The musculature of <i>Squatinella rostrum</i> (Milne, 1886) (Rotifera: Lepadellidae) as revealed by confocal laser scanning microscopy with additional new data on its trophi and overall morphology

Wilts, Eike F.; Wulfken, Diana; Ahlrichs, Wilko H.; Arbizu, Pedro Martínez

doi:10.1111/j.1463-6395.2010.00476.x

Cited by 11 publications

(5 citation statements)

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“…The only muscle having this arrangement is the musculus ramo-manubricus found in Filinia longiseta [ 41 ] and Trichocerca rattus [ 33 ], both having very peculiar trophi (respectively malleoramate and asymmetrical virgate). In Rotifera, though, the posterior connection is well documented in the abundant work of the series of confocal and TEM studies by the Ahlrichs Group [ 31 , 32 , 39 – 41 ], who refers to this muscle as the musculus fulcro ramicus. Furthermore, Riemann and Ahlrichs, emphasize the wide repartition of this muscle within Rotifera, suggesting the homology of this muscle across the taxon [ 39 ].…”

Section: Discussionmentioning

confidence: 99%

“…Recently, several CLSM studies of phalloidin-stained musculature have been carried out on a great number of microscopic animals, revealing comprehensive information on their overall musculature [ 9 , 11 , 28 – 30 ] and also, in the case of gnathiferans, on the musculature of the rotifer mastax [ 31 , 32 ] or gnathostomulid pharynx [ 26 ]. Combined with TEM, many details can be inferred on the relative position of muscles and their ultrastructure, but also connections to the other part of the body.…”

Section: Introductionmentioning

confidence: 99%

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Detailed reconstruction of the musculature in Limnognathia maerski (Micrognathozoa) and comparison with other Gnathifera

et al. 2014

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IntroductionLimnognathia maerski is the single species of the recently described taxon, Micrognathozoa. The most conspicuous character of this animal is the complex set of jaws, which resembles an even more intricate version of the trophi of Rotifera and the jaws of Gnathostomulida. Whereas the jaws of Limnognathia maerski previously have been subject to close examinations, the related musculature and other organ systems are far less studied. Here we provide a detailed study of the body and jaw musculature of Limnognathia maerski, employing confocal laser scanning microscopy of phalloidin stained musculature as well as transmission electron microscopy (TEM).ResultsThis study reveals a complex body wall musculature, comprising six pairs of main longitudinal muscles and 13 pairs of trunk dorso-ventral muscles. Most longitudinal muscles span the length of the body and some fibers even branch off and continue anteriorly into the head and posteriorly into the abdomen, forming a complex musculature. The musculature of the jaw apparatus shows several pairs of striated muscles largely related to the fibularium and the main jaws. The jaw articulation and function of major and minor muscle pairs are discussed. No circular muscles or intestinal musculature have been found, but some newly discovered muscles may supply the anal opening.ConclusionsThe organization in Limnognathia maerski of the longitudinal and dorso-ventral muscle bundles in a loose grid is more similar to the organization found in rotifers rather than gnathostomulids. Although the dorso-ventral musculature is probably not homologous to the circular muscles of rotifers, a similar function in body extension is suggested. Additionally, a functional comparison between the jaw musculature of Limnognathia maerski, Rotifera and Gnathostomulida, emphasizes the important role of the fibularium in Limnognathia maerski, and suggests a closer functional resemblance to the jaw organization in Rotifera.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Detailed reconstruction of the musculature in Limnognathia maerski (Micrognathozoa) and comparison with other Gnathifera

et al. 2014

View full text Add to dashboard Cite

show abstract

“…The only muscle having this arrangement is the musculus ramo-manubricus found in Filinia longiseta [41] and Trichocerca rattus [33], both having very peculiar trophi (respectively malleoramate and asymmetrical virgate). In Rotifera, though, the posterior connection is well documented in the abundant work of the series of confocal and TEM studies by the Ahlrichs Group [31,32,[39][40][41], who refers to this muscle as the musculus fulcro ramicus. Furthermore, Riemann and Ahlrichs, emphasize the wide repartition of this muscle within Rotifera, suggesting the homology of this muscle across the taxon [39].…”

