The nature of the – transition of DNA in solution

Маленков, Г. Г.; Minchenkova, L. E.; Minyat, E. E.; Schyolkina, A. K.; Иванов, В. И.

doi:10.1016/0014-5793(75)80850-7

Cited by 71 publications

(47 citation statements)

References 16 publications

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“…The prominent CD maximum recorded for 20.0 mM nickel concentration at temperatures intermediate between room and melting, suggests the formation of either an A-form of polyAT or C(1) aggregates, [61][62][63][64][65][66][67] both exhibiting a strong CD maximum, at 260 nm for the canonical A-form of polyAT 65 and at about 280 nm for the C(1) form obtained in the ethanol (33.6%)-NaClO 4 (0.61 M) solvent at 267 K. 63 However it is well known that the A-form of polyAT can be obtained only at low temperatures 61 and that the B-to-A transition is inhibited by even very low concentrations of nickel. 63 A typical feature of the A-form, in addition, is a strong CD minimum in the region 210-220 nm.…”

Section: Discussion and Conclusion Effects Of Nicl 2 On Polyatmentioning

confidence: 95%

Circular dichroism of polynucleotides: Interactions of NiCl₂ with poly(dA‐dT)·poly(dA‐dT) and poly(dG‐dC)·poly(dG‐dC) in a water‐in‐oil microemulsion

et al. 2008

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The thermal behavior of the synthetic, high molecular weight, double stranded polynucleotides poly(dA-dT).poly(dA-dT) [polyAT] and poly(dG-dC).poly(dG-dC) [polyGC] solubilized in the aqueous core of the quaternary water-in-oil cationic microemulsion CTAB|n-pentanol|n-hexane|water in the presence of increasing amounts of NiCl(2) at several constant ionic strength values (NaCl) has been studied by means of circular dichroism and electronic absorption spectroscopies. In the microemulsive medium, both polynucleotides show temperature-induced modifications that markedly vary with both Ni(II) concentration and ionic strength. An increase of temperature causes denaturation of the polyAT duplex at low nickel concentrations, while more complex CD spectral modifications are observed at higher nickel concentrations and ionic strengths. By contrast, thermal denaturation is never observed for polyGC. At low Ni(II) concentrations, the increase of temperature induces conformational transitions from B-DNA to Z-DNA form, or, more precisely, to left-handed helical structures. In some cases, at higher nickel concentrations, the CD spectra suggest the presence of Z'-type forms of the polynucleotide.

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Section: Discussion and Conclusion Effects Of Nicl 2 On Polyatmentioning

confidence: 95%

Circular dichroism of polynucleotides: Interactions of NiCl₂ with poly(dA‐dT)·poly(dA‐dT) and poly(dG‐dC)·poly(dG‐dC) in a water‐in‐oil microemulsion

et al. 2008

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“…Previous studies identified no exciplex formation in aqueous conditions because the greater stacking of the bases and increasing the hydrophobic interactions would stabilise the B-form in a medium with high water activity. In a medium with low water activity this hydrophobic effect is less important and the A-form is favoured [39]. It is therefore possible that in the 80% TFE we used to study the exciplex the tandem duplex exists in the A-form.…”

Section: Discussionmentioning

confidence: 99%

New Concepts of Fluorescent Probes for Specific Detection of DNA Sequences: Bis-Modified Oligonucleotides in Excimer and Exciplex Detection

Gbaj¹,

Bichenkova²,

Walsh³

et al. 2009

Libyan J Med

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The detection of single base mismatches in DNA is important for diagnostics, treatment of genetic diseases, and identification of single nucleotide polymorphisms. Highly sensitive, specific assays are needed to investigate genetic samples from patients. The use of a simple fluorescent nucleoside analogue in detection of DNA sequence and point mutations by hybridisation in solution is described in this study. The 5′-bispyrene and 3′-naphthalene oligonucleotide probes form an exciplex on hybridisation to target in water and the 5′-bispyrene oligonucleotide alone is an adequate probe to determine concentration of target present. It was also indicated that this system has a potential to identify mismatches and insertions. The aim of this work was to investigate experimental structures and conditions that permit strong exciplex emission for nucleic acid detectors, and show how such exciplexes can register the presence of mismatches as required in SNP analysis. This study revealed that the hybridisation of 5′-bispyrenyl fluorophore to a DNA target results in formation of a fluorescent probe with high signal intensity change and specificity for detecting a complementary target in a homogeneous system. Detection of SNP mutations using this split-probe system is a highly specific, simple, and accessible method to meet the rigorous requirements of pharmacogenomic studies. Thus, it is possible for the system to act as SNP detectors and it shows promise for future applications in genetic testing.

