The Molecular Neighborhood of Subunit 8 of Yeast Mitochondrial F1F0-ATP Synthase Probed by Cysteine Scanning Mutagenesis and Chemical Modification

Stephens, Andrew N.; Khan, Muhammad Altaf; Roucou, Xavier; Nagley, Phillip; Devenish, Rodney J.

doi:10.1074/jbc.m300967200

Cited by 38 publications

(38 citation statements)

References 63 publications

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“…This residue, which is non-conserved, was susceptible to cross-linking from the intermembrane space with three components of the peripheral stalk (subunits 4, 8, and i) that are located in its neighborhood (29,43,44). In this work, we found that a cross-linking product involving subunit 6 and showing a relative mass of 47,000 was obtained upon incubation of wild-type mitochondria with CuCl 2 (Fig.…”

Section: Resultsmentioning

confidence: 52%

“…Subunit 6 is located in the periphery of the ATP synthase in contact with the membranous rotor with which it forms the proton channel. It belongs to the peripheral stalk of the ATP synthase and likely interacts with other components of the stalk such as subunits 4 (b), i, and 8, as shown by cross-linking experiments (29,43,44). In this work, we produced a disulfide bridge between two subunits 6 via their Cys 23 upon oxidation of either mitochondria or the solubilized supramolecular forms of ATP synthase.…”

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confidence: 99%

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Evidence of the Proximity of ATP Synthase Subunits 6 (a) in the Inner Mitochondrial Membrane and in the Supramolecular Forms of Saccharomyces cerevisiae ATP Synthase

Velours

Stinès-Chaumeil

Habersetzer

et al. 2011

Journal of Biological Chemistry

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Background:The mitochondrial ATP synthase adopts oligomeric structures. Results: Cross-linking between two subunits 6 (a) indicates their proximity in the ATP synthase dimer. Conclusion: Subunit 6 participates in the ATP synthase monomer-monomer interface. Significance: Learning how the ATP synthases assemble is essential for understanding their involvement in the control of the biogenesis of the inner mitochondrial membrane.

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Section: Resultsmentioning

confidence: 52%

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confidence: 99%

Evidence of the Proximity of ATP Synthase Subunits 6 (a) in the Inner Mitochondrial Membrane and in the Supramolecular Forms of Saccharomyces cerevisiae ATP Synthase

Velours

Stinès-Chaumeil

Habersetzer

et al. 2011

Journal of Biological Chemistry

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“…ATP6 and ATP8 are the two mtDNA-encoded subunits that are incorporate into Complex V where they play essential roles in the final assembly of ATPase [52,62]. Studies in yeast demonstrated that the N-terminal signal-peptide domain of ATP8 is responsible for its function [63], whereas the C-terminal domain is required for the assembly of F 0 [64] and the transmembrane stem accommodates charged AA residues [65]. The Artemia ATP8 proteins shares a common four-residue sequence motif (IPQM) at their N-termini, which is similar to the ATP8-specific motif (MPQM) identified previously in other animals [66].…”

Section: Protein-coding Genesmentioning

confidence: 99%

Mitochondrial genome sequences of Artemia tibetiana and Artemia urmiana: assessing molecular changes for high plateau adaptation

Zhang

Luo

Sun

et al. 2013

Sci. China Life Sci.

