The Neutralizing Characteristics of Strains of Infectious Bronchitis Virus as Measured by the Constant-Virus Variable-Serum Method in Chicken Tracheal Cultures

Johnson, Roy B.; Marquardt, W. W.

doi:10.2307/1588958

Cited by 59 publications

(30 citation statements)

References 17 publications

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“…Traditionally, strain classification into serotypes has been by VNTs performed in various systems, including embryonated eggs (Dawson & Gough, 1971;Cunningham, 1973;Cowen & Hitchner, 1975;Lohr, 1976Lohr, , 1977Gelb et al, 1981Gelb et al, , 1989Gelb et al, , 1991Davelaar et al, 1984;Picault et al, 1986;Karaca et al, 1990;Cubillos et al, 1991;Capua et al, 1998), cell culture (Hopkins, 1974;Cowen & Hitchner, 1975;Wadey & Faragher, 1981;Csermelyi et al, 1988), or TOCs (Johnson & Marquardt, 1975;Darbyshire et al, 1979;Cook, 1984;Cook et al, 1987Cook et al, , 1999Hinze et al, 1991;Ignjatovic & McWaters, 1991;Cubillos et al, 1991;Parsons et al, 1992;Dhinakar Raj & Jones, 1996b;Ignjatovic & Ashton, 1996;Wang et al, 1997). There are several reports on serotyping by the HI test (Lashgari & Newman, 1984;Brown & Bracewell, 1985;Toro et al, 1987;Cook et al, 1987;Capua et al, 1998Capua et al, , 1999.…”

Section: Antigenic Typesmentioning

confidence: 99%

Detection of infectious bronchitis virus

Wit¹

2000

Avian Pathology

167

128

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The detection methods for infectious bronchitis virus (IBV) are reviewed. Advantages and disadvantages of available techniques of IBV detection by virus isolation, antigen or genome detection, and serology are discussed. Factors of influence on the level of success in detection of IBV after a disease outbreak are discussed, as are the possibilities and dangers of strain classification by protectotyping, serotyping, epitope-typing and genotyping.

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Section: Antigenic Typesmentioning

confidence: 99%

Detection of infectious bronchitis virus

Wit¹

2000

Avian Pathology

167

128

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“…So far, group 3 viruses have not been found in humans, but phylogenetic analysis of SARS-coronavirus has shown that its genome contains sequences that seem to be of group 3 origin (Stavrinides & Guttman, 2004). IBV was first found in the USA in 1930 and has since been reported from most countries throughout the four continents of America (Johnson & Marquardt, 1975), Europe (Capua et al, 1994;Cavanagh & Davis, 1993;Gough et al, 1992), Asia (Wang et al, 1997) and Australia (Ignjatovic & McWaters, 1991;Lohr, 1976). Except for 793/ B (or 4/91) (Cavanagh & Naqi, 2003), IBV genotypes are rarely reported to spread from one continent to another (Kusters et al, 1987;Zanella et al, 2003).…”

Section: Introductionmentioning

confidence: 99%

Characterization of a new genotype and serotype of infectious bronchitis virus in Western Africa

Ducatez

Martin

Owoade

et al. 2009

Journal of General Virology

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Between 2002 and 2007, more than 1000 chickens from commercial farms, live bird markets and backyard farms in Nigeria and Niger were tested for the presence of the infectious bronchitis virus (IBV) genome. Phylogenetic analysis of full-length sequences of the spike 1 (S1) gene revealed a new genotype of IBV that we refer to as 'IBADAN'. The minimum genetic distance to the closest 'non-IBADAN' strains (UK/7/93 at the nucleotide level; H120 and M41 at the amino acid level) reached 24 and 32 % at the nucleotide and amino acid levels, respectively. The full genome of the IBADAN reference strain (NGA/A116E7/2006) had a genetic distance of 9.7-16.4 % at the nucleotide level with all available fully sequenced strains. As IBV S1 plays a major role in antigenicity, the antigenic relatedness of NGA/A116E7/2006 was compared with strains of other serotypes. NGA/A116E7/2006 did not cross-react with antisera against IT02, M41, D274, Connecticut or 793/B strains in virus neutralization assays. NGA/A116E7/2006 cross-reacted with the QX-like strain ITA/90254/2005 but only to a low level (antigenic relatedness of 33 %), suggesting that IBADAN also represents a new serotype. A comparison of S1 sequences identified several amino acids that may play a role in IBV antigenicity. Despite the absence of obvious clinical signs in poultry infected by IBADAN strains, it is important to test the crossprotection of current vaccine strains.

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“…Plaque reduction tests in chick kidney cell culture (Hopkins, 1974) and cross-neutralization tests in tracheal organ cultures (Johnson & Marquardt, 1975;Darbyshire et al, 1979;Cook, 1984) have demonstrated the serological diversity among IBV strains. The present studies, however, confirm reports (Hitchner et al, 1964;Raggi & Lee, 1965; that the results of serological tests do not correlate with those of immunological studies and that protection against IBV infection in the chicken is more widely based than the results of in vitro serological tests indicate.…”

Section: Discussionmentioning

confidence: 99%

Infectious Bronchitis Immunity: Its Study in Chickens Experimentally Infected with Mixtures of Infectious Bronchitis Virus and Escherichia coli

Cook¹,

Smith²,

Huggins³

1986

Journal of General Virology

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SUMMARYThe live infectious bronchitis (IB) vaccine, H120, protected chickens against intranasal challenge with a mixture of Escherichia coli strains (E. coli Pool) and IB virus (IBV) strains of the same (Massachusetts) serotype as H120; it usually also protected against challenge with the E. coli Pool and IBV strains of other serological types. When these challenge strains were themselves used as vaccines they usually protected against challenge with a mixture of the E. coli Pool and an IBV strain of the Massachusetts serotype (VF69-149) or an IBV strain not of the Massachusetts serotype (HVI-116). Poor protection, when observed, was most common in those experiments involving a minority of the IBV strains that had been incriminated in recent outbreaks of disease in vaccinated flocks of chickens. Much lower concentrations of IBV strain VF69-149 and E. coli O18 were found in the nose, trachea and spleen of H120-vaccinated chickens killed at different times after they were given a mixture of these organisms than were found in these sites in similarly challenged unvaccinated chickens. Some protection against challenge with IBV and the E. coli Pool was also observed in chickens vaccinated with an inactivated IBV strain; it was much less effective than that obtained following vaccination with the corresponding live IBV strain.

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The Neutralizing Characteristics of Strains of Infectious Bronchitis Virus as Measured by the Constant-Virus Variable-Serum Method in Chicken Tracheal Cultures

Cited by 59 publications

References 17 publications

Detection of infectious bronchitis virus

Detection of infectious bronchitis virus

Characterization of a new genotype and serotype of infectious bronchitis virus in Western Africa

Infectious Bronchitis Immunity: Its Study in Chickens Experimentally Infected with Mixtures of Infectious Bronchitis Virus and Escherichia coli

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