Toxoplasma surface antigen 1(SAG1) is a major surface antigen involved in the diagnosis of toxoplasmosis. Although it only accounts for 3-5% of total T. gondii protein, the majority of the antibodies are reactive against SAG1 during infection. SAG1 has potent immunogenicity and immune-reactivity; therefore, it is widely applied in diagnostic techniques. Will assess the symptomatic execution of a novel nano symptomatic elisa test Previously, serodiagnosis for human toxoplasmosis. Generation for list 1 What's more its purification might have been carried accompanied Eventually Tom's perusing preparation about igigi polyclonal antibodies Also its purification, characterization Furthermore conjugation with gold nanoparticles identification of Toxoplasma (SAG1) in the serum by nano-gold sandwich elisa uncovered 89. 2% affectability Also 94%, specificity. Nano-gold elisa method is a delicate test to identification of mankind's toxoplasmosis.

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Introduction The accurate diagnosis of toxoplasmosis has critical importance in pregnant women. Nanotechnology and molecular biology are making possible opportunities for accurate and rapid diagnosis of many infectious diseases. Aim and Methods The aim of our study was to compare nano-gold ELISA with ELISA and PCR for diagnosis of toxoplasmosis using Toxoplasma surface antigen grade 1 (SAG1) in pregnant women seeking antenatal care in outpatient clinics. Results PCR showed the highest diagnostic values than nano-gold ELISA and ELISA regarding sensitivity (97.3% versus 89.2% and 83.8%); specificity (100% versus 94% and 88%); and diagnostic accuracy (98.9% versus 91.95% and 86.2%), respectively. There is no statistical difference between PCR and nanogold ELISA results. Discussion Nano-gold ELISA had a significant improvement in diagnosis than the traditional ELISA method. Most likely with the assistance of nanoparticles, more antibodies enter the antigen–antibody complex because of the considerable improvement in the surface area of nano-gold particles. Conclusion Although PCR had higher diagnostic values than nano ELISA, nano ELISA is cheaper and easier than PCR. We recommend nano-gold ELISA with SAG1 as a promising technique in the diagnosis of toxoplasmosis and survey studies.

Trichinellosis is a resurgent parasite that affects people globally. Acid suppressing drugs (ASDs) are among the most frequently prescribed and generally safe medications in many distinct processes (changes defense mechanisms as gut microbiota and immunology). The study evaluated the antacids effect of aluminium hydroxide and magnesium hydroxide in treating Trichinella spiralis in experimental infected mice. Mice (128) were divided into four groups GI: negative control (non-infected untreated), GII: positive control (infected untreated), GIII: mice were infected and treated with albendazole and GIV: mice were infected and treated with antacids. Half of them were sacrificed on 7 th day post infection (PI) for intestinal phase and second half were sacrificed on 35 th day PI for muscular phase. Treatment efficiency was assessed by parasitological, biochemical and histopathological examinations.The results showed that antacids treated mice gave highest number of T. spiralis adult count in intestine and larval count in muscle. Antacids increased the infection (30.5%, 32.5%) respectively compared to infected control. Biochemically showed higher level of IL4, IL10, MDA and lower level of GSH than infected control.