The Morphological, Anatomical, And Physiological Characteristics of Elephantopus scaber As Explant Source For Tissue Culture

Yuliani, Yuliani; Dewi, Sari Kusuma; Rachmadiarti, Fida

doi:10.2991/icst-18.2018.13

Cited by 2 publications

(3 citation statements)

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“…Propagation of E. scaber using leaf explants had been carried out [9], but there were still few studies which used seed explants from E. scaber for callus induction. Callus induction from E. scaber leaves as explants is not optimal, due to trichomes in E. scaber leaves inhibit tissue initiation to become callus, mostly cause of microbial contamination [10]. Thus, seed explants were used in this study to induce E. scaber callus.…”

Section: Resultsmentioning

confidence: 99%

In-vitro Propagation of Elephantopus scaber Using Seeds as Explants in Various Culture Growth Media

Yuliani¹,

Rachmadiarti²,

Dewi³

et al. 2019

Proceedings of the Mathematics, Informatics, Science, and Education International Conference (MISEIC 2019)

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Elephantopus scaber plant contains various secondary metabolites, such as flavonoids, phenolics, saponins, terpenes, triterpenoids, sesquiterpenes lactones, elephantopyns, and deoxyelephantopin, which can act as antimicrobial, antifungal, and bioinsecticides. Elephantopus scaber can be potentially grown by tissue culture method and isolated its secondary compounds. But, the presence of tricomes at stem, leaf midrib, or leaves of E. scaber hinder its in-vitro propagation, because of it can facilitate contamination agents, such as fungi and bacteria. To avoid contamination, seeds were used as source of explants. The aim of this study was to describe the use of seeds as an alternative explant source of E. scaber in various culture growth medium and to determine the right medium for E. scaber callus induction. This research used four growth mediums: 1) MSO medium (with no additional Plant Growth Factor), 2) MSC medium (MS medium with additional charcoal), 3) MS + 0.5 mg/L BAP and 1 mg/L 2.4 D, and 4) MS + 1.5 mg/L Kinetin and 1.5 mg/L 2.4 D. There were 20 bottles media for experiment with five seed explants put into each bottle of medium, thus total of 100 seeds were planted. The propagation steps were sterilization of mature seeds, preparation and sterilization of growth medium, and inoculation of seeds. The observation of callus induction was performed for four weeks. The parameters observed in the research was the formation of the callus induced from seeds of E. scaber. The results showed that explants in 5 bottles had grown, 7 seeds were planted as seedlings, while seeds in 15 bottles showed no signs of growth. All seeds planted in MSC medium (20 bottles) produced seedlings and explants. About 82 calli from 20 bottles were induced on MS + 0.5 mg/L BAP and 1 mg/L 2.4 D, while 17 bottles of MS + 1.5 mg/L Kinetin and 1.5 mg/L 2.4 D produced 48 calli and 3 bottles produced seedlings. The results showed that seed explants of E. scaber could reduce microbe contamination because there was no tricomes in seeds, as opposite on the leaves during callus induction. Seeds of E. scaber could be effectively used as an explant for callus induction through in-vitro propagation.The most optimal medium for callus induction of E. scaber seed was MS + 0.5 mg/L BAP and 1 mg/L 2.4 D. These findings support recommendation for callus induction using the seeds for plants with numerous tricomes on their leaves, such as Elephantopus scaber.

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Section: Resultsmentioning

confidence: 99%

In-vitro Propagation of Elephantopus scaber Using Seeds as Explants in Various Culture Growth Media

Yuliani¹,

Rachmadiarti²,

Dewi³

et al. 2019

Proceedings of the Mathematics, Informatics, Science, and Education International Conference (MISEIC 2019)

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“…Active compounds likely cause decreases in the total number of microbes with antimicrobial effects. The secondary metabolites identified in E. scaber leaves include flavonoids, saponins, terpenoids, and phenols, which can all act as antimicrobials and can inhibit microbial growth, which may eventually lead to microbial death (HUSSAIN & SALEEM 2009, YULIANI et al 2018. KUMAR et al (2004) stated that E. scaber leaf extracts can act as an antibacterial for various bacteria, including Pseudomonas aeruginosa, Proteus vulgaris, Bacillus subtilis, and Escherichia coli.…”

Section: Discussionmentioning

confidence: 99%

“…Elephantopus scaber Linn. contains several secondary metabolites, such as flavonoids (methanol leaf extracts), alkaloids, tannins (methanol and chloroform leaf extracts), phenols (methanol leaf-rhizome extracts), proteins, glycosides, saponins (methanol rhizome extracts), terpenoids (methanol and chloroform leaf-rhizome extracts), steroids (methanol and chloroform leaf-rhizome extracts), triterpenoid, and elephantopin (YULIANI et al 2018). E. scaber extracts also contain epifriedelinol, lupeol, stigmasterol, lupeol acetate, deoxyelephantopin, isodeoxyelephantopin, sesquiterpene, and luteolin-7-glucoside, which demonstrate various pharmacological activities, such as anti-tumor, anti-cancer, anti-inflammatory, antibacterial, and antifungal activities (FARHA & REMANI 2014).…”

Section: Introductionmentioning

confidence: 99%

Bioactivity effect of Elephantopus scaber Linn. extracts against Spodoptera litura and the soil microbial community

Yuliani

2022

Rev. Ciênc. Agrovet.

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This study investigates the biopesticidal effects of Elephantopus scaber Linn. extract on mortality of Spodoptera litura, Plutella xylostella, and non-target organisms and investigate the impact on S. litura protein levels and soil microbial community structure. The experiment was performed using a completely randomized design. Methanol extracts from E. scaber leaves, at concentrations of 2%, 4%, 6%, 8%, 10%, and 12%, were tested for bioactivity against the 2nd-instar larva of S. litura, P. xylostella, and earthworms. Mortality rates of the larvae and worms were observed. The collected data were analyzed using analysis of variance (ANOVA), followed by probit and descriptive analysis. The results showed that methanol extracts of E. scaber (12%) influenced the highest mortality rates for both S. litura (93.35%) and P. xylostella (96.65%) with LC50 and LC80 of S. litura was 1.867 and 4.763; for P. xylostella were 4.488 and 7.92, respectively. However, the application of E. scaber biopesticide also influences earthworms’ mortality rate. The 6% E. scaber extract resulted in 60% death of earthworms during a 20-days period. In addition, higher concentrations of E. scaber extracts resulted in lower molecular weights and levels of S. litura proteins. The diversity and density of the soil microbial community also decreased by 6% concentration.

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The Morphological, Anatomical, And Physiological Characteristics of Elephantopus scaber As Explant Source For Tissue Culture

Cited by 2 publications

References 6 publications

In-vitro Propagation of Elephantopus scaber Using Seeds as Explants in Various Culture Growth Media

In-vitro Propagation of Elephantopus scaber Using Seeds as Explants in Various Culture Growth Media

Bioactivity effect of Elephantopus scaber Linn. extracts against Spodoptera litura and the soil microbial community

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