The Molecular Biology of Ilarviruses

Pallás, Vicente; Aparicio, Frederic; Herranz, M. C.; Sánchez‐Navarro, J. A.; Scott, S. W.

doi:10.1016/b978-0-12-407698-3.00005-3

Cited by 96 publications

(105 citation statements)

References 174 publications

Supporting

Mentioning

104

Contrasting

Order By: Relevance

“…PDV and PNRSV belong to the genus Ilarvirus (Bromoviridae) which are composed of a tripartite genome and isometric to bacilliform particles (Fauquet et al, 2005;Pallás et al, 2012Pallás et al, , 2013. PDV-infected trees often remain symptomless but necrotic and chlorotic spots on cherry leaves have also been reported (Massart et al, 2008).…”

Section: Introductionmentioning

confidence: 99%

Main viruses in sweet cherry plantations of Central-Western Spain

Sánchez

Morales-Corts

Benavides

et al. 2015

Sci. agric. (Piracicaba, Braz.)

View full text Add to dashboard Cite

Section: Introductionmentioning

confidence: 99%

Main viruses in sweet cherry plantations of Central-Western Spain

Sánchez

Morales-Corts

Benavides

et al. 2015

Sci. agric. (Piracicaba, Braz.)

View full text Add to dashboard Cite

“…Localization of CP-PDV in ER indicates the site of this protein synthesis and may suggest its potential involvement in the transmission of viral RNA used in the translation. This seems to be probable, because PDV coat protein, like that of other Ilarviruses, has the domain capable of RNA binding (Pallas and Garcia 2011;Pallas et al 2012;Pallas et al 2013). In the case of AMV, there are no reports concerning CP location in the ER, which seems to indicate the difference between this virus and PDV.…”

Section: Immunofluorescent and Immunogold Labeling Localization Of Cpmentioning

confidence: 99%

“…PDV replicase (P1 protein) is probably involved in viral RNA anchoring during replication (Pallas et al 2013). In case of all Bromoviridae, this protein co-localizes with RNA-dependent polymerase RNA (P2 protein) in the postulated areas of replication (Bol 2005;Pallas et al 2012).…”

Section: Immunofluorescent and Immunogold Labeling Localization Of Pdmentioning

confidence: 99%

“…In case of all Bromoviridae, this protein co-localizes with RNA-dependent polymerase RNA (P2 protein) in the postulated areas of replication (Bol 2005;Pallas et al 2012). The process of PDV RNA synthesis requires the formation of a replication complex composed of P1 and P2 proteins, within the appropriate cell organelles (Bol 2005;Pallas et al 2013 Ep-epidermis, PMe-palisade parenchyma, SMe-spongy parenchyma, Pa-parenchyma, X-tracheal element, Ph-phloem, Ne-necrosis chloroplasts, ER, moreover, also in vacuoles, and tonoplast within parenchyma cells. Bamunusinghe et al (2011) pointed out that within Bromoviridae family, the coat protein is near the sites of occurrence of P1 and P2 protein (replication complex).…”

Section: Immunofluorescent and Immunogold Labeling Localization Of Pdmentioning

confidence: 99%

See 1 more Smart Citation

Subcelullar localization of proteins associated with Prune dwarf virus replication

et al. 2017

View full text Add to dashboard Cite

Prune dwarf virus (PDV) is one of the most dangerous pathogens of fruit trees worldwide. One of the most important proteins required for PDV infection is replicase. (P1 protein) which anchored viral RNA and builds replication complex along with RNA depended polymerase. Despite the importance of PDV as a pathogen, our knowledge regarding tissue/cellular localization and structure of PDV P1 protein is still incomplete. The aim of this work was to localize replicase distribution in leaf tissues and cells by immunofluorescent and immunogold labeling of Nicotiana tobaccum cv Samsun and development of a 3D model of PDV replicase. In this paper we demonstrate that PDV replication, is similar to that of Alfalfa mosaic virus and is strongly connected with tonoplasts. In addition, PDV replicase and coat protein (CP) were also found to be strongly associated with membranes of endoplasmic reticulum and, indicating the potential involvement of these membrane structures in the processes related to viral infection. Bioinformatic analyzes based on 3D modeling and structure prediction revealed that P1 protein has a potential transmembrane domain which enables protein anchoring to tonoplast during replication complex assembly.

show abstract

“…One arginine located in the N thermal part of CP is fundamental in RNA-binding activity (Yusibov and Loesch-Fries, 1998). In the case of PDV, the crucial arginine is located at position 14 of the RNAbinding consensus sequence (Q/K/R-P/N-T-X-RS-R/Q-Q/N/S-W/F-A) (Pallas et al, 2013).…”

mentioning

confidence: 99%

Genetic variability among coat protein of Prune dwarf virus variants from different countries and different Prunus species

et al. 2014

View full text Add to dashboard Cite

Prune dwarf virus (PDV) isolates have been investigated for genetic diversity. Full-length nucleotide and amino acid sequences of viral coat protein from 23 isolates collected from different stone fruit trees (sour and sweet cherry trees, wild cherry tree, plum tree, almond tree, peach tree) and different countries (Poland, Italy, Germany, USA, Israel) were analysed and compared to 57 others available in GenBank. Comparison of all sequenced virus isolates revealed diversity of 86-100 % at nucleotide level and 79-100 % at amino acid level. The ratio of non-synonymous to synonymous polymorphic sites indicated that purifying selection dominated in case of PDV. However, six codons showed to be under strong positive selection, including the codon located inside the structure involved in RNAbinding activity.

show abstract

The Molecular Biology of Ilarviruses

Cited by 96 publications

References 174 publications

Main viruses in sweet cherry plantations of Central-Western Spain

Main viruses in sweet cherry plantations of Central-Western Spain

Subcelullar localization of proteins associated with Prune dwarf virus replication

Genetic variability among coat protein of Prune dwarf virus variants from different countries and different Prunus species

Contact Info

Product

Resources

About