The New Bilayer Lipid Membrane System: Self-Assembling Bilayer Lipid Membranes

Tien, H. Ti; Salamon, Zdzislaw; Guo, Dan; Ottova-Leitmannova, A.

doi:10.1177/1045389x9200300310

Cited by 3 publications

(3 citation statements)

References 25 publications

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“…Transport of Ions. The migration of the periodate ion through the hydrogel was modeled using a coupled transient Nernst−Planck equation , and Poisson equation as a one-dimensional process. The variation in concentrations of the reactants was also incorporated into the model by using the chemical rate equations for the oxidation process. ,− The total flux of ions was predicted using the Nernst−Planck equation which includes the fluxes due to the concentration gradient, electrical migration, and convection of the oxidizing reagent: 18,19 Here, Γ k is the flux of the k th ion, φ is the gel porosity, D̄ k is the effective diffusivity of the k th ion inside the hydrogel, c k is the concentration of the k th ionic species inside the hydrogel, μ̄ k is the effective ionic mobility, z k is the valence of the k th ion, ψ is the electric potential, U is the area-averaged fluid velocity relative to the polymer network, and x is the coordinate system.…”

Section: Mathematical Models and Simulationsmentioning

confidence: 99%

Surface-Modified Hydrogels for Chemoselective Bioconjugation

Plunkett¹,

Chatterjee²,

Aluru³

et al. 2003

Macromolecules

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Patterned hydrogels of glycerol monomethacrylate (GMM)-co-acrylic acid (AA) were prepared in microchannels using an in situ photopolymerization method. Oxidation of the hydrogel was accomplished using aqueous sodium periodate (NaIO 4) to produce an aldehyde functionalized surface. The depth of oxidation, characterized by confocal microscopy after conjugation with a fluorescent dye, was controlled by varying the NaIO4 concentration, reaction time, and temperature. Hydrogel oxidation was modeled by combining the Nernst-Planck, Poisson, Arrhenius, and rate equations. The modeling studies suggest that the glycol oxidation reaction and the diffusion of oxidant into the hydrogel are competitive factors in determining the oxidation depth. Once modified, these hydrogels have the ability to covalently bind small molecules and biomolecules to their surface under mild, aqueous conditions. For example, fluorescently labeled bovine serum albumin (FITC-BSA) was site-specifically conjugated to the surface of a photopatterned hydrogel via reductive amination.

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Section: Mathematical Models and Simulationsmentioning

confidence: 99%

Surface-Modified Hydrogels for Chemoselective Bioconjugation

Plunkett¹,

Chatterjee²,

Aluru³

et al. 2003

Macromolecules

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“…A trans-negative potential (indicated by a minus sign) means that a negative potential was applied to the trans side, the compartment opposite the one where the compounds were added. These experiments are considered to be a sensitive tool to study channel-forming substances [17,18].…”

Section: Electrophysiological Experimentsmentioning

confidence: 99%

Interaction of Quercetin, Cyanidin, and Their O-Glucosides with Planar Lipid Models: Implications for Their Biological Effects

et al. 2023

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Flavonoids are specialized metabolites produced by plants, as free aglycones or as glycosylated derivatives, which are particularly endowed with a variety of beneficial health properties. The antioxidant, anti-inflammatory, antimicrobial, anticancer, antifungal, antiviral, anti-Alzheimer’s, anti-obesity, antidiabetic, and antihypertensive effects of flavonoids are now known. These bioactive phytochemicals have been shown to act on different molecular targets in cells including the plasma membrane. Due to their polyhydroxylated structure, lipophilicity, and planar conformation, they can either bind at the bilayer interface or interact with the hydrophobic fatty acid tails of the membrane. The interaction of quercetin, cyanidin, and their O-glucosides with planar lipid membranes (PLMs) similar in composition to those of the intestine was monitored using an electrophysiological approach. The obtained results show that the tested flavonoids interact with PLM and form conductive units. The modality of interaction with the lipids of the bilayer and the alteration of the biophysical parameters of PLMs induced by the tested substances provided information on their location in the membrane, helping to elucidate the mechanism of action which underlies some pharmacological properties of flavonoids. To our knowledge, the interaction of quercetin, cyanidin, and their O-glucosides with PLM surrogates of the intestinal membrane has never been previously monitored.

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“…The lipid bilayer is the barrier that keeps ions and a great variety of molecules at places where they are needed and prevents them from diffusing into areas where they should not be [1,2].…”

Section: Introductionmentioning

confidence: 99%

Electrochemical Measurements on Supported Phospholipid Bilayers: Preparation, Properties and Ion Transport Using Incorporated Ionophores

et al. 2010

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Model phospholipid bilayers (PLBs) were prepared from 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) and 1,2-dipalmitoyl-sn-glycero-3-phosphoethanolamine (DPPE) on a polycarbonate support and characterized by electrochemical impedance spectroscopy (EIS) measurements. The PLBs were formed by self-assembling in the 8.0 mm holes of the Isopore Membrane Filters, ionophores valinomycin and calcimycin (A23187) were incorporated. The effect of the applied voltage was monitored and reversible changes were observed. The ion transport across supported PLBs (SPLB) from DPPC was studied voltammetrically on the example of the cadmium ion and the ionophore calcimycin. Complex behavior was observed for cadmium ion transport with this ionophore in the presence of calcium ions.

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The New Bilayer Lipid Membrane System: Self-Assembling Bilayer Lipid Membranes

Cited by 3 publications

References 25 publications

Surface-Modified Hydrogels for Chemoselective Bioconjugation

Surface-Modified Hydrogels for Chemoselective Bioconjugation

Interaction of Quercetin, Cyanidin, and Their O-Glucosides with Planar Lipid Models: Implications for Their Biological Effects

Electrochemical Measurements on Supported Phospholipid Bilayers: Preparation, Properties and Ion Transport Using Incorporated Ionophores

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