The mutagenic, DNA-damaging and antioxidative properties of bark and leaf extracts from Coutarea hexandra (Jacq.) K. Schum

Nunes, Líria Granato; Gontijo, Douglas Costa; Souza, Carlos J. A. de; Fietto, Luciano Gomes; Carvalho, André F; Leite, João Paulo Viana

doi:10.1016/j.etap.2011.12.003

Cited by 15 publications

(11 citation statements)

References 28 publications

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“…The total flavonoids were quantified by the aluminum chloride method using rutin as the reference compound and the absorbance measured in a UV-Visible spectrometer after 15 min of reaction at room temperature in the dark [ 41 , 42 ]. An aliquot of 10.0 mg of the ES was suspended by sonication in 1.0 mL of methanol and then 8 mL of methanol, 0.6 mL of acetic acid, and 2.5 mL of 8% aluminum chloride hexahydrate in methanol (w/v) were added.…”

Section: Methodsmentioning

confidence: 99%

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Hydroethanolic Extract of Strychnos pseudoquina Accelerates Skin Wound Healing by Modulating the Oxidative Status and Microstructural Reorganization of Scar Tissue in Experimental Type I Diabetes

Sarandy

Novaes

Xavier

et al. 2017

BioMed Research International

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The effect of topical application of ointment based on Strychnos pseudoquina hydroethanolic extract in the cutaneous wounds healing in diabetic rats was evaluated. Samples of S. pseudoquina were submitted to phytochemical prospection and in vitro antioxidant assay. Thirty Wistar rats were divided into 5 groups: Sal-wounds treated with 0.9% saline solution; VH-wounds treated with 0.6 g of lanolin cream (vehicle); SS-wounds treated with silver sulfadiazine cream (10 mg/g); ES5- and ES10-wounds treated with an ointment of S. pseudoquina extract, 5% and 10%, respectively. Fragments of wounds were removed for histological and biochemical analysis every 7 days during 21 days. ES showed equivalent levels per gram of extract of total phenols and flavonoids equal to 122.04 mg for TAE and 0.60 mg for RE. The chlorogenic acid was one of the major constituents. S. pseudoquina extract presented high antioxidant potential in vitro. ES5 and ES10 showed higher wound healing rate and higher amount of cells, blood vessels, and type III and I collagen. The oxidative stress markers were lower in the ES5 and ES10 groups, while the antioxidants enzymes levels were higher. Ointment based on S. pseudoquina extract promotes a fast and efficient cutaneous repair in diabetic rats.

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Section: Methodsmentioning

confidence: 99%

“…The solvent flow rate was 0.3 ml/min in a column temperature of 23°C. The ionization method used in the mass spectrometry was ESI (Electrospray Ionization) following these conditions: gas temperature of 300°C, nitrogen flow rate of 10 L/min, nebulizer pressure of 35 psi, and capillary voltage of 4000 V [ 41 , 42 ].…”

Section: Methodsmentioning

confidence: 99%

Hydroethanolic Extract of Strychnos pseudoquina Accelerates Skin Wound Healing by Modulating the Oxidative Status and Microstructural Reorganization of Scar Tissue in Experimental Type I Diabetes

Sarandy

Novaes

Xavier

et al. 2017

BioMed Research International

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“…Toxicity and Ames test:T he toxicity evaluation against NIH3T3 fibroblasts and Ames test were performed as previously described following published procedures. [17,38,39] Cytotoxicity on human macrophages was tested with the CytoTox 96 Non-Radioactive Cytotoxicity Assay kit (Promega) according to the manufacturer's instructions.…”

Section: Biological Evaluationmentioning

confidence: 99%

Development of Potent Inhibitors of the Mycobacterium tuberculosis Virulence Factor Zmp1 and Evaluation of Their Effect on Mycobacterial Survival inside Macrophages

et al. 2018

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The enzyme Zmp1 is a zinc-containing peptidase that plays a critical role in the pathogenicity of Mycobacterium tuberculosis. Herein we describe the identification of a small set of Zmp1 inhibitors based on a novel 8-hydroxyquinoline-2-hydroxamate scaffold. Among the synthesized compounds, N-(benzyloxy)-8-hydroxyquinoline-2-carboxamide (1 c) was found to be the most potent Zmp1 inhibitor known to date, and its binding mode was analyzed both by kinetics studies and molecular modeling, identifying critical interactions of 1 c with the zinc ion and residues in the active site. The effect of 1 c on intracellular Mycobacterium survival was assayed in J774 murine macrophages infected with M. tuberculosis H37Rv or M. bovis BCG and human monocyte-derived macrophages infected with M. tuberculosis H37Rv. Cytotoxicity and genotoxicity were also assessed. Overall, inhibitor 1 c displays interesting in vitro antitubercular properties worthy of further investigation.

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“…Despite being considered the main source of antimutagenics and antioxidants ( Çelik and Aslantürk, 2010 ) some of their phytochemicals may cause adverse reactions or have potential of interacting with other medications, generating toxic, cytotoxic, mutagenic and genotoxic effects ( Pawlowski et al , 2012 ; Ping et al , 2012 ; Ray et al , 2013 ). However, for many of them there is little information available regarding the potential health risks at short, mid- or long term ( Nunes et al , 2012 ).…”

Section: Introductionmentioning

confidence: 99%

Genotoxic potential of the latex from cotton-leaf physicnut (Jatropha gossypiifolia L.)

et al. 2015

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Jatropha gossypiifolia L. (Euphorbiaceae), popularly known as cotton-leaf physicnut, is a milky shrub notable for its medicinal properties. The present study aimed to evaluate the toxic, cytotoxic and genotoxic effects of the latex of J. gossypiifolia, using Allium cepa L. as test system. Seeds of A. cepa were exposed to five concentrations of the latex (1.25; 2.5; 5; 10 and 20 mL/L) in order to evaluate parameters of toxicity (evaluation of root growth), cytotoxicity (mitotic index frequency) and genotoxicity (frequency of chromosome alterations). The latex showed a significant decrease in root mean growth value as well as mitotic index for the tested concentrations, except for 1.25 mL/L, when compared to results from the negative control. The 1.25, 2.5 and 5 mL/L concentrations induced significant chromo-some adherences, C-metaphases and/or chromosome bridges, as genotoxic effects. The significant frequency of chromosome bridges also indicated mutagenic potential for chromosomes of J. gossypiifolia as discussed in the paper. Considering that the latex is used in popular therapies, and that the test system A. cepa presents good correlation with tests carried out in mammals, it can be pointed out that its use for medicinal purposes may be harmful to human health especially if ingested.

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The mutagenic, DNA-damaging and antioxidative properties of bark and leaf extracts from Coutarea hexandra (Jacq.) K. Schum

Cited by 15 publications

References 28 publications

Hydroethanolic Extract of Strychnos pseudoquina Accelerates Skin Wound Healing by Modulating the Oxidative Status and Microstructural Reorganization of Scar Tissue in Experimental Type I Diabetes

Hydroethanolic Extract of Strychnos pseudoquina Accelerates Skin Wound Healing by Modulating the Oxidative Status and Microstructural Reorganization of Scar Tissue in Experimental Type I Diabetes

Development of Potent Inhibitors of the Mycobacterium tuberculosis Virulence Factor Zmp1 and Evaluation of Their Effect on Mycobacterial Survival inside Macrophages

Genotoxic potential of the latex from cotton-leaf physicnut (Jatropha gossypiifolia L.)

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