The New Trend in the Treatment of Experimental Cryptosporidiosis and the Resulting Intestinal Dysplasia

Mostafa, Nabil H.; Hamed, Enas F. Abdel; Fawzy, Eman; Zalat, Rabab; Rashed, Hayam E.; Mohamed, Salem Youssef

doi:10.2217/crc-2018-0008

Cited by 11 publications

(10 citation statements)

References 40 publications

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“…However, adenocarcinoma development was not detected in this model, probably due to the early time of euthanasia. Similar observations were reported by others using the same mice model [60]. Moreover, the severity of the dysplastic lesions seemed to be correlated with the intensity of oocyst shedding [61].…”

Section: Natural or Experimental Infection In Animalssupporting

confidence: 89%

Cryptosporidium and Colon Cancer: Cause or Consequence?

et al. 2020

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The number of cancers attributable to infectious agents represents over 20% of the global cancer burden. The apicomplexan intracellular parasite Cryptosporidium is currently considered one of the major causes of mild and severe diarrhea worldwide. However, less attention has been paid to its tumorigenic potential despite the high exposure of humans and animals to this ubiquitous parasite. Herein, we discuss the potential causal link between Cryptosporidium infection and digestive cancer, with particular emphasis on colon cancer, based on increasing clinical, epidemiological and experimental pieces of evidence supporting this association. In addition, we highlight the current knowledge about the potential mechanisms by which this parasite may contribute to cell transformation and parasite-induced cancer.

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Section: Natural or Experimental Infection In Animalssupporting

confidence: 89%

Cryptosporidium and Colon Cancer: Cause or Consequence?

et al. 2020

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“…In the present study, the immunocompromised mice were treated with combination of NTZ & piperazine citrate 30mg/kg for 7 days started a week post infection, with high significant reduction in C. parvum oocysts that was more or less similar to nitazoxanide treated mice. This agreed with Mostafa et al (2018) in Egypt who found that combination of artesunate and nanazoxide showed a synergistic effect by reducing number of C. parvum oocysts shed and improving dysplastic changes induced by cryptosporidiosis infection in the colon of immunosuppressed mice as compared to that induced by either artesunate or nanazoxide alone. Besides, Checkley et al (2018) in USA reported that diagnostic tests for Cryptosporidium infection were the suboptimum necessitating specialized tests must be often insensitive.…”

Section: Discussionsupporting

confidence: 89%

Parasitological Assessment of Different Concentrations of Piperazine Citrate Against Cryptosporidiosis Infection in Both Immunocompetent and Immunocompromised Mice

Rashed

Eraky

Nageeb

et al. 2021

Journal of the Egyptian Society of Parasitology

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Cryptosporidiosis is one of the risky zoonotic protozoan diseases of worldwide distribution. This present explored the efficacy of different concentrations of piperazine citrate with or without nitazoxanide against cryptosporidiosis infection in immuno-compromised and immuno-competent male mice. One hundred and thirty clean bred male Swiss Albino mice weighed about 20 gm were used. Of them 65 were given immunosuppressive drug (Dexamethasone ® ) for 15 day before infection. All mice put in separate labeled cages in the experimental laboratory under controlled condition and allowed suitable food. After confirming experimental infection, both types of mice were treated with Piperazine citrate as 100mg/kg in different doses (20, 30, & 40mg/kg). Besides, other groups of both types of mice were treated with Nitazoxanide ® (NTZ) syrup 100mg/5ml, or Piperazine 30mg/kg and (NTZ). Assessment of drugs was done by stool examination of modified Zeil-Nelseen stained smears for oocysts.The results showed that both Piperazine citrate and Nitazoxanide caused significant reduction in C. parvum oocysts as compared to control infected non-treated. Immuno-compromised mice treated by piperazine citrate (40mg/kg) for 7 days showed a higher significant reduction in number of oocysts as compared to immunocompromised mice treated by of Piperazine citrate (20&30) mg/ kg for the same period. Combination of Piperazine 30 & NTZ for 7days in immuno-compromised mice gave much more reduction in number of oocysts than NTZ alone (P<0.05). Piperazine 30 & NTZ for 7 days in immuno-compromised mice gave much more reduction in oocysts than NTZ alone (P<0.05).

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“…The pathological picture of examined ilium of different groups were in the form of Limited treatment options also pose a major challenge to public health, giving the significant burden of disease. Those finding come in agreement with [24] who stated that The histopathology of infected colonic mucosa showed marked improvement and low-grade of dysplasia in the infected and treated group with the combined therapy of (artesunate and…”

Section: Necrosis Of Cryptssupporting

confidence: 91%

Effect of piperazine citrate on experimentally cryptosporidium parvum infected mice

Rashed

Eraky

OMAR

et al. 2022

Benha Medical Journal

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Background: Nitazoxanide has not been found to be effective in (Acquired Immunodeficiency Syndrome) patients. Piperazinederivative MMV665917 may potentially be used to treat human cryptosporidiosis, however further investigations are required. Purpose: The aim of this work was to evaluate the effect of piperazine citrate on Cryptosporidium parvum infected mice. Methods: Stool samples would be collected from patients attending Abu-Elrish hospital, showing Cryptosporidium parvum oocyst. One hundred and thirty mice, were selected from (Theodor Bilharz Research Institute). Half of them would take (dexamethasone) for 15 day before infection. Both Nitazoxanide (100mg\kg) and piperazine citrate at different doses (20, 30 & 40 mg\kg) were given to the mice. Assessment of drugs effect was done by parasitological and histopathological assessment. Results: the highest reduction in mean oocysts number of Cryptosporidium parvum was achieved by group treated with combination(nitazoxanide + piperazine 30 ) therapy for 1 week in immunocompromised group (95%) and immunocompetent group (89%) and remarkable regression of histopathological alterations was noticed in ileum of immunocompromised mice treated with combination, when compared with ileum of immunocompromised (positive control) group which showed remarkable histopathological changes exhibited as shortening of the intestinal villi, hyperplasia of goblet cells, inflammatory cells infiltration in the lamina propria , submucosa and edema in the submucosa, multiple Cryptosporidium oocyst .Conclusion: Administration of (piperazine 30+ nitazoxanide) for 7days in immunocompromised group gave the highest reduction rate of mean number of oocysts and remarkable, regression of histopathological alterations.

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The New Trend in the Treatment of Experimental Cryptosporidiosis and the Resulting Intestinal Dysplasia

Cited by 11 publications

References 40 publications

Cryptosporidium and Colon Cancer: Cause or Consequence?

Cryptosporidium and Colon Cancer: Cause or Consequence?

Parasitological Assessment of Different Concentrations of Piperazine Citrate Against Cryptosporidiosis Infection in Both Immunocompetent and Immunocompromised Mice

Effect of piperazine citrate on experimentally cryptosporidium parvum infected mice

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