The NodC protein of Azorhizobium caulinodans is an N-acetylglucosaminyltransferase.

Geremia, R.; Mergaert, Peter; Geelen, Danny; Montagu, Marc Van; Holsters, Marcelle

doi:10.1073/pnas.91.7.2669

Cited by 174 publications

(102 citation statements)

References 26 publications

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“…Chitooligomers of rhizobia are synthesized by the product of the nodC gene, which is known to be a Nacetylglucosaminyl transferase. This enzyme employs UDP-N-acetylglucosamine as substrate to lengthen the oligochitin chain [17][18][19][20]. It seems likely that the R. fredii enzyme is able to replace this intermediate with UDP-glucose, the normal substrate for the synthesis of [3-glucans [21].…”

Section: Discussionmentioning

confidence: 99%

Rhizobium fredii synthesizes an array of lipooligosaccharides, including a novel compound with glucose inserted into the backbone of the molecule

et al. 1996

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Flavonoid cues from the plant host cause symbiotic, nitrogen-fixing rhizobia to synthesize lipochitooligosaccharides (LCOs), which act as return signals to initiate the nodulation process. Rhizobium fredii strain USDA257 is known to produce four LCOs, all substituted with vaccenic acid (Cml). We show here that a mutant strain can replace vaccenic acid with a C16:0 side chain, and that strain USDA191 synthesizes additional LCOs that differ from one another in the length and unsaturation of their fatty acyl substituents. USDA191 and 257DH4 both produce a novel LCO with glucose substituted for N-acetyl-nglucosamine in the backbone of the molecule.

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Section: Discussionmentioning

confidence: 99%

Rhizobium fredii synthesizes an array of lipooligosaccharides, including a novel compound with glucose inserted into the backbone of the molecule

et al. 1996

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“…Although GalNAc-capped chitobiose has previously been observed as a very minor antenna in N-glycans from the serine protease of pit viper venom (24), this is the first report of chito-oligomers in a eukaryotic glycoprotein. The closest homology to these structures in nature are the Nod factors, which are signal molecules produced by Azorhizobium, Bradyrhizobium, and Rhizobium species that trigger nodule formation in leguminous plants (25). Recently it has been shown that Nod-like molecules are FIG.…”

Section: Characterization Of Filarial Nematode N-glycans 20958mentioning

confidence: 99%

Structural Studies of N-Glycans of Filarial Parasites

Haslam

Houston

Harnett

et al. 1999

Journal of Biological Chemistry

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N-Type glycans containing phosphorylcholine (PCglycans), unusual structures found in the important human pathogens filarial nematodes, represent a novel target for chemotherapy. Previous work in our laboratories produced compositional information on the PCglycan of ES-62, a secreted protein of the rodent parasite Acanthocheilonema viteae. In particular, we established using fast atom bombardment mass spectrometry (MS) analysis that PC was attached to a glycan with a trimannosyl core, with and without core fucosylation, carrying between one and four additional N-acetylglucosamine residues. In the present study, we demonstrate that this structure is conserved among filarial nematodes, including the parasite of humans, Onchocerca volvulus, for which new drugs are most urgently sought. Furthermore, by employing a variety of procedures, including collision-activated dissociation MS-MS analysis and matrix-assisted laser desorption MS analysis, we reveal that surprisingly, filarial nematodes also contain N-linked glycans, the antennae of which are composed of chito-oligomers. To our knowledge, this is the first report of such structures in a eukaryotic glycoprotein.

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“…Bacterial strains and plasmids are listed in Table 1, which is published as supporting information on the PNAS web site. A. caulinodans strains were grown overnight at 37°C in yeast extract broth medium (20).…”

Section: Methodsmentioning

confidence: 99%

Lipopolysaccharides as a communication signal for progression of legume endosymbiosis

Mathis

Gijsegem

Rycke

et al. 2005

Proc. Natl. Acad. Sci. U.S.A.

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The NodC protein of Azorhizobium caulinodans is an N-acetylglucosaminyltransferase.

Cited by 174 publications

References 26 publications

Rhizobium fredii synthesizes an array of lipooligosaccharides, including a novel compound with glucose inserted into the backbone of the molecule

Rhizobium fredii synthesizes an array of lipooligosaccharides, including a novel compound with glucose inserted into the backbone of the molecule

Structural Studies of N-Glycans of Filarial Parasites

Lipopolysaccharides as a communication signal for progression of legume endosymbiosis

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