Section: Comparison Of the Pharyngeal Musculature Of L Maerski With mentioning

confidence: 98%

“…Recently, several CLSM studies of phalloidin-stained musculature have been carried out on a great number of microscopic animals, revealing comprehensive information on their overall musculature [9,11,[28][29][30] and also, in the case of gnathiferans, on the musculature of the rotifer mastax [31,32] or gnathostomulid pharynx [26]. Combined with TEM, many details can be inferred on the relative position of muscles and their ultrastructure, but also connections to the other part of the body.…”

Section: Introductionmentioning

confidence: 99%

Detailed reconstruction of the musculature in Limnognathia maerski (Micrognathozoa) and comparison with other Gnathifera

et al. 2014

View full text Add to dashboard Cite

Introduction: Limnognathia maerski is the single species of the recently described taxon, Micrognathozoa. The most conspicuous character of this animal is the complex set of jaws, which resembles an even more intricate version of the trophi of Rotifera and the jaws of Gnathostomulida. Whereas the jaws of Limnognathia maerski previously have been subject to close examinations, the related musculature and other organ systems are far less studied. Here we provide a detailed study of the body and jaw musculature of Limnognathia maerski, employing confocal laser scanning microscopy of phalloidin stained musculature as well as transmission electron microscopy (TEM). Results: This study reveals a complex body wall musculature, comprising six pairs of main longitudinal muscles and 13 pairs of trunk dorso-ventral muscles. Most longitudinal muscles span the length of the body and some fibers even branch off and continue anteriorly into the head and posteriorly into the abdomen, forming a complex musculature. The musculature of the jaw apparatus shows several pairs of striated muscles largely related to the fibularium and the main jaws. The jaw articulation and function of major and minor muscle pairs are discussed. No circular muscles or intestinal musculature have been found, but some newly discovered muscles may supply the anal opening. Conclusions: The organization in Limnognathia maerski of the longitudinal and dorso-ventral muscle bundles in a loose grid is more similar to the organization found in rotifers rather than gnathostomulids. Although the dorso-ventral musculature is probably not homologous to the circular muscles of rotifers, a similar function in body extension is suggested. Additionally, a functional comparison between the jaw musculature of Limnognathia maerski, Rotifera and Gnathostomulida, emphasizes the important role of the fibularium in Limnognathia maerski, and suggests a closer functional resemblance to the jaw organization in Rotifera.

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“…Confocal laser scanning microscopy (CLSM) is a powerful tool that can be used for studying a variety of organisms [1][2][3][4][5][6][7][8][9][10]. Using CLSM is an excellent way to examine the internal and external features of microscopic organisms in detail without compromising the structural integrity of specimens or rendering specimens unusable for further analyses.…”

Section: Introductionmentioning

confidence: 99%

Application of Autofluorescence for Confocal Microscopy to Aid in Archaeoparasitological Analyses

Morrow

Elowsky

2019

Korean J Parasitol

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Confocal laser scanning microscopy (CLSM) was used to examine archaeoparasitological specimens from coprolites associated with La Cueva de los Muertos Chiquitos (CMC) located near present-day Durango, Mexico. The eggs for 4 different types of parasites recovered from CMC coprolites were imaged using CLSM to assist with identification efforts. While some of the parasite eggs recovered from CMC coprolites were readily identified using standard light microscopy (LM), CLSM provided useful data for more challenging identifications by highlighting subtle morphological features and enhancing visualization of parasite egg anatomy. While other advanced microscopy techniques, such as scanning electron microscopy (SEM), may also detect cryptic identifying characters, CLSM is less destructive to the specimens. Utilizing CLSM allows for subsequent examinations, such as molecular analyses, that cannot be performed following SEM sample preparation and imaging. Furthermore, CLSM detects intrinsic autofluorescence molecules, making improved identification independent of resource and time-intensive protocols. These aspects of CLSM make it an excellent method for assisting in taxonomic identification and for acquiring more detailed images of archaeoparasitological specimens.

show abstract

The musculature of Squatinella rostrum (Milne, 1886) (Rotifera: Lepadellidae) as revealed by confocal laser scanning microscopy with additional new data on its trophi and overall morphology

Cited by 11 publications

References 31 publications

Detailed reconstruction of the musculature in Limnognathia maerski (Micrognathozoa) and comparison with other Gnathifera

Detailed reconstruction of the musculature in Limnognathia maerski (Micrognathozoa) and comparison with other Gnathifera

Detailed reconstruction of the musculature in Limnognathia maerski (Micrognathozoa) and comparison with other Gnathifera

Application of Autofluorescence for Confocal Microscopy to Aid in Archaeoparasitological Analyses

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