The natural plant product tryptanthrin ameliorates dextran sodium sulfate-induced colitis in mice

Inhibition of 5-Lipoxygenase Product Synthesis by Natural Compounds of Plant Origin

“…Isaindigotone 100 scavenges superoxide released by PMNL and inhibits cell-free 5-LO with an IC 50 = 0.04 mM. Both alkaloids also inhibited the formation of COX products and NOS-1, and tryptanthrin 99 has proven effective in certain animal models of inflammation [186], [187].…”

Tryptanthrin Inhibits Interferon-.GAMMA. Production by Peyer's Patch Lymphocytes Derived from Mice That Had Been Orally Administered Staphylococcal Enterotoxin.

“…Although SEBactivated PP lymphocytes exhibit not only elevated IFN-g mRNA, but also elevated mRNAs for other pro-inflammatory cytokines such as IL-2 and TNF-a, 22) it is likely that the oral administration of SEB alone in our experiments was not sufficient to induce a large amount of the cytokines. Considering that tryptanthrin inhibited not only IFN-g and IL-2 production in vitro in this study, but also IL-2 production in vivo, 8) it is possible that tryptanthrin inhibits the in vivo production of other inflammatory cytokines, even when these are produced in response to SEB in disease.…”

Modulatory Effects and Action Mechanisms of Tryptanthrin on Murine Myeloid Leukemia Cells

“…Reaction mixture containing 40 units of RN ASE OUT TM recombinant ribonuclease inhibitor, 1 M-MLV first strand buffer, 0.5 mM of each dNTP, 10 mM DTT, 0.1g oligo (dT) [12][13][14][15][16][17][18] and 200 units of M-MLV reverse transcriptase was prepared, and 2 g RNA sample was added into 20 l reaction mixture. A negative control using DEPCtreated water instead of RNA sample was used to check if there was any contamination.…”

“…It was first isolated from Strobilanthes cusia as an anti-fungal agent against dermatophytes (7). Tryptanthrin is a weakly basic alkaloid and has been reported to have various biological and pharmacological activities, such as inhibitory activities against a variety of microorganisms and parasites (7)(8)(9)(10), immunomodulatory (11)(12)(13) and anti-inflammatory activities (13)(14)(15), and anti-tumor activity towards human leukemia and breast cancer cell lines in vitro (16)(17)(18). Moreover, the crude ethyl acetate extract and tryptanthrin extracted from the indigo plant Polygonum tinctorium have cancer chemopreventive activity by prevention of azoxymethane-induced intestinal tumor formation in F344 rats (19).…”