THE new aspects iN MoRPHoloGICAL AND anatomical DiAGNosTics of the herb monarda fistulosa l

С, Лапина А.; Куркин, В. А.; А, Куркин В.; Ryzhov, V.M.; М, Рыжов В.; Тарасенко, Л. В.; В, Тарасенко Л.

doi:10.17816/2072-2354.2019.19.1.19-26

Cited by 3 publications

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“…В научной литературе не описаны особенности люминесценции тканей листьев облепихи крушиновидной. Известно, что люминесцентные особенности тканей позволяют выявлять локализацию биологичес-ки активных структур, а также, в некоторых случаях, проводить селективную диагностику ЛРС [7][8][9][10][11][12][13][14][15]. Люминесцентную микроскопию можно отнести к гистохимическим исследованиям, так как химический состав БАВ в клетках и тканях определяет цвет и интенсивность свечения [7][8][9][10][11][12][13][14][15].…”

Section: методы анализа лекарственных средств Analytical Methodsunclassified

Study of Morphological Features of Sea Buckthorn Leaves by Luminescent and Stereomicroscopy Methods

Kovaleva

Тринеева

Гудкова

et al. 2022

Razrabotka i registraciâ lekarstvennyh sredstv

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Introduction. Sea buckthorn – Hippophaё rhamnoides is a perennial shrub, the fruits of which are a source of carotenoids and are harvested on an industrial scale to obtain a number of drugs. Recently, not only fruits, but also other parts of the plant, such as buds, leaves, shoots and bark, are of increasing interest. It is known that sea buckthorn leaves are rich in compounds such as tannins, polyunsaturated fatty acids and vitamins (C and E). The rich composition of biologically active substances with a sufficient content of components allows us to consider leaves as a new type of medicinal plant material for the further development of various preparations based on them. In addition, the leaves are an annually renewable raw material and it is possible to harvest them on an industrial scale, since this valuable medicinal plant is cultivated everywhere in Russia. Data on the microscopic characteristics of the fruits of sea buckthorn are described in detail in the scientific literature, which cannot be said about the leaves. The scientific literature also does not describe the features of the luminescence of the tissues of the leaves of the sea buckthorn.Aim. The aim of this work was to study the possibility of using the luminescent and stereomicroscopic methods of analysis to determine the main morphological features of the leaves of the sea buckthorn.Materials and methods. Dried whole leaves of a plant of the genus Hippophaës collected in the Voronezh region were used as the object of the study. The study of microdiagnostic signs was carried out according to the State Pharmacopoeia of the XIV RF GPA.1.5.3.0003.15 «Technique of microscopic and microchemical examination of medicinal plants and herbal medicinal products». A stereomicroscopic study was carried out using a Biomed 2 microscope. The luminescence of the tissues of the sea buckthorn leaves was investigated using a luminescent microscope of the Miсromed 3 LUM brand.Results and discussion. Stereomicroscopic analysis of sea buckthorn leaves was carried out. High-resolution volumetric images were obtained in a short period of time. It was found that the characteristic microscopic features of the leaves are the presence of a large number of corymbose hairs along the upper and corymbose-stellate along the lower surfaces, including the vein and petiole (from colorless to white, yellow and dark brown). Single simple stellate hairs are rare. When removing the layer of hairs, the spongy structure of the epidermis of the leaf is visible. The carried out luminescent analysis made it possible to reveal the features of the luminescence of the tissues of the leaves of the sea buckthorn. Diagnostic luminescent features include: the most pronounced yellow glow is observed for the cells of the base of the corymbose hair; the plates that make up the corymbose hairs do not have bright fluorescence; stellate hairs do not have a glow; pronounced yellow-green fluorescence is characteristic of the leaf vein.Conclusion. For the first time, stereomicroscopic and luminescent analyzes of sea buckthorn leaves were carried out and the main morphological features of this type of medicinal plant material and their biometric characteristics were established. The carried out luminescent analysis made it possible to reveal the features of the luminescence of the tissues of the leaves of the sea buckthorn.

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Section: методы анализа лекарственных средств Analytical Methodsunclassified

Study of Morphological Features of Sea Buckthorn Leaves by Luminescent and Stereomicroscopy Methods

Kovaleva

Тринеева

Гудкова

et al. 2022

Razrabotka i registraciâ lekarstvennyh sredstv

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Microdiagnostic characteristics of the herb Artemisia austriaca Jacq. (Artemisia austriaca Jacq.)