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“…It was shown in previous work, that AHH2o in the B to A transition concentration range in the temperature interval 0-70°C changes linearily with temperature [8,9]. Thus we can write, for the dependence of activity coefficients on temperature, (12) In Influence of temperature on the B to A transition in water-ethanol solutions has been studied by two groups of authors [ 2 , 2 6 ] .…”

Section: Hydrogen Bonding and Epr Polarity Criterionmentioning

confidence: 95%

“…As was shown in the early x-ray s s @ 1979 John Wiley & Sons, Inc. 0020-7608/79/00 16-0655 $0 1 .OO studies of DNA, the B to A transition is induced by decrease of relative humidity in the atmosphere surrounding DNA fibers [6]. It is very convenient to follow this transition in the solution by CD spectroscopy [2,8]. A detailed study of the B to A transition in the solutions has been performed by Ivanov and co-workers, the main results of which have been summarized in the paper presented here [l].…”

Section: N -S N -Smentioning

confidence: 96%

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Influence of intermolecular interactions on the DNA conformation

Маленков¹,

Gagua²,

Timofeev³

1979

Int J of Quantum Chemistry

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Of the two known families of double-stranded DNA conformations, A is stable in less-polar, and B in more-polar solutions. In some water-nonelectrolyte solutions, B to transition occurs when water activity in the system is near 0.8. In such systems, however, as water-methanol, water-ethylene glycol, etc., B conformation is stable at very low water activity. Hyperfine splittingconstant of a spin label ( A ) , a widely used criterion of the solvent polarity, was measured by EPR method in the solutions, in which fi to A transition takes place at 25°C. A values in solutions of different compositions are close to each other. A method for quantitative estimation of Van der Waals and hydrogen-bond contributions to A values is proposed. A values in water-methanol solutions show that their polarity is too high for the A form to be stable. Decreasing temperature shifts the B-A equilibrium to the B form, which correlates with increasing polarity of the water-alcohol solutions, as measured by the EPR method. Thus B to A transition is mainly determined by the polarity of the environment, which, in turn, is determined by the ability of the solvent molecules to participate in Van der Waals interactions and hydrogen bonding.

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The nature of the – transition of DNA in solution

Cited by 71 publications

References 16 publications

Circular dichroism of polynucleotides: Interactions of NiCl₂ with poly(dA‐dT)·poly(dA‐dT) and poly(dG‐dC)·poly(dG‐dC) in a water‐in‐oil microemulsion

Circular dichroism of polynucleotides: Interactions of NiCl₂ with poly(dA‐dT)·poly(dA‐dT) and poly(dG‐dC)·poly(dG‐dC) in a water‐in‐oil microemulsion

New Concepts of Fluorescent Probes for Specific Detection of DNA Sequences: Bis-Modified Oligonucleotides in Excimer and Exciplex Detection

Influence of intermolecular interactions on the DNA conformation

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The nature of the – transition of DNA in solution

Cited by 71 publications

References 16 publications

Circular dichroism of polynucleotides: Interactions of NiCl2 with poly(dA‐dT)·poly(dA‐dT) and poly(dG‐dC)·poly(dG‐dC) in a water‐in‐oil microemulsion

Circular dichroism of polynucleotides: Interactions of NiCl2 with poly(dA‐dT)·poly(dA‐dT) and poly(dG‐dC)·poly(dG‐dC) in a water‐in‐oil microemulsion

New Concepts of Fluorescent Probes for Specific Detection of DNA Sequences: Bis-Modified Oligonucleotides in Excimer and Exciplex Detection

Influence of intermolecular interactions on the DNA conformation

Contact Info

Product

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Circular dichroism of polynucleotides: Interactions of NiCl₂ with poly(dA‐dT)·poly(dA‐dT) and poly(dG‐dC)·poly(dG‐dC) in a water‐in‐oil microemulsion

Circular dichroism of polynucleotides: Interactions of NiCl₂ with poly(dA‐dT)·poly(dA‐dT) and poly(dG‐dC)·poly(dG‐dC) in a water‐in‐oil microemulsion