The neuroprotective and lifespan-extension activities of Damnacanthus officinarum extracts in Caenorhabditis elegans

Yang, Xiliang; Zhang, Peng; Wu, Jizhou; Xiong, Siqin; Jin, Nanxiang; Huang, Zebo

doi:10.1016/j.jep.2012.01.025

Cited by 35 publications

(26 citation statements)

References 15 publications

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“…188.23, 95% purity) was purchased from Lancaster Synthesis Ltd. (Newgate Morecambe, UK), dissolved in dimethyl sulfoxide (DMSO) to 1 M, and stored at −20°C as a master stock solution. A food clearance test was used to determine the impact of n -butylidenephthalide on C. elegans physiology [55], [56]. A culture of E. coli was grown overnight and then resuspended at a final optical density (OD) of 6.6 in nematode S-medium [56].…”

Section: Methodsmentioning

confidence: 99%

n-Butylidenephthalide Protects against Dopaminergic Neuron Degeneration and α-Synuclein Accumulation in Caenorhabditis elegans Models of Parkinson's Disease

Harn

Liu

et al. 2014

PLoS ONE

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BackgroundParkinson's disease (PD) is the second most common degenerative disorder of the central nervous system that impairs motor skills and cognitive function. To date, the disease has no effective therapies. The identification of new drugs that provide benefit in arresting the decline seen in PD patients is the focus of much recent study. However, the lengthy time frame for the progression of neurodegeneration in PD increases both the time and cost of examining potential therapeutic compounds in mammalian models. An alternative is to first evaluate the efficacy of compounds in Caenorhabditis elegans models, which reduces examination time from months to days. n-Butylidenephthalide is the naturally-occurring component derived from the chloroform extract of Angelica sinensis. It has been shown to have anti-tumor and anti-inflammatory properties, but no reports have yet described the effects of n-butylidenephthalide on PD. The aim of this study was to assess the potential for n-butylidenephthalide to improve PD in C. elegans models.Methodology/Principal FindingsIn the current study, we employed a pharmacological strain that expresses green fluorescent protein specifically in dopaminergic neurons (BZ555) and a transgenic strain that expresses human α-synuclein in muscle cells (OW13) to investigate the antiparkinsonian activities of n-butylidenephthalide. Our results demonstrate that in PD animal models, n-butylidenephthalide significantly attenuates dopaminergic neuron degeneration induced by 6-hydroxydopamine; reduces α-synuclein accumulation; recovers lipid content, food-sensing behavior, and dopamine levels; and prolongs life-span of 6-hydroxydopamine treatment, thus revealing its potential as a possible antiparkinsonian drug. n-Butylidenephthalide may exert its effects by blocking egl-1 expression to inhibit apoptosis pathways and by raising rpn-6 expression to enhance the activity of proteasomes.Conclusions/Significance n-Butylidenephthalide may be one of the effective neuroprotective agents for PD.

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Section: Methodsmentioning

confidence: 99%

n-Butylidenephthalide Protects against Dopaminergic Neuron Degeneration and α-Synuclein Accumulation in Caenorhabditis elegans Models of Parkinson's Disease

Harn

Liu

et al. 2014

PLoS ONE

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“…5 Aqueous as well as organic fractions of the extracts from Damnacanthus officinarum roots and leaves are also shown to extend the life span of an HA759 strain nematode, a polyQ150-expressing model. 6 Oxidative Stress Resistance As a Prognostic Indicator of Anti-Aging Capability Enhanced resistance to oxidative stress has been shown to extend the C. elegans life span in several studies and thus can be used to assess the anti-aging potential of herbal extracts. Recently, we have used C. elegans to evaluate polysaccharides against paraquat-and hydrogen peroxide (H 2 O 2 )-induced oxidative stress.…”

Section: Life Span Extension As An Unequivocal Anti-aging Indexmentioning

confidence: 99%

“…5 Using this assay, we found that astragalan and some fractions of D. officinarum extracts are effective in protection of the nematodes from polyQ-mediated ASH neurodegeneration. 5,6 Rescue of Behavioral Deficits As a Necessity to Achieve Relief from Neurodegenerative Diseases…”

Section: Suppression Of Neuronal Proteotoxicity As An Indication Of Amentioning

confidence: 99%

“…For example, the n-butanol fraction of D. officinarum extracts is found to increase the avoidance capacity of HA759 nematodes to high osmolarity. 6 In addition to transgenic models, chemically induced C. elegans models are also readily available. For example, the neurotoxic compound 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) and its active metabolite 1-methyl-4-phenylpyridine (MPP + ) are known to induce PD symptoms.…”

Section: Suppression Of Neuronal Proteotoxicity As An Indication Of Amentioning

confidence: 99%

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Caenorhabditis elegans in Chinese Medicinal Studies: Making the Case for Aging and Neurodegeneration

Wang

Yang

Guo

et al. 2014

Rejuvenation Research

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Aging is a progressive process with degenerative changes of various tissues and organs. As a classic model organism in genetics and neurobiology, Caenorhabditis elegans is also a powerful system in aging and behavioral studies and can be used at both the molecular and organismal levels to evaluate potential therapeutics for age-related neurodegeneration, owing to its short life span, relative simplicity, and high degree of experimental tractability as well as significant conservation of disease genes and signaling pathways with humans. We attempt here to summarize the use of C. elegans models in exploring traditional Chinese medicine for potential remedies against aging and neurodegeneration.

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“…Some of these medicines are shown to extend life span or alleviate age-associated diseases in animal models including mice and Caenorhabditis elegans. 1,2 In practice, Chinese medicines are often extracted with water and used as a mixture, with decoction as the most common dosage form. As a result, edible anti-aging Chinese medicines are also traditionally used in functional beverages on the basis of their long and safe usage.…”

Section: Introductionmentioning

confidence: 99%

Overview of Beverages with Anti-Aging Functions in Chinese Market

Yang

Liu

Ming

et al. 2014

Rejuvenation Research

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Anti-aging Chinese medicines have been used in traditional beverages to promote health and prevent diseases. Interestingly, these functional beverages may be used differently between men and women, reflecting the ''yin-yang'' philosophy of Chinese medicine. Modern studies have revealed that some dietary natural products can slow aging in model organisms, and functional beverages containing such products have recently emerged in Chinese market, challenging the dominance of traditional functional beverages. Here we summarize both traditional anti-aging herbs and modern natural dietary compounds currently used in functional beverages in China, and also briefly outline the underlying mechanisms of the beverages in slowing aging process.

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The neuroprotective and lifespan-extension activities of Damnacanthus officinarum extracts in Caenorhabditis elegans

Cited by 35 publications

References 15 publications

n-Butylidenephthalide Protects against Dopaminergic Neuron Degeneration and α-Synuclein Accumulation in Caenorhabditis elegans Models of Parkinson's Disease

n-Butylidenephthalide Protects against Dopaminergic Neuron Degeneration and α-Synuclein Accumulation in Caenorhabditis elegans Models of Parkinson's Disease

Caenorhabditis elegans in Chinese Medicinal Studies: Making the Case for Aging and Neurodegeneration

Overview of Beverages with Anti-Aging Functions in Chinese Market

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