The morphology of human cells from the choroid and retinal pigment epithelium grown in vitro on isolated Bruch's membrane

Abstract

Cells from samples of human choroidal tissue were collected and seeded onto isolated Bruch's membrane in vitro, and the morphology of the developing cultures was examined by light-, scanning electron- and transmission electron-microscopy. In early cultures, the cells showed a flattened, spread out appearance with short surface villi and a low degree of cellular overlapping. After 48 h, a shift towards a more elongated cell form was observed, and one week old cultures were composed of predominantly elongated cells with a pronounced degree of overlapping. In contrast, human retinal pigment epithelial cells seeded onto sheets of Bruch's membrane maintained a spread out epithelial morphology with a varying degree of overlapping throughout the culture period. The present system permits comparative studies on the behavior of choroidal and pigment epithelial cells under controlled conditions. It may serve as an in vitro model for disorders characterized by growth of these cells on Bruch's membrane in vivo.

In vitro ingrowth of choroidal cells on Bruch's membrane

“…It has previously been shown that human choriocapillaris and choroidal cells seeded on Bruch's membrane can be studied in controlled tissue culture conditions (Overskott Vatne 1988;Nicolaissen et al 1988).…”