The Modifying Role of Toxic Substances on Genotoxic Effect in the Body During Combined Administration With Carcinogen (Benzo[a]pyrene)

Abstract

The aim: of this work was to experimentally study the modifying role of toxic substances (phenol) in the manifestation of genotoxic and immunological changes in the body when exposed to a carcinogen (benzo[a]pyrene). Materials and methods: Investigations were carried out in the chronic experiment on white random-bred male mice. Genotoxic (micronucleus test), immunologic and pathomorphological methods were used. Results: As a result of the experiment on white outbred mice during the isolated peroral administration of benzo[a]pyrene (a single dose of 0.1 mg) and in combination with phenol (single doses of 0.1 mg; 0.002 mg) a carcinogenic effect (forestomach papillomas) has been established as well as general patterns of the manifestation of genotoxic and immunological changes regarding carcinogenesis and their dependence on the dose and duration of the administration of the substances in the early stages of the experiment. The established patterns involved parallelism of development and unidirectionality of the genotoxic effect (increasing of micronucleus incidence) and suppression of the T-cell immunity by the end of the month as well as reliable negative correlation between them. Conclusions: It has been established that phenol has a modifying effect on carcinogenesis, which was shown as an increase in the micronuclei frequency, intensification of immunosuppression in the early stages and an increase in the multiplicity factor of the development of forestomach tumors.