2022
DOI: 10.3389/fgene.2021.695763
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The NGS Magic Pudding: A Nanopore-Led Long-Read Genome Assembly for the Commercial Australian Freshwater Crayfish, Cherax destructor

Abstract: Cherax destructor, the yabby, is an iconic Australian freshwater crayfish species, which, similar to other major invertebrate groups, is grossly under-represented in genomic databases. The yabby is also the principal commercial freshwater crustacean species in Australia subject to explotation via inland fisheries and aquaculture. To address the genomics knowledge gap for this species and explore cost effective and efficient methods for genome assembly, we generated 106.8 gb of Nanopore reads and performed a lo… Show more

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“…According to RE studies of Decapoda species included in assembly publications, the proportion of REs varies from 8% to 82% [ 48 , 49 , 50 , 51 , 53 , 54 , 55 , 76 , 77 , 78 , 79 , 80 , 81 , 82 , 83 , 84 ]. Tan et al (2020) annotated the repeatome of eight decapod species and estimated repetitive content between 27% and 50%, with the majority of the genomes having more LINEs, except for P. vannamei , which had more DNA transposons [ 54 ].…”
Section: Resultsmentioning
confidence: 99%
“…According to RE studies of Decapoda species included in assembly publications, the proportion of REs varies from 8% to 82% [ 48 , 49 , 50 , 51 , 53 , 54 , 55 , 76 , 77 , 78 , 79 , 80 , 81 , 82 , 83 , 84 ]. Tan et al (2020) annotated the repeatome of eight decapod species and estimated repetitive content between 27% and 50%, with the majority of the genomes having more LINEs, except for P. vannamei , which had more DNA transposons [ 54 ].…”
Section: Resultsmentioning
confidence: 99%