The Nephroprotective Effect of Lycopene on Renal Ischemic Reperfusion Injury: A Mouse Model

Abstract

Acute kidney injury (AKI) is characterized by fast decline in renal function within a short period of time. Renal ischemic-reperfusion (I-R) injury is the main cause of AKI. This study aims to investigate the possible nephroprotective effect of lycopene on renal ischemicreperfusion injury in mice model. Forty Swiss Albino adult male mice were randomly allocated onto one of the four study groups: sham group: mice had median laparotomy under anesthesia with no procedures performed, renal tissues and blood samples were collected. ischemic-reperfusion group (I-R-control): mice underwent median laparotomy under anesthesia, followed by 30 min bilateral renal ischemia. Renal tissues and blood samples were collected after 2 h from reperfusion. Vehicle-treated group: mice were pretreated with intra 1% dimethyl sulfoxide 30 min before inducing ischemia. Lycopene-treated group: mice were pretreated with 10 mg/kg intraperitoneal injection of lycopene 30 min before inducing renal ischemia. Renal tissues, and blood samples were collected after 2 h from reperfusion. Blood and tissue samples were collected to look for evidence of inflammation and necrosis. Blood urea nitrogen, serum creatinine as well as plasma NGAL levels were significantly increased in the active control group (P B 0.05), when compared to the sham group. Similarly, renal levels of Notch2/Hes 1, TLR 2, IL-6, Bax, and F2-isoprostane were significantly increased in the active control group as compared to the sham group (P B 0.05). Moreover, lycopene treatment was found to be significantly effective in reducing the increased levels of these markers after I-R injury (P B 0.05).

Potential nephroprotective effects of angiotensin II type 2 receptor agonist Compound 21 in renal ischemia-reperfusion injury

“… Sham group: Underwent anesthesia followed by a midline laparotomy without exposure to I/R. Control group: Subjected to 30 minutes of renal ischemia via artery clamping, followed by 120 minutes of reperfusion [ 9 ]. Vehicle group: Received an intraperitoneal injection of Dimethyl sulfoxide (DMSO), the solvent for Compound 21, 30 minutes before I/R [ 10 ].…”

Effects of Lycopene Attenuating Injuries in Ischemia and Reperfusion

“…A recent study found that before renal I/R injury (30 min/2 h), intake of lycopene (at 30 min before ischemia, 10 mg/kg, i.p.) reduced the levels of urea and creatinine in the serum and improved pathologic changes to renal tissues and cells by inhibiting the Notch2/ Hes-1 pathway; decreasing Bax, IL-6, and TLR-2; and increasing Bcl-2 [145]. Another study showed that in the renal I/R injury (45 min/6 h), lycopene pretreatment (at 30 min before ischemia, 100 mg/kg, i.g.)…”

Plants with Therapeutic Potential for Ischemic Acute Kidney Injury: A Systematic Review

“…The biomarkers NGAL and KIM-1 are considered to diagnose AKI in its early stages compared to other surrogate biomarkers [ 201 , 202 ]. Among the plant-derived extracts and compounds which lowered NGAL and KIM-1 levels are quercetin [ 155 ], lycopene [ 140 ] and α -bisabolol [ 29 ], oleanolic acid [ 85 ], as well as acai fruit extracts [ 112 ], respectively ( Table 1 ).…”