2010
DOI: 10.2478/s11658-010-0029-x
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The molecular cloning of glial fibrillary acidic protein in Gekko japonicus and its expression changes after spinal cord transection

Abstract: The glial fibrillary acidic protein (GFAP) is an astrocyte-specific member of the class III intermediate filament proteins. It is generally used as a specific marker of astrocytes in the central nervous system (CNS). We isolated a GFAP cDNA from the brain and spinal cord cDNA library of Gekko japonicus, and prepared polyclonal antibodies against gecko GFAP to provide useful tools for further immunochemistry studies. Both the real-time quantitative PCR and western blot results revealed that the expression of GF… Show more

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Cited by 9 publications
(11 citation statements)
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References 49 publications
(47 reference statements)
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“…japonicus GFAP expression was higher at 1 week after SCI and decreased 4 weeks after the operation ( Fig 1 ). The results are consistent with the previous reports about the GFAP expression pattern after spinal cord transection [ 21 , 22 ], and indicate that astroglial activation was attenuated in adult G . japonicus compared to in adult rats.…”
Section: Resultssupporting
confidence: 93%
“…japonicus GFAP expression was higher at 1 week after SCI and decreased 4 weeks after the operation ( Fig 1 ). The results are consistent with the previous reports about the GFAP expression pattern after spinal cord transection [ 21 , 22 ], and indicate that astroglial activation was attenuated in adult G . japonicus compared to in adult rats.…”
Section: Resultssupporting
confidence: 93%
“…We conducted cooling anesthesia to minimize their suffering. Geckos received complete spinal cord transections at the L10–11 lumbar vertebrae level using procedures in our lab described previously [16]. Briefly, after laminectomy, the spinal cord was transected using iridectomy scissors.…”
Section: Methodsmentioning
confidence: 99%
“…For the purpose of investigating the central nervous system of geckos, especially addressing diverse changes of injured neural cells after gecko CNS trauma, some species-specific neural cells marker genes need to be obtained. To date, the complete open read frame (ORF) information of the GFAP [16], TUBB3 [17] gene and their corresponding polyclonal antibodies have been obtained in our laboratory. In the present study, we aimed to clone and characterize the enolase2 gene.…”
Section: Introductionmentioning
confidence: 99%
“…It has been generally regarded that the “real” stellate astrocytes are absent from anamniotes (Lyons and Talbot, 2014), in spite of some unrepeatable adverse reports (Kawai et al, 2001; Alunni et al, 2005). Works in Gekko japonicus , a reptile, have revealed that the animal can regenerate spinal cord following injury, without evoking astrocytic responses (Gao et al, 2010; Gu et al, 2015). The molecular cues with respect to promoting the neuronal survival and mediating the events of non-reactive astrocytes, have not been fully clarified in the regenerative animals.…”
Section: Introductionmentioning
confidence: 99%