The Molecular Virology of Enteric Viruses

Buesa, Javier; Rodrı́guez-Dı́az, Jesús

doi:10.1007/978-3-319-30723-7_3

Cited by 12 publications

(16 citation statements)

References 433 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In the present study the capsid region that defines the norovirus genotype was targeted [9]. Most strains resulted to be of genogroup II, reflecting the higher circulation of the genogroup in the population [15].…”

Section: Discussionmentioning

confidence: 90%

“…Despite this, the methodology applied allowed us to identify several noroviruses GI and GII. It is known that noroviruses GII have a wider circulation than noroviruses GI, playing a major role in acute gastroenteritis [15]. In the present study the capsid region that defines the norovirus genotype was targeted [9].…”

Section: Discussionmentioning

confidence: 95%

“…So far, at least 36 G-genotypes and 51 P-genotypes have been identified among human and animal rotaviruses [14]. Viruses carrying G1P [8], G2P [4], G3P [8], and G4P [8] represent over 90% of human rotavirus strains co-circulating in most countries, although other G and P combinations like G9P [8] and G12P [8] are being isolated in increasing numbers [15].…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Epidemiological Surveillance of Norovirus and Rotavirus in Sewage (2016–2017) in Valencia (Spain)

Santiso-Bellón,

Randazzo,

Pérez-Cataluña

et al. 2020

Microorganisms

Self Cite

View full text Add to dashboard Cite

The aim of the present study was to perform the molecular epidemiology of rotaviruses and noroviruses detected in sewage samples from a large wastewater facility from the city of Valencia, Spain. A total of 46 sewage samples were collected over a one-year period (September 2016 to September 2017). Norovirus and rotavirus were detected and quantified by RT-qPCR, genotyped by semi-nested RT-PCR and further characterized by sequencing and phylogenetic analyses. Noroviruses and rotaviruses were widely distributed in sewage samples (69.6% for norovirus GI, 76.0% norovirus GII, and 71.7% rotaviruses) and viral loads varied from 4.33 to 5.75 log PCRU/L for norovirus GI, 4.69 to 6.95 log PCRU/L for norovirus GII, and 4.08 to 6.92 log PCRU/L for rotavirus. Overall, 87.5% (28/32) of GI noroviruses could not be genotyped, 6.25% (2/32) of the samples contained GI.2 genotype, and another 6.25% (2/32) were positive for GI.4 genotype. The most common genotype of GII noroviruses was GII.2 (40%, 14/35), followed by GII.6 (8.6%, 3/35) and GII.17 (5.7%, 2/35) while the remaining GII strains could not be typed (45.7%, 16/35). Rotavirus VP4 genotype P[8] was the only one found in 19 out of 33 rotavirus-positive samples (57.7%). G2 was the most prevalent rotavirus VP7 genotype (15.2%, 5/33) followed by G3, G9, and G12, with two positive samples for each genotype (6.1%, 2/33). In one sample both G1 and G2 genotypes were detected simultaneously (3%). The results presented here show that the surveillance of noroviruses and rotaviruses in sewage is useful for the study of their transmission in the population and their molecular epidemiology.

show abstract

Section: Discussionmentioning

confidence: 90%

Section: Discussionmentioning

confidence: 95%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Epidemiological Surveillance of Norovirus and Rotavirus in Sewage (2016–2017) in Valencia (Spain)

Santiso-Bellón,

Randazzo,

Pérez-Cataluña

et al. 2020

Microorganisms

Self Cite

View full text Add to dashboard Cite

show abstract

Section: Importancementioning

confidence: 99%

“…N oroviruses (NoVs) are the predominant etiological agents of acute gastroenteritis worldwide, causing both outbreaks and sporadic cases (1-3). In many countries, NoVs have become the main cause of infantile gastroenteritis since the introduction of rotavirus vaccines (4-7), and they have also been recognized globally as the main cause of associated foodborne diseases (8,9).NoVs belong to the family Caliciviridae, and currently, they are classified into 6 genogroups (GI to GVI) (10) subdivided into more than 30 genotypes based on the capsid protein sequence diversity. Nevertheless, most human NoV infections are caused by genogroups GI and GII.…”

mentioning

confidence: 99%

Characterization of a Novel Conformational GII.4 Norovirus Epitope: Implications for Norovirus-Host Interactions

Carmona-Vicente

Vila-Vicent

Allen

et al. 2016

J Virol

Self Cite

View full text Add to dashboard Cite

Human noroviruses (NoVs) are the main etiological agents of acute gastroenteritis worldwide. While NoVs are highly diverse (more than 30 genotypes have been detected in humans), during the last 40 years most outbreaks and epidemics have been caused by GII.4 genotype strains, raising questions about their persistence in the population. Among other potential explanations, immune evasion is considered to be a main driver of their success. In order to study antibody recognition and evasion in detail, we analyzed a conformational epitope recognized by a monoclonal antibody (3C3G3) by phage display, site-directed mutagenesis, and surface plasmon resonance. Our results show that the predicted epitope is composed of 11 amino acids within the P domain: P245, E247, I389, Q390, R397, R435, G443, Y444, P445, N446, and D448. Only two of them, R397 and D448, differ from the homologous variant (GII.4 Den-Haag_2006b) and from a previous variant (GII.4 VA387_1996) that is not recognized by the antibody. A double mutant derived from the VA387_1996 variant containing both changes, Q396R and N447D, is recognized by the 3C3G3 monoclonal antibody, confirming the participation of the two sites in the epitope recognized by the antibody. Furthermore, a single change, Q396R, is able to modify the histo-blood group antigen (HBGA) recognition pattern. These results provide evidence that the epitope recognized by the 3C3G3 antibody is involved in the virus-host interactions, both at the immunological and at the receptor levels. IMPORTANCEHuman noroviruses are the main cause of viral diarrhea worldwide in people of all ages. Noroviruses can infect individuals who had been previously exposed to the same or different norovirus genotypes. Norovirus genotype GII.4 has been reported to be most prevalent during the last 40 years. In the present study, we describe a novel viral epitope identified by a monoclonal antibody and located within the highly diverse P domain of the capsid protein. The evolution of this epitope along with sequential GII.4 variants has allowed noroviruses to evade previously elicited antibodies, thus explaining how the GII.4 genotype can persist over long periods, reinfecting the population. Our results also show that the epitope participates in the recognition of host receptors that have evolved over time, as well. N oroviruses (NoVs) are the predominant etiological agents of acute gastroenteritis worldwide, causing both outbreaks and sporadic cases (1-3). In many countries, NoVs have become the main cause of infantile gastroenteritis since the introduction of rotavirus vaccines (4-7), and they have also been recognized globally as the main cause of associated foodborne diseases (8,9).NoVs belong to the family Caliciviridae, and currently, they are classified into 6 genogroups (GI to GVI) (10) subdivided into more than 30 genotypes based on the capsid protein sequence diversity. Nevertheless, most human NoV infections are caused by genogroups GI and GII. Furthermore, in the last 2 decades, genotype GII.4 has been th...

show abstract

A Comprehensive Review on Human Aichi Virus

Rivadulla

Romalde

2020

Virol. Sin.

View full text Add to dashboard Cite

Although norovirus, rotavirus, adenovirus and Astrovirus are considered the most important viral agents transmitted by food and water, in recent years other viruses, such as Aichi virus (AiV), have emerged as responsible for gastroenteritis outbreaks associated with different foods. AiV belongs to the genus Kobuvirus of the family Picornaviridae. It is a virus with icosahedral morphology that presents a single stranded RNA genome with positive sense (8280 nucleotides) and a poly (A) chain. AiV was first detected from clinical samples and in recent years has been involved in acute gastroenteritis outbreaks from different world regions. Furthermore, several studies conducted in Japan, Germany, France, Tunisia and Spain showed a high prevalence of AiV antibodies in adults (between 80% and 99%), which is indicative of a large exposure to this virus. The aim of this review is to bring together all the discovered information about the emerging pathogen human Aichi virus (AiV), discussing the possibles routes of transmission, new detection techniques and future research. Although AiV is responsible for a low percentage of gastroenteritis outbreaks, the high seroprevalence shown by human populations indicates an evident role as an enteric agent. The low percentage of AiV detection could be explained by the fact that the pathogen is more associated to subclinical infections. Further studies will be needed to clarify the real impact of AiV in human health and its importance as a causative gastroenteritis agent worldwide.

show abstract

The Molecular Virology of Enteric Viruses

Cited by 12 publications

References 433 publications

Epidemiological Surveillance of Norovirus and Rotavirus in Sewage (2016–2017) in Valencia (Spain)

Epidemiological Surveillance of Norovirus and Rotavirus in Sewage (2016–2017) in Valencia (Spain)

Characterization of a Novel Conformational GII.4 Norovirus Epitope: Implications for Norovirus-Host Interactions

A Comprehensive Review on Human Aichi Virus

Contact Info

Product

Resources

About