic conditions, with a specific focus on liver fibrosis; then, we discuss some innovative therapeutic strategies targeting macrophages in fibrotic liver diseases.

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Section: Innovative Approaches To Target Macrophagesmentioning

confidence: 99%

Section: Innovative Approaches To Target Macrophagesmentioning

confidence: 99%

Section: Innovative Approaches To Target Macrophagesmentioning

confidence: 99%

See 1 more Smart Citation

The Role of Macrophages in Liver Fibrosis: New Therapeutic Opportunities

Binatti

Gerussi

Barisani

et al. 2022

IJMS

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“…One possible solution is to concentrate the pharmacological activity in the liver, improve the utilization efficiency of drugs and reduce the use of drugs, and thus there is a need to identify solutions that can concentrate drugs in the liver and lower the level of untargeted drugs. 118,119 Avidin-nucleic-acid-nano-assemblies (ANANAS) are a type of poly anti-biotin NPs based on the high affinity-driven nucleation of avidin units around non-coding plasmid DNA. ANANAS are biodegradable and highly tolerated and show a high degree of liver affinity with no need for liver targeting elements.…”

Section: Reviewmentioning

confidence: 99%

Section: Nano-drug Delivery Platform For Autoimmune Hepatitismentioning

confidence: 99%

Recent advances on drug delivery nanoplatforms for the treatment of autoimmune inflammatory diseases

et al. 2022

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Development of a Nanoparticle-Based Approach for the Blood–Brain Barrier Passage in a Murine Model of Amyotrophic Lateral Sclerosis

Violatto

Pasetto

Casarin³

et al. 2022

Cells

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The development of nanoparticles (NPs) to enable the passage of drugs across blood–brain barrier (BBB) represents one of the main challenges in neuropharmacology. In recent years, NPs that are able to transport drugs and interact with brain endothelial cells have been tested. Here, we investigated whether the functionalization of avidin-nucleic-acid-nanoassembly (ANANAS) with apolipoprotein E (ApoE) would allow BBB passage in the SOD1G93A mouse model of amyotrophic lateral sclerosis. Our results demonstrated that ANANAS was able to transiently cross BBB to reach the central nervous system (CNS), and ApoE did not enhance this property. Next, we investigated if ANANAS could improve CNS drug delivery. To this aim, the steroid dexamethasone was covalently linked to ANANAS through an acid-reversible hydrazone bond. Our data showed that the steroid levels in CNS tissues of SOD1G93A mice treated with nanoformulation were below the detection limit. This result demonstrates that the passage of BBB is not sufficient to guarantee the release of the cargo in CNS and that a different strategy for drug tethering should be devised. The present study furthermore highlights that NPs can be useful in improving the passage through biological barriers but may limit the interaction of the therapeutic compound with the specific target.

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The mode of dexamethasone decoration influences avidin-nucleic-acid-nano-assembly organ biodistribution and in vivo drug persistence

Cited by 4 publications

References 25 publications

The Role of Macrophages in Liver Fibrosis: New Therapeutic Opportunities

The Role of Macrophages in Liver Fibrosis: New Therapeutic Opportunities

Recent advances on drug delivery nanoplatforms for the treatment of autoimmune inflammatory diseases

Development of a Nanoparticle-Based Approach for the Blood–Brain Barrier Passage in a Murine Model of Amyotrophic Lateral Sclerosis

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