The nature of immunoglobulin idiotypes and idiotype‐anti‐idiotype interactions in immunological networks

Poskitt, D.C.; Jean-Francois, M. J. B.; Turnbull, S.; Macdonald, L.; Yasmeen, D.

doi:10.1038/icb.1991.11

Cited by 12 publications

(6 citation statements)

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“…Anti‐Ids that recognize a common Id and represent an internal image of the antigen have been generated in many systems [25,28]. Their antigenic mimicry makes then valuable as substitutes not only for certain hormones, e.g.…”

Section: Discussionmentioning

confidence: 99%

“…Although it is generally assumed that anti-Id antibodies which arise in an intact network will reduce immune responses associated with that particular idiotype, anti-idiotypic networks may also have the capacity to increase immune response [26,27]. Anti-Ids that recognize a common Id and represent an internal image of the antigen have been generated in many systems [25,28]. Their antigenic mimicry makes then valuable as substitutes not only for certain hormones, e.g.…”

Section: Reaction Of Ab2 With Heterologous Seramentioning

confidence: 99%

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B and T cell responses elicited by monoclonal anti-idiotypic antibody (Ab2β) mimicking gp43 fromParacoccidioides brasiliensis

Souza

Lopes

Almeida

2004

Clinical and Experimental Immunology

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SUMMARYParacoccidioidomycosis is a systemic mycosis endemic in Latin America, with a high prevalence in Brazil, Argentina, Colombia and Venezuela. The aetiological agent of disease is the thermal dimorphic fungus, Paracoccidioides brasiliensis . A glycoprotein of 43 kD (gp43) is the major antigen of P. brasiliensis . Antibodies directed to this antigen are detected in the sera of all patients with paracoccidioidomycosis (PCM). Recently, it has been shown that mice immunized with anti-gp43 monoclonal antibodies (MAbs) (Ab1), induce the idiotypic cascade in the gp43 system, which produced both, anti-Id antibodies (Ab2) and anti-anti-Id antibodies (Ab3). To further characterize the idiotypic cascade modulation in mice immunized with anti-gp43 MAb 17c, hybridomas were produced. Ab2 MAbs named 7.B12 inhibited ( > 95%) the binding of gp43 to MAb 17c (Ab1), suggesting that this anti-Id MAb bind to the idiotope, thus fulfilling the internal image criteria. To elucidate whether Ab2 MAb could act as antigen in serological assays, instead of gp43, sera from PCM patients were tested. Using an ELISA test, it was observed that antibodies from patients and not normal serum bound to Ab2. However, the ELISA test using Ab2 bound to the solid phase made possible to serologically monitor the patients after antifungal therapy, showing an equivalent curve when compared with ELISA test employing purified gp43. Our results also showed that, when mice were immunized with Ab2 b and their cells were exposed to gp43 in vitro , a T cell proliferation response was observed.

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Section: Discussionmentioning

confidence: 99%

Section: Reaction Of Ab2 With Heterologous Seramentioning

confidence: 99%

B and T cell responses elicited by monoclonal anti-idiotypic antibody (Ab2β) mimicking gp43 fromParacoccidioides brasiliensis

Souza

Lopes

Almeida

2004

Clinical and Experimental Immunology

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“…The idiotype is composed of a set of antigenic determinants or idiotopes (Poskitt et al, 1991b). The idiotope can be located on the variable light chain (Pasquali et al, 1987), the heavy chain (Parhami-Seren et al, 1990) or can result from the interaction of the two chains (Bentley et al, 1990).…”

Section: The Concept Of Idiotypymentioning

confidence: 99%

Anti-idiotypic antibodies as cancer vaccines: achievements and future improvements

Ladjemi¹

2012

Front. Oncol.

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Since the discovery of tumor-associated antigens (TAAs), researchers have tried to develop immune-based anti-cancer therapies. Thanks to their specificity, monoclonal antibodies (mAbs) offer the major advantage to induce fewer side effects than those caused by non-specific conventional treatments (e.g., chemotherapy, radiotherapy). Passive immunotherapy by means of mAbs or cytokines has proved efficacy in oncology and validated the use of immune-based agents as part of anti-cancer treatment options. The next step was to try to induce an active immune protection aiming to boost own’s host immune defense against TAAs. Cancer vaccines are thus developed to specifically induce active immune protection targeting only tumor cells while preserving normal tissues from a non-specific toxicity. But, as most of TAAs are self antigens, an immune tolerance against them exists representing a barrier to effective vaccination against these oncoproteins. One promising approach to break this immune tolerance consists in the use of anti-idiotypic (anti-Id) mAbs, so called Ab2, as antigen surrogates. This vaccination strategy allows also immunization against non-proteic antigens (such as carbohydrates). In some clinical studies, anti-Id cancer vaccines indeed induced efficient humoral and/or cellular immune responses associated with clinical benefit. This review article will focus on recent achievements of anti-Id mAbs use as cancer vaccines in solid tumors.

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Section: Muramyldipeptidementioning

confidence: 99%

“…According to this theory for each exogeneous epitope an anti-id antibody exists mimicking the so-called internal image of this epitope by its hypervariable region. This antibody is designated as Ab2/3 [12].Anti-id antibodies can be induced by immunization with anti-ligand antibodies, imitating the binding site of the receptor. Using this approach monoclonal and polyclonal antibodies to a number of receptors were produced [13-161.…”

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confidence: 99%

Production and characterization of monoclonal anti‐idiotypic antibodies to muramylpeptide

et al. 1994

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Hybridomas producing monoclonal anti-idiotypic antibodies (anti-id MAbs) to N-acetylglucosaminyl-B1-4-N_acetylmur~yl-~~yl-~-isoglutamine (GMDP) were developed. Three clones of hybridomas demonstrated the properties characteristic for the Ab2# type of anti-id antibody: they bound to Fab-fragments of high-affinity MAb to GMDP, dosedependent inhibition of this binding by GMDP was observed; immunization of mice with these MAbs resulted in production of GMDP-specific antibodies. When these antibodies were used to stain blots from SDS-PAGE of macrophage lysate, the same receptor proteins were specifically stained as upon staining with 'zsI-labelled GMDP derivative. Muramyldipeptide(MDP) is the minimal structure of the bacterial cell wall capable of replacing mycobacteria in Freunds' complete adjuvant [l]. MDP and its analogues, containing the N-acetylglucosamine residue, demonstrate a variety of biological activities, in particular, adjuvant effect [2,3], induction of non-specific resistance to infections [4,5], and som-.nogenic activity [6]. The molecular basis of biological activity of mummy1 peptides (MPs) is poorly understood, but the majority of data favors a receptor-mediated mechanism [7-lo].Macrophages are shown to be the main target of MPs [4]. The number of cell surface receptors for MPs is low, making their isolation and structural analysis difficult [7,8]. Like in studies of receptors of other biologically active ligands, monoclonal antibodies to GMDP-receptors could be of great aid, but their production by traditional procedures is hardly achieved.Not long ago an alternative approach was developed based on the production of anti-idiotypic antibodies (anti-id) to corresponding ligands, capable of binding to ligand receptors. This approach is based on the Jeme theory of idiotypic network, claiming that the immune system consists of a set of interacting idiotypes and anti-idiotypes [ 111. According to this theory for each exogeneous epitope an anti-id antibody exists mimicking the so-called internal image of this epitope by its hypervariable region. This antibody is designated as Ab2/3 [12].Anti-id antibodies can be induced by immunization with anti-ligand antibodies, imitating the binding site of the receptor. Using this approach monoclonal and polyclonal antibodies to a number of receptors were produced [13-161. The crucial step in obtaining anti-id MAbs capable of binding to the ligand *Corresponding author. Fax: (7) (095) 310 7007.Abbreviations: MP, mummy1 peptide; MDP, MurNAc-Ala-n-iGln; GMDP, GlcNAc-/314MurNAc-Ala-D-iGln; L-GMDP, GlcNAc#?1-4-MurNAc-Ala-iGin; GMDP-Lys, GlcNAc-/714-MurNAoAla-n-iGlnLys; PBS, 0.01 M sodium phosphate buffer, containing 0.15 M NaCl; PBST, PBS containing 0.1% Tween 20; BSA, bovine serum albumin; GMDP-OVA, GMDP conjugated to ovalbumin; GMDP-AL, GMDP conjugated to poly-Lys-poly-Ala polymer; MeBSA, methylated bovine serum albumin.receptor is identifying among hybridoma those clones producing antibodies of the Ab2# type to paratope-associated epitopes. In this study we ...

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The nature of immunoglobulin idiotypes and idiotype‐anti‐idiotype interactions in immunological networks

Cited by 12 publications

References 83 publications

B and T cell responses elicited by monoclonal anti-idiotypic antibody (Ab2β) mimicking gp43 fromParacoccidioides brasiliensis

B and T cell responses elicited by monoclonal anti-idiotypic antibody (Ab2β) mimicking gp43 fromParacoccidioides brasiliensis

Anti-idiotypic antibodies as cancer vaccines: achievements and future improvements

Production and characterization of monoclonal anti‐idiotypic antibodies to muramylpeptide

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