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Brine shrimps, Artemia (Crustacea, Anostraca), inhabit hypersaline environments and have a broad geographical distribution from sea level to high plateaus. Artemia therefore possess significant genetic diversity, which gives them their outstanding adaptability. To understand this remarkable plasticity, we sequenced the mitochondrial genomes of two Artemia tibetiana isolates from the Tibetan Plateau in China and one Artemia urmiana isolate from Lake Urmia in Iran and compared them with the genome of a low-altitude Artemia, A. franciscana. We compared the ratio of the rate of nonsynonymous (Ka) and synonymous (Ks) substitutions (Ka/Ks ratio) in the mitochondrial protein-coding gene sequences and found that atp8 had the highest Ka/Ks ratios in comparisons of A. franciscana with either A. tibetiana or A. urmiana and that atp6 had the highest Ka/Ks ratio between A. tibetiana and A. urmiana. Atp6 may have experienced strong selective pressure for high-altitude adaptation because although A. tibetiana and A. urmiana are closely related they live at different altitudes. We identified two extended termination-associated sequences and three conserved sequence blocks in the D-loop region of the mitochondrial genomes. We propose that sequence variations in the D-loop region and in the subunits of the respiratory chain complexes independently or collectively contribute to the adaptation of Artemia to different altitudes. mitochondrial genome, Artemia tibetiana, sequence variation, high plateau species Citation:Zhang H X, Luo Q B, Sun J, et al. Mitochondrial genome sequences of Artemia tibetiana and Artemia urmiana: assessing molecular changes for high plateau adaptation. Sci China Life Sci, 2013Sci, , 56: 440 -452, doi: 10.1007 Brine shrimps, Artemia (Crustacea, Anostraca), have a broad geographical distribution and inhabit hypersaline environments that vary considerably in their anionic composition, climate, and altitude. The major anions that contribute to anionic composition include chloride, sulfate, carbonate, or combinations of up to all three [1]. Artemia species can be found in altitudes from sea level to over 4000 m above sea level; for example, in Tibet (4000 m) and in the East African Plateau (over 5000 m). Artemia can survive in harsh environments other than in high salinity, including low atmospheric pressure, rarefied air, cold temperature, and long winters, as well as in climatological conditions that range from humid to arid [2]. Artemia species are capable of switching their reproduction strategies between sexual and parthenogenetic forms in a single life cycle [1,3,4], and populations can survive through cold winters by producing diapause cysts [5][6][7]. Because of their distinct features, Ar- Zhang H X, et al. Sci China Life Sci May (2013) Vol.56 No.5 441 temia draw significant scientific interest from several areas including ecology, aquaculture, physiology, ecotoxicology, and genetics [8][9][10][11][12]. Animal mitochondrial (mt) genomes are circular DNA molecules ~17 kb in length that encode the ma...

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“…The addition of Atp6p at a late stage of assembly may prevent the exchange of protons across the membrane through the partially assembled F 0 sector. This scheme is still very fragmentary and is lacking a host of intermolecular interactions of other F 0 subunits needed to stabilize the final complex (1,2,13).…”

mentioning

confidence: 99%

Atp10p Assists Assembly of Atp6p into the F0 Unit of the Yeast Mitochondrial ATPase

Tzagoloff

Barrientos

Neupert

et al. 2004

Journal of Biological Chemistry

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The F 0 F 1 -ATPase complex of yeast mitochondria contains three mitochondrial and at least 17 nuclear gene products. The coordinate assembly of mitochondrial and cytosolic translation products relies on chaperones and specific factors that stabilize the pools of some unassembled subunits. Atp10p was identified as a mitochondrial inner membrane component necessary for the biogenesis of the hydrophobic F 0 sector of the ATPase. Here we show that, following its synthesis on mitochondrial ribosomes, subunit 6 of the ATPase (Atp6p) can be cross-linked to Atp10p. This interaction is required for the integration of Atp6p into a partially assembled subcomplex of the ATPase. Pulse labeling and chase of mitochondrial translation products in vivo indicate that Atp6p is less stable and more rapidly degraded in an atp10 null mutant than in wild type. Based on these observations, we propose Atp10p to be an Atp6p-specific chaperone that facilitates the incorporation of Atp6p into an intermediate subcomplex of ATPase subunits.

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The Molecular Neighborhood of Subunit 8 of Yeast Mitochondrial F1F0-ATP Synthase Probed by Cysteine Scanning Mutagenesis and Chemical Modification

Cited by 38 publications

References 63 publications

Evidence of the Proximity of ATP Synthase Subunits 6 (a) in the Inner Mitochondrial Membrane and in the Supramolecular Forms of Saccharomyces cerevisiae ATP Synthase

Evidence of the Proximity of ATP Synthase Subunits 6 (a) in the Inner Mitochondrial Membrane and in the Supramolecular Forms of Saccharomyces cerevisiae ATP Synthase

Mitochondrial genome sequences of Artemia tibetiana and Artemia urmiana: assessing molecular changes for high plateau adaptation

Atp10p Assists Assembly of Atp6p into the F0 Unit of the Yeast Mitochondrial ATPase

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