The N‐terminal amino acid sequences of <i>Brevibacterium</i> sp. R312 nitrile hydratase

Duran, Robert; Chion, C.K.N. Chan Kwo; Bigey, Frédéric; Arnaud, A.; Galzy, P.

doi:10.1002/jobm.3620320104

Cited by 13 publications

(6 citation statements)

References 17 publications

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“…The results indicated that the zeta potential value of Co 3 (PO 4 ) 2 nanoflowers was approximately +3.5 mV, and the zeta potential value of NHase@Co 3 (PO 4 ) 2 was −1.7 mV under the neutral conditions. It was known that the isoelectric point of NHase was ∼5.5 and it could be negative under the neutral conditions. , It could be obviously observed that the zeta potential of NHase@Co 3 (PO 4 ) 2 showed a tendency to be negative, because of the addition of NHase. When the matrix and enzyme were oppositely charged, it would have a maximal binding efficiency between the protein and matrix .…”

Section: Resultsmentioning

confidence: 97%

Preparation of a Flowerlike Nanobiocatalyst System via Biomimetic Mineralization of Cobalt Phosphate with Enzyme

Song

Gao

et al. 2017

Ind. Eng. Chem. Res.

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This study reported a novel and facile method for the spontaneous synthesis of flowerlike cobalt phosphate nanocrystals (Co 3 (PO 4 ) 2 nanoflowers) without adding any templates, and the growth mechanism was further studied. Subsequently, an excellent nanobiocatalyst system was established via the biomimetic mineralization of cobalt phosphate with Co-type nitrile hydratase (NHase). Because of the interactions between Co ions in the microenvironment of cobalt phosphate and enzyme active site, the encapsulated NHase (NHase@Co 3 (PO 4 ) 2 ) exhibited high catalytic efficiencies and desirable stabilities. The enzymatic activity of encapsulated NHase was 238.4 U/g and the protein loading amount was 210.73 mg/g. Compared with free NHase, the optimum temperature of NHase@Co 3 (PO 4 ) 2 was 40 °C, which was higher than that of the free NHase (30 °C). The thermal, pH, antiproteolytic, and storage stability of NHase@Co 3 (PO 4 ) 2 were all improved. Furthermore, NHase@Co 3 (PO 4 ) 2 could be applied in the production of nicotinamide (NAM) with a satisfying yield and it could be reused seven times. All these results in this work clearly confirmed that the cobalt phosphate nanocrystals, as an original nanocarrier, would have promising applications for constructing nanobiocatalyst systems.

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Section: Resultsmentioning

confidence: 97%

Preparation of a Flowerlike Nanobiocatalyst System via Biomimetic Mineralization of Cobalt Phosphate with Enzyme

Song

Gao

et al. 2017

Ind. Eng. Chem. Res.

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“…The standard assays for nitrile hydratase activity were carried out as described previously [4]. One unit of nitrile hydratase activity is defined as the amount of enzyme required for hydrolysing 1 μmol of acrylonitrile per min.…”

Section: Methodsmentioning

confidence: 99%

“…R312 (formerly Brevibacterium sp. R312) [4], Rhodococcus erythropolis [5] which contain also pyroloquinoline quinone as prosthetic group and (2) cobalt-containing NHases in Rhodococcus rhodochrous J1 [6], R. rhodochrous M8 [7], Pseudomonas putida [8], Bacillus pallidus Dac521 [9], and Bacillus sp. BR449 [10].…”

Section: Introductionmentioning

confidence: 99%

Rapid and specific identification of nitrile hydratase (NHase)-encoding genes in soil samples by polymerase chain reaction

Precigou

Goulas

Duran

2001

FEMS Microbiology Letters

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A polymerase chain reaction (PCR) protocol was developed for the specific detection of genes coding nitrile hydratase (NHase). Primer design was based on the highly conserved sequences found in the coding region of the alpha-subunit gene corresponding to the metal-binding site. Purified genomic DNA from bacterial strains or directly from soil can serve as the target for the PCR, thus affording a simple and rapid method for screening NHase genes. The primer pairs, NHCo1/NHCo2 and NHFe1/NHFe2 yield PCR products corresponding to a partial coding sequence of cobalt and iron NHase genes, respectively. Using the PCR method, both types of iron- and cobalt-NHase-encoding genes were detected in DNA from pure cultures and soil samples. Furthermore consensus primers allowed rapid cloning and expression of novel NHases in Escherichia coli.

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“…The standard assays for nitrile hydratase activity were carried out as described previously [4]. One unit of nitrile hydratase activity is de¢ned as the amount of enzyme required for hydrolysing 1 Wmol of acrylonitrile per min.…”

Section: Nitrile Hydratase Activitymentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Rapid and specific identification of nitrile hydratase (NHase)-encoding genes in soil samples by polymerase chain reaction

Precigou

2001

FEMS Microbiology Letters

View full text Add to dashboard Cite

A polymerase chain reaction (PCR) protocol was developed for the specific detection of genes coding nitrile hydratase (NHase). Primer design was based on the highly conserved sequences found in the coding region of the K-subunit gene corresponding to the metal-binding site. Purified genomic DNA from bacterial strains or directly from soil can serve as the target for the PCR, thus affording a simple and rapid method for screening NHase genes. The primer pairs, NHCo1/NHCo2 and NHFe1/NHFe2 yield PCR products corresponding to a partial coding sequence of cobalt and iron NHase genes, respectively. Using the PCR method, both types of iron-and cobalt-NHase-encoding genes were detected in DNA from pure cultures and soil samples. Furthermore consensus primers allowed rapid cloning and expression of novel NHases in Escherichia coli. ß

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The N‐terminal amino acid sequences of Brevibacterium sp. R312 nitrile hydratase

Cited by 13 publications

References 17 publications

Preparation of a Flowerlike Nanobiocatalyst System via Biomimetic Mineralization of Cobalt Phosphate with Enzyme

Preparation of a Flowerlike Nanobiocatalyst System via Biomimetic Mineralization of Cobalt Phosphate with Enzyme

Rapid and specific identification of nitrile hydratase (NHase)-encoding genes in soil samples by polymerase chain reaction

Rapid and specific identification of nitrile hydratase (NHase)-encoding genes in soil samples by polymerase chain reaction

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