The molecular basis of partitioning in aqueous two-phase systems

Huddleston, Jonathan G.; Veide, Andres; Köhler, Kristina; Flanagan, Julia; Enfors, Sven‐Olof; Lyddiatt, Andrew

doi:10.1016/0167-7799(91)90130-a

Cited by 165 publications

(93 citation statements)

References 21 publications

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“…One ATPS theory is based on thermodynamics and states that two factors determine the free energy of mixing of two substances. One is the entropy gain and the other is the interaction between molecules [13]. The gain in entropy is related to the number of molecules involved in mixing and the larger volume accessible to one polymer after mixing.…”

Section: Discussionmentioning

confidence: 99%

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Extraction of IgY from egg yolk using a novel aqueous two-phase system and comparison with other extraction methods

Stålberg

Larsson

2001

Upsala Journal of Medical Sciences

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Egg yolk is an important source of antibodies. The biggest obstacle for isolation of chicken antibodies (IgY) is the removal of lipids, which are present in abundance in egg yolk. We have used a two-phase system to separate egg yolk. The use of an aqueous two-phase system with phosphate and Triton X-100 made separation of lipids and water-soluble proteins possible. Lipids are extracted into the detergentenriched top-phase, whereas IgY is isolated in the phosphate-enriched bottom-phase. The phosphate:triton system was characterised and optimised using various experimental designs. For the optimised model, the yield of IgY was kept above 97% (11.1-14.9 mg IgY/g egg yolk recovered). The amount of lipids in the bottom-phase was kept below 25% of the total content in the egg yolk added. Hence, the model described provides a method for extracting the IgY-fraction with a high yield and relatively low lipid content.

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Section: Discussionmentioning

confidence: 99%

“…In solution, a highly ordered layer of hydration surrounds the polymers. Two chemically distinct polymers will give rise to two different hydration layers [13]. If the water structures are incompatible they will give rise to phase separation.…”

Section: Discussionmentioning

confidence: 99%

Extraction of IgY from egg yolk using a novel aqueous two-phase system and comparison with other extraction methods

Stålberg

Larsson

2001

Upsala Journal of Medical Sciences

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“…This is a simple and rapid method; on the other hand, it is based mainly on visual observation of the transition from the single-phase system to two-phase system. 16,21,29,30,35 . The coexistence curve can shift in the presence of proteins, enzymes and other biomolecules.…”

Section: Aqueous Two-phase Polymeric Systems -Atppsmentioning

confidence: 99%

“…Some inorganic salts are more able to promote this effect (e.g., CaCl 2 , MgCl 2 , AlCl 3 ) when the ions form association complexes with the ether groups. 7 The effects of polymer molar mass, concentration, phase density and the presence of salts in aqueous two-phase polymeric systems have been studied by many authors: Albertsson 6,[18][19][20][26][27][28][29][30][31]41 The partitioning coefficient of proteins and biomolecules in conventional aqueous two-phase polymeric systems is not extreme. In order to enhance partitioning it is possible to add to the system affinity ligands.…”

Section: Aqueous Two-phase Polymeric Systems -Atppsmentioning

confidence: 99%

Liquid–liquid extraction of biomolecules: an overview and update of the main techniques

Mazzola

Lopes

Hasmann

et al. 2007

J of Chemical Tech & Biotech

225

119

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Two-phase systems can be exploited in separation science for the extraction/purification of desired biomolecules. This article reviews recent experimental work on the use of aqueous two-phase polymer systems, two-phase aqueous micellar systems and reversed micellar systems for the extraction/purification of biomolecules. The experimental partitioning behavior of different biomolecules is reviewed and new results with nisin and lipopolysaccharides are presented. An extensive description of each system is presented, based on the biomolecules behavior and the tools employed to improve their extraction and purification.

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“…[24] The performance of salts in promoting phase separation is reflected in the lyotropic series (a classification of ions based upon salting-out or salting-in ability). [25] Salts with multi-charged anions are most effective, e.g. SO 4 2¡ > HPO 4 2¡ > acetate > Cl ¡ , whereas cations are commonly ranked in the following order:…”

Section: Selection Of Phase-forming Saltmentioning

confidence: 99%

Extraction of lipase fromRhizopus microsporusfermentation culture by aqueous two-phase partitioning

Anvari

2015

Biotechnology & Biotechnological Equipment

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The purification of a desired protein from the complex mixture of biomolecules in a fermentation broth is one of the main challenges in industrial-scale biotechnological processes. For partial purification of industrial enzymes, one possible approach is to use aqueous two-phase extraction (APTE) as a primary downstream processing step. In the present study, the feasibility of utilizing ATPE for the purification of lipase from Rhizopus microsporus fermentation culture was investigated. The effects of the phase composition and salt type, molecular weight of the polyethylene glycol (PEG), pH of the system, sample loading and addition of neutral salts on lipase partitioning were investigated at 24 C. In most of the examined aqueous two-phase systems (ATPSs), lipase showed affinity to the top phase. Optimum conditions for lipase purification were obtained in 20% PEG 2000/12% (NH 4 ) 2 SO 4 , with 5% Na 2 CO 3 addition at pH D 8 for 30% crude load. For the optimized ATPS, the recovery yield and purification factor in the top phase were determined to be 92.3% and 19.8, respectively.

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The molecular basis of partitioning in aqueous two-phase systems

Cited by 165 publications

References 21 publications

Extraction of IgY from egg yolk using a novel aqueous two-phase system and comparison with other extraction methods

Extraction of IgY from egg yolk using a novel aqueous two-phase system and comparison with other extraction methods

Liquid–liquid extraction of biomolecules: an overview and update of the main techniques

Extraction of lipase fromRhizopus microsporusfermentation culture by aqueous two-phase partitioning

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