The monoclonal antibody CZ‐1 identifies a mouse CD45‐associated epitope expressed on interleukin‐2‐responsive cells

Brutkiewicz, Randy R.; O’Donnell, Carey L.; Maciaszek, Joseph Walter; Welsh, Raymond M.; Vargas‐Cortes, Mauricio

doi:10.1002/eji.1830231008

Cited by 4 publications

(5 citation statements)

References 31 publications

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“…To determine if this sensitivity to inactivation may have been due to differences in sialic acid content between the human and mouse cell lines, SV passaged through these lines was treated 1:1 with 0.3 U of neuraminidase per ml before exposure to NHS. Although 0.5 ml of this same neuraminidase solution removed a sialic acid-dependent CD45 epitope from splenocytes (detectable by MAb CZ-1 [7]) (data not shown), the treatment of SV in the present experiment had no significant effect on the pattern of viral inactivation ( Table 4, experiment 5).…”

Section: Patterns Of Inactivation Of Other Enveloped Virusesmentioning

confidence: 57%

Evaluation of the Galα1-3Gal Epitope as a Host Modification Factor Eliciting Natural Humoral Immunity to Enveloped Viruses

Welsh

O’Donnell

Reed

et al. 1998

J Virol

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Human sera contain high levels of natural antibody (Ab) to Gal␣1-3Gal, a terminal glycosidic structure expressed on the surface of cells of mammals other than Old World primates. Incorporation of this determinant onto retroviral membranes by passage of viruses in cells encoding ␣-1-3-galactosyltransferase (GT) renders retroviruses sensitive to lysis by natural Ab and complement in normal human serum (NHS). Plasma membrane-budding viruses representing four additional virus groups were examined for their sensitivities to serum inactivation after passage through human cell lines that lack a functional GT or human cells expressing recombinant porcine GT. The inactivation of lymphocytic choriomeningitis virus (LCMV) by NHS directly correlated with host modification of the virus via expression of Gal␣1-3Gal and was blocked by incorporation of soluble Gal␣1-3Gal disaccharide into the inactivation assay. GT-deficient mice immunized to make high levels of Ab to Gal␣1-3Gal (anti-Gal Ab) were tested for resistance to LCMV passaged in GT-expressing cells. Resistance was not observed, but in vitro analyses of the mouse immune sera revealed that the antiviral activity of the sera was insufficient to eliminate LCMV infectivity on its natural targets of infection, macrophages, which express receptors for Ab and complement. Newcastle disease virus and vesicular stomatitis virus (VSV) were inactivated by NHS regardless of cell passage history, whereas Sindbis virus (SV) passaged in human cells resisted inactivation. Both VSV and SV passaged in Gal␣1-3Gal-expressing human cells incorporated thissugar moiety onto their major envelope glycoproteins. SV passaged in mouse cells expressing Gal␣1-3Gal was moderately sensitive to inactivation by NHS. These results indicate that enveloped viruses expressing Gal␣1-3Gal differ in their sensitivities to NHS and that a potent complement source, such as that in NHS, is required for efficient inactivation of sensitive viruses in vitro and in vivo.

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Section: Patterns Of Inactivation Of Other Enveloped Virusesmentioning

confidence: 57%

Evaluation of the Galα1-3Gal Epitope as a Host Modification Factor Eliciting Natural Humoral Immunity to Enveloped Viruses

Welsh

O’Donnell

Reed

et al. 1998

J Virol

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“…Previously, the authors have demonstrated by both complement-mediated depletion and cell sorting, that all mature splenic NK cells express CZ-1 [37]. Furthermore, CZ-1 is expressed on all splenic cells that proliferate in response to IL-2 [31]. It has been shown previously that the bone marrow contains non-lytic immature NK lineage cells (3A4* NKI.I ) which, when cultured in the presence of low amounts of IL-2, can differentiate into functional mature NK cells [30.…”

Section: Expression Of Cz-1 On Ll-2-responsive Nk Cell Precursors In mentioning

confidence: 99%

“…2 B). During the course of these studies, it became apparent that the CZ-I antibody recognized a sialic acid-dependent epitope of CD45RB [31], Therefore, the expression of CD45RB on Ly6* Lin c-kit* cells was examined. An identical staining pattern of CD45RB expression on Ly6*^ Lin" c-kit*^ cells was seen when a commercially available anti-CD45RB antibody (clone 16A) was used in place of CZ-1 (data not shown).…”

