The motility and fertility of golden hamster sperm cultured in BSA‐free medium

Uto, Norihiko; Yamahama, Yumi

doi:10.1016/s0248-4900(97)86827-x

Cited by 12 publications

(7 citation statements)

References 14 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…BSA supplementation (1 g/100 mL) in the semen extender also increased the sperm motion characteristics and viability. These results are consistent with results of Lornage et al (1983) and Uto & Yamahama (1996) who found higher percentages of motile and viable spermatozoa in the groups with BSA supplementation. The axosome and associated dense fibers of the middle pieces in sperm are covered by mitochondria that generate energy from intracellular stores of ATP.…”

Section: Discussionsupporting

confidence: 93%

Antioxidant effects of bovine serum albumin on kinetics, microscopic and oxidative characters of cryopreserved bull spermatozoa

Ashrafi

Kohram

Tayefi‐Nasrabadi

2013

Span. j. agric. res.

View full text Add to dashboard Cite

The aim of this study was to determine the effects of bovine serum albumin (BSA) as an antioxidant on post-thaw characters of bull spermatozoa such as motion variables, viability, plasma membrane integrity, morphology, lipid peroxidation, total antioxidant capacity (TAC), total thiols (TT) and the enzymes activities. Ejaculates were collected from six proven bulls and diluted with a citrate-based extender supplemented with various concentrations of BSA (0, 0.5, 1, 1.5 and 2 g/100 mL). The results showed that the semen extender supplemented with various concentrations of BSA increased (p0.05) were detected in progressive motility, sperm track straightness, beat cross frequency, curvilinear velocity and malondialdehyde production between the experimental groups. The highest concentration of BSA (2 g/100 mL) in the semen extender reduced (p<0.05) the sperm motion variables. The results indicated that the most effective concentration of BSA is 1 g/100 mL in the semen extender which is associated with an increase in the total motility, antioxidant enzymes activities, TT and TAC.

show abstract

Section: Discussionsupporting

confidence: 93%

Antioxidant effects of bovine serum albumin on kinetics, microscopic and oxidative characters of cryopreserved bull spermatozoa

Ashrafi

Kohram

Tayefi‐Nasrabadi

2013

Span. j. agric. res.

View full text Add to dashboard Cite

show abstract

“…With respect to the motility canine sperm in the medium containing PVA or BSA showed a high motility. This spermatozoon is not different from other species, where the albumin or PVA is essential to maintain sperm motility and vitality (Harrison et al 1978, 1982; Bavister 1981; Uto and Yamahama 1996).…”

Section: Discussionmentioning

confidence: 75%

Effect of Albumin and Polyvinyl Alcohol on the Vitality, Motility and Acrosomal Integrity of Canine Spermatozoa Incubated in vitro

Risopatrón

Catalán

Miska

et al. 2002

Reprod Domestic Animals

View full text Add to dashboard Cite

Sperm culture media used for in vitro fertilization (IVF) procedures are important factors concerning the viability, motility and acrosomal integrity of spermatozoa. The aim of this study was to investigate the effects of three different sperm diluting media, tissue culture medium (TCM-199), sperm culture medium (Sp-TALP) and human tubular fluid (HTF) supplemented with varying concentrations of bovine serum albumin (1, 4 and 6%) or polyvinyl alcohol (0.8%) on the acrosomal integrity, motility and viability of canine spermatozoa. Ejaculates collected from four dogs were diluted in all media and spermatozoa were separated from seminal plasma by the swim-up technique. Sperm progressive motility was assessed using a phase contrast microscope. Viability and acrosomal integrity were evaluated using a dual stain technique (Giemsa-Trypan blue). The results demonstrated that the number of live canine spermatozoa was similar in culture media supplemented or not supplemented with macromolecules. A minimal concentration of albumin (1%) in the three media showed similar effects on vitality, motility and acrosomal integrity, as had higher concentrations (4 and 6%). The percentage of acrosome-intact spermatozoa was markedly higher after HTF (94.1%) than after TCM-199 (70.1%) or Sp-TALP (71.0%) without supplementation. It is concluded that serum bovine albumin, irrespective of the concentration, preserved sperm viability and function, and HTF is the most suitable medium for preserving the acrosome in canine spermatozoa prepared for in vitro manipulation through short incubation.

show abstract

“…This study showed that fatty acids play an important role in improving sperm motility. Motility of rabbit 1 and hamster 13 spermatozoa was improved by fatty acids bound to BSA; boosting of motility might have been the result of supplying sufficient energy from fatty acids bound to BSA. The viability was also improved by fatty acids bound to BSA‐V.…”

Section: Discussionmentioning

confidence: 99%

Effect of fatty acids bound to bovine serum albumin‐V on acrosome reaction and utilization of glucose in boar spermatozoa

Hossain

Hyeong

Miah

et al. 2007

Reprod Medicine & Biology

View full text Add to dashboard Cite

The present study has been designed with the objective of determining if fatty acids bound to bovine serum albumin-V (BSA-V) can improve motility, viability, and increase acrosome reaction (AR) and utilization of glucose in boar spermatozoa. Boar spermatozoa were washed, swum-up and incubated at 37°C for 6 h in TALP medium supplemented with fatty acids bound to bovine serum albumin fraction V (BSA-V), fatty acid free BSA (BSA-FAF), polyvinyl alcohol + main fatty acids bound to BSA-V (PVA + FA) and PVA. Sperm motility, viability, AR, and the incorporation and oxidation ofC-glucose were evaluated during 6 h of incubation. The results show that the BSA-V was superior to BSA-FAF and PVA in improving motility and AR. Viability was significantly increased ( < 0.05) by only BSA-V compared with PVA. When the main fatty acids compound of BSA-V were added to PVA, the sperm motility, viability and AR became almost the same as with BSA-V. The rate of incorporation and oxidation of C-glucose were significantly increased ( < 0.05) by BSA-V compared with BSA-FAF and PVA. Fatty acids bound to BSA-V are important for improvement of sperm functions. The present study postulates that fatty acids bound to BSA-V are important to acrosome reaction and the utilization of glucose in boar spermatozoa.

show abstract

The motility and fertility of golden hamster sperm cultured in BSA‐free medium

Cited by 12 publications

References 14 publications

Antioxidant effects of bovine serum albumin on kinetics, microscopic and oxidative characters of cryopreserved bull spermatozoa

Antioxidant effects of bovine serum albumin on kinetics, microscopic and oxidative characters of cryopreserved bull spermatozoa

Effect of Albumin and Polyvinyl Alcohol on the Vitality, Motility and Acrosomal Integrity of Canine Spermatozoa Incubated in vitro

Effect of fatty acids bound to bovine serum albumin‐V on acrosome reaction and utilization of glucose in boar spermatozoa

Contact Info

Product

Resources

About