Romanteeva,

Kurkin

2024

Farmaciya

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Introduction. Artemisia austriaca Jacq. is a perennial herbaceous plant of the Asteraceae family, popular in folk medicine due to its various biological activities, in particular: wound-healing, anticonvulsant, analgesic, antipyretic and others. In addition, this plant is in the same section as the pharmacopoeial species – wormwood bitter. In the dynamically developing pharmaceutical field, one of the aspects of expanding the base of medicinal plants as sources of phytopreparations is the study of wild plants close to officinal species. One of the initial stages on the way to the development of regulatory documentation for medicinal plant raw materials is the identification of their authenticity criteria that allow reliable identification. Objective: Study of anatomical-diagnostic features of Artemisia austriaca Jacq. of the Saratov region. Material and methods. The objects of the study were samples of Artemisia austriaca Jacq. herbs harvested in 2021 in the Saratov region. The study of the structure of A. austriaca herbs was performed according to the instructions of GPA.1.5.3.0003 "Microscopic and microchemical analysis of medicinal plant raw materials and drugs of plant origin" in the 15th edition of the Russian Federation’s State Pharmacopoeia. Results. The anatomical-histological study of stem, petiole, inflorescence of Artemisia austriaca growing on the territory of Saratov region has been carried out. As a result of the work the markers, luminescent characteristics of tissues, which help to authenticate the raw materials of this taxon, were revealed and clearly presented. Conclusion. The results obtained provide the possibility to identify the mentioned raw material, as an individual, and in addition, in a complex multicomponent phytocomposition.

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Application of Luminescent Microscopy in Analysis of Anatomic-diagnostic Signs of Fruits of Sea Buckthorn

Тринеева

Гудкова

Рудая

2020

Razrabotka i registraciâ lekarstvennyh sredstv

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Introduction. Sea buckthorn (Hippophae rhamnoides), the family Eleagnaceae – a promising source of biologically active substances, the traditional raw materials of which are fruits. The existing regulatory documentation (ND) lacks such an indicator of the authenticity and good quality of the fruit as «microscopy». Despite the available data on the anatomy and histology of the fruits of this type of medicinal plant material (MPM), the scientific literature does not describe the luminescence features of the tissues of sea buckthorn fruit. It is known that the luminescent features of tissues make it possible to identify the localization of biologically active structures, and also, in some cases, to conduct selective diagnosis of MPM.Aim. The aim of this work was to study the characteristics of the luminescence of the tissues of dried crushed fruits of sea buckthorn, and also to optimize the conditions for analysis.Materials and methods. The object of the study was the dried, crushed fruits of sea buckthorn of various species. The study of microdiagnostic signs was carried out according to the State Pharmacopoeia of the XIV RF GPA.1.5.3.0003.15 «Technique of microscopic and microchemical studies of medicinal plant materials and herbal medicines». A stereomicroscopic study was carried out on a Biomed-6 microscope. The luminescence of sea buckthorn fruit tissues was examined using a luminescent microscope of the Micromed-3 Lum brand.Results and discussion. The most pronounced yellow glow is observed for the tissue of the pulp and epidermis of the fetus, which is associated with the highest content of fatty oil in these structures. Groups of phenolic compounds contained along with oil give a greenish tint to objects, while condensed tannins give a brownish tint. The plates that make up the corymbose hairs do not exhibit bright luminescence; a weak glow is characteristic of the joints of the hair platelets. The walls of stellate hairs have a faint greenish glow. Fragments of the actual fruit («sac») are brownish in color, without their own luminescence, as well as the seed peel. The embryo has its own greenish luminescence due to the presence of both a fatty oil and a complex of storage substances.Conclusion. The luminescent analysis of sea buckthorn fruits was carried out for the first time. The choice of a method for preparing the studied MPM for microscopic examination is experimentally substantiated. The luminescent analysis revealed the peculiarities of the glow of the tissues of the fruits of sea buckthorn. The main microdiagnostic signs of crushed dried sea buckthorn fruits and their biometric characteristics are specified. The analysis of the obtained data will allow us to further develop the section «Microscopic signs» for inclusion in the PA project on MPM, widely cultivated and used by the domestic pharmaceutical industry for the production of herbal medicines.

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THE new aspects iN MoRPHoloGICAL AND anatomical DiAGNosTics of the herb monarda fistulosa l

Cited by 3 publications

References 0 publications

Study of Morphological Features of Sea Buckthorn Leaves by Luminescent and Stereomicroscopy Methods

Study of Morphological Features of Sea Buckthorn Leaves by Luminescent and Stereomicroscopy Methods

Microdiagnostic characteristics of the herb Artemisia austriaca Jacq. (Artemisia austriaca Jacq.)

Application of Luminescent Microscopy in Analysis of Anatomic-diagnostic Signs of Fruits of Sea Buckthorn

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