Section: Fig 5 Expression Of Cz-i On Ly6+mentioning

confidence: 99%

“…Splenic NK cells, CD8* T cells, and B cells express this epitope. Also, all splenic cells capable of responding to IL-2 are found within the CZ-I ' population [31]. Interestingly, not all splenic CD45RB'^ cells (detected using a commercial anti-CD45RB Ab) stain with the CZ-I antibody, but all CZ-1+ cells coexpress CD45RB.…”

Section: Introductionmentioning

confidence: 99%

“…This paper describes the expression of CZ-I on pro-and pre-N K cells. The CZ-1 antibody has been shown to recognize a sialic acid-dependent epitope of CD45RB [31]. Splenic NK cells, CD8* T cells, and B cells express this epitope.…”

Section: Introductionmentioning

confidence: 99%

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Expression of CZ‐1: a CD45RB Epitope on Progenitors of Natural Killer and Other Haematopoietic Cells

et al. 1994

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CZ-1 is a novel sialic acid-dependent epitope of the murine CD45RB molecule which is expressed on cells that proliferate when cultured in IL-2. Because IL-2 appears to be important in the differentiation of NK cells, the authors examined the expression of CZ-1 on immature NK-lineage cells within the bone marrow. All mature NK1.1+ cells as well as their NK1.1- IL-2 responsive precursors were CZ-1+. Furthermore, IL-2 unresponsive transplantable NK progenitor cells expressed CZ-1 also. To examine expression of CZ-1 on other immature lymphoid progenitor cells, CZ-1+ and CZ-1- marrow cells were transplanted into lightly irradiated scid mice. Transfer of CZ-1+ cells resulted in rapid and sustained generation of thymocytes and splenic B cells, whereas CZ-1- cells caused delayed repopulation. This suggested that the slowly repopulating pluripotent stem cells lacked CZ-1. Therefore, expression of CZ-1 on Ly6+ Lin- c-kit+ cells, highly enriched for pluripotent stem cells, was examined. This population appeared to be homogeneously CZ-1dull. Thus, it appears that expression of CZ-1 is developmentally regulated, with differentiation associated with increased expression. Since CZ-1 is expressed on a protein tyrosine phosphatase, it is likely that this molecule regulates differentiation of NK and other lymphoid cells.

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T-cell activation results in microheterogeneous changes in glycosylation of CD45

Hernandez

Klein²,

Dyken

et al. 2007

International Immunology

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During T-cell development and activation, dramatic changes occur in glycan structures that decorate cell-surface glycoproteins. These changes have been considered to be general cellular events that affect many glycans on many glycoproteins. For example, loss of sialic acid from core 1 O-glycans on T-cell surface glycoproteins CD45, CD43 and CD8, detected with peanut agglutinin (PNA), is a hallmark of immature thymocytes and activated peripheral T cells. Loss of cell-surface sialic acid during T-cell activation has been proposed to enhance TCR reactivity with antigen. However, CD4 T-cell activation also results in increased binding of the CZ-1 antibody that recognizes a sialic acid-containing epitope on CD45RB. This indicates that increased sialylation of the CZ-1 epitope occurs during CD4 T cell activation, and that loss of cell surface sialic acid during T-cell activation is a selective event rather than affecting all cell surface glycans. As specific glycans on specific glycoprotein backbones control critical events in T-cell maturation and survival, understanding mechanisms of selective glycoprotein glycosylation is important for regulating T-cell development and function. We define the sialylated O-glycan epitope recognized by CZ-1, and find that, paradoxically, CZ-1 and PNA binding are simultaneously increased on activated CD4(+) T cells, demonstrating site-specific changes in CD45 sialylation. Moreover, we identify ST3Gal I as the sialyltransferase responsible for creating the CZ-1 epitope. Thus, changes in glycan structure during T-cell activation are microheterogeneous and unique to individual glycans on specific glycoproteins, implying that these glycans have precise functions in T-cell biology.

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The monoclonal antibody CZ‐1 identifies a mouse CD45‐associated epitope expressed on interleukin‐2‐responsive cells

Cited by 4 publications

References 31 publications

Evaluation of the Galα1-3Gal Epitope as a Host Modification Factor Eliciting Natural Humoral Immunity to Enveloped Viruses

Evaluation of the Galα1-3Gal Epitope as a Host Modification Factor Eliciting Natural Humoral Immunity to Enveloped Viruses

Expression of CZ‐1: a CD45RB Epitope on Progenitors of Natural Killer and Other Haematopoietic Cells

T-cell activation results in microheterogeneous changes in glycosylation of CD45

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