No abstract

Toxoplasma surface antigen 1(SAG1) is a major surface antigen involved in the diagnosis of toxoplasmosis. Although it only accounts for 3-5% of total T. gondii protein, the majority of the antibodies are reactive against SAG1 during infection. SAG1 has potent immunogenicity and immune-reactivity; therefore, it is widely applied in diagnostic techniques. Will assess the symptomatic execution of a novel nano symptomatic elisa test Previously, serodiagnosis for human toxoplasmosis. Generation for list 1 What's more its purification might have been carried accompanied Eventually Tom's perusing preparation about igigi polyclonal antibodies Also its purification, characterization Furthermore conjugation with gold nanoparticles identification of Toxoplasma (SAG1) in the serum by nano-gold sandwich elisa uncovered 89. 2% affectability Also 94%, specificity. Nano-gold elisa method is a delicate test to identification of mankind's toxoplasmosis.

Background: Nanotechnology enables researchers to boost accuracy of the existing diagnostic techniques. Immunomagnetic beads (IMB) based-ELISA was utilized to diagnose several parasitic diseases; schistosomiasis mansoni and japonicum, toxoplasmosis and neurocysticercosis. Objectives: The present work aims to develop and evaluate a novel nano-diagnostic assay using gold nanoparticles (AuNPs) in ELISA (IMB based-ELISA) for the diagnosis of urinary schistosomiasis. Subjects and Methods: IMB based-ELISA assay was developed by preparation of polycolonal antibodies (pAbs) against Schistosoma soluble egg antigen (SEA). The developed novel assay was evaluated in urine samples of 290 schoolchildren collected from primary and preparatory schools in four villages in Beni-Suef governorate, Egypt. Urine samples were screened by chemical reagent strips (Combi 10) and examined by urine microscopy (UM). The conventional ELISA technique was used to evaluate the efficacy of IMB based-ELISA using UM as the gold standard method for diagnosis of urinary schistosomiasis. Results: The novel IMB based-ELISA assay succeeded to diagnose 50 out of 290 schoolchildren (17.2%). In comparison with other methods, results showed that 39/290 (13.4%) were positive by UM and 53/290 (18.3%) by conventional ELISA. A sensitivity, specificity and diagnostic accuracy of the evaluated ELISA assay using UM as the gold standard method were 94.87%, 95.22% and 94.48% respectively. It was observed that Combi-10 gave sensitivity and specificity of 35.9% and 94.9% respectively for microhaematuria and proteinuria. Conclusion: IMB-ELISA based on AuNPs provides a more rapid as well as sensitive detection of SEA in urine samples of patients with active schistosomiasis. Its high sensitivity and specificity ensure its application in field studies. Additionally, urinary schistosomiasis proved highly prevalent in schoolchildren living in Beni-Suef villages.

Cystic echinococcosis (CE) is usually asymptomatic, and commonly presents by pressure symptoms according to its location. Diagnosis of CE is currently based on imaging techniques, which may not be available in some areas and antibody detection in serum, with its known several drawbacks. Conversely, antigen detection has proven its efficacy in serodiagnosis of CE. The study evaluated the efficacy of Nanomagnetic beads (NMB)-sandwich ELISA for diagnosis of CE by detection of Echinococcus (E.) granulosus Antigen B (AgB) in serum and urine samples as compared with sandwich ELISA. A total of 42 individuals were classified into 3 groups; CE infection group, other parasitic infections group and control group. E. granulosus AgB was prepared from human hydatid cysts to produce anti-E. granulosus AgB-immunoglobulin (Ig) Gpoly-clonal Abs in rabbit, after purification, to detect AgB level in serum and urine samples by sandwich ELISA and NMB-sandwich ELISA. Using sandwich ELISA, AgB was detected in sera of CE patients with sensitivity and specificity of 88.9% & 91.7%, respectively. In urine samples, sandwich ELISA detected AgB in CE patients and the sensitivity and specificity were 83.3% and 87.5%, respectively. NMB-sandwich ELISA detected AgB in serum samples of CE patients with a sensitivity and specificity of 94.4% and 95.8%, respectively, whereas in urine samples it was detected in CE cases and the sensitivity and specificity were 88.9% and 95.8%, respectively, without significant statistical difference (p>0.05) between serum and urine samples for detection of AgB when examined by either sandwich ELISA or NMB-sandwich ELI-SA. The best accuracy was obtained with NMB-sandwich ELISA detecting AgB in serum samples (95.2%), with a slightly lower accuracy of 92.9% when using urine samples. NMBsandwich ELISA is efficient for detection of E. granulosus AgB in serum samples. Moreover, urine samples might be a good alternative to serum samples for the detection of AgB.

Introduction The accurate diagnosis of toxoplasmosis has critical importance in pregnant women. Nanotechnology and molecular biology are making possible opportunities for accurate and rapid diagnosis of many infectious diseases. Aim and Methods The aim of our study was to compare nano-gold ELISA with ELISA and PCR for diagnosis of toxoplasmosis using Toxoplasma surface antigen grade 1 (SAG1) in pregnant women seeking antenatal care in outpatient clinics. Results PCR showed the highest diagnostic values than nano-gold ELISA and ELISA regarding sensitivity (97.3% versus 89.2% and 83.8%); specificity (100% versus 94% and 88%); and diagnostic accuracy (98.9% versus 91.95% and 86.2%), respectively. There is no statistical difference between PCR and nanogold ELISA results. Discussion Nano-gold ELISA had a significant improvement in diagnosis than the traditional ELISA method. Most likely with the assistance of nanoparticles, more antibodies enter the antigen–antibody complex because of the considerable improvement in the surface area of nano-gold particles. Conclusion Although PCR had higher diagnostic values than nano ELISA, nano ELISA is cheaper and easier than PCR. We recommend nano-gold ELISA with SAG1 as a promising technique in the diagnosis of toxoplasmosis and survey studies.