The molecular machinery for secretion is conserved from yeast to neurons.

Abstract

A variety of approaches have been utilized to identify and characterize the molecules that mediate vesicular trafficking along the secretory pathway. Two approaches that have been particularly fruitful include the genetic dissection of the yeast secretory pathway and the biochemical characterization of proteins involved in the synaptic vesicle membrane trafficking in the mammalian nerve terminal. The recent convergence of these approaches suggests that common mechanisms may underlie a wide variety of vesicle-mediated transport steps. We discuss the results that support this possibility and propose a model for synaptic vesicle docking and fusion that incorporates evolutionarily conserved elements that may be part of a constitutive fusion machinery and specialized elements that may mediate regulatory events that are specific to the process of neurotransmitter release.

Apolar growth of Neurospora crassa leads to increased secretion of extracellular proteins

“…Unfortunately, little is known about the molecular mechanisms underlying protein secretion in filamentous fungi. The protein secretion pathway has been studied most extensively in Saccharomyces cerevisiae and mammalian cells and has been found to be very similar (Bennett and Scheller, 1993). Therefore, we may expect many of the features of the protein secretion pathway observed in S. cerevisiae and mammalian cells to be conserved in filamentous fungi.…”

Chromogranin A: current status as a precursor for bioactive peptides and a granulogenic/sorting factor in the regulated secretory pathway

“…Is there a CGA analog, for example, in yeast suggesting a 'preendocrine' secretory role akin to the roles played by proteins of the 'sec' family in extracellular secretion in yeast compared to endocrine and neuronal secretion in mammals [137].…”

“…Chromostatin is a fragment of chromogranin A (CGA- (124)(125)(126)(127)(128)(129)(130)(131)(132)(133)(134)(135)(136)(137)(138)(139)(140)(141)(142)(143) in the cow) which represents a special case of potential extracellular processing of CGA. Simon and co-workers described an inhibitory effect of purified chromogranin A on catecholamine release from the adrenal medulla.…”

A Rab1 mutant affecting guanine nucleotide exchange promotes disassembly of the Golgi apparatus.

“…However, we have been unable to obtain any further evidence relevant to this point from analysis of rabla mutants defective in phosphorylation (Davidson, H. W., and W. E. Balch, unpublished observations). Any of the other transport components involved in vesicle targeting or fusion (9,49) would also be expected to be excellent targets for phosphorylation